中华耳鼻咽喉头颈外科杂志
中華耳鼻嚥喉頭頸外科雜誌
중화이비인후두경외과잡지
CHINESE JOURNAL OF OTORHINOLARYNGOLOGY HEAD AND NECK SURGERY
2014年
12期
995-1000
,共6页
韩荣荣%周涛%张维芬%赵立民
韓榮榮%週濤%張維芬%趙立民
한영영%주도%장유분%조립민
听觉皮质%神经元%突触%膜片钳术%大鼠,Sprague-Dawley
聽覺皮質%神經元%突觸%膜片鉗術%大鼠,Sprague-Dawley
은각피질%신경원%돌촉%막편겸술%대서,Sprague-Dawley
Auditory cortex%Neurons%Synapses%Patch-clamp techniques%Rats,Sprague-Dawley
目的 观察Sprague-Dawley (SD)大鼠出生后发育过程中,初级听皮层神经元所接收到的突触输入的潜伏期和阈值的变化.方法 采用在体细胞外电生理记录的方法,快速定位初级听皮层大致区域,进一步使用在体细胞封接和全细胞电压膜片钳记录的方法,分别在出生后12 ～ 15 d、16 ～18 d、19 ～ 24 d以及成年(＞3个月)SD大鼠上,记录单个神经元水平上的放电反应以及突触水平上的潜伏期和阈值.结果 ①成年大鼠初级听皮层对于特征频率响应的场电位潜伏期(10～20 ms)较幼年大鼠(20～30 ms)短.②处于发育关键期的幼鼠的单个神经元对白噪声脉冲的放电潜伏期出生后12～ 15 d组[(40.15 ±2.67)ms]和出生后16～18 d组[(33.86 ±4.61)ms]明显长于成年组[(22.93±2.94)]ms,t =4.330、1.995,P=0.00及0.04;而出生后19 ～24 d组[(24.80 ±3.63) ms]与成年组相比,差异无统计学意义(P＞0.05).③幼鼠神经元对于白噪声脉冲兴奋性和抑制性突触输入的潜伏期出生后12 ～ 15 d组[(38.94±1.90) ms,(35.26±2.40) ms]和出生后16～18 d组[(32.68 ±2.52)ms,(30.24±2.18)ms]明显长于成年组[(19.46±1.06)ms,(18.91±0.77) ms],差异具有统计学意义(兴奋性=6.255、4.662,P值均＜0.01;抑制性t=8.918、4.820,P值均＜0.01),出生后19 ～24 d组[(23.67±2.46) ms,(21.43±1.80) ms]与成年组相比,差异无统计学意义(P值均＞0.01);兴奋性输入与抑制性输入潜伏期之间的差值在发育过程中逐渐减小,分别为[(3.15±1.02) ms、(2.01±0.73)ms、(1.79±0.85)ms、(0.39±0.48)ms],出生后12～15 d组与成年组相比,差异具有统计学意义(t=1.739,P＜0.01).④在突触反应阈值方面,幼鼠组[(40.0±1.6)dB,(41.3±1.6)dB,(35.0±2.7)dB]明显高于成年鼠[(30.9±0.6)dB],出生后12 ～15 d、16～ 18 d组与成年组相比,差异具有统计学意义(t =5.284、5.867,P值均＜0.叭),而出生后19～24 d组与成年组差异无统计学意义(P＞0.01).结论 由声刺激诱发的大鼠单个神经元放电活动以及突触反应的潜伏期和阈值均随着皮层发育逐渐达到成熟状态.
目的 觀察Sprague-Dawley (SD)大鼠齣生後髮育過程中,初級聽皮層神經元所接收到的突觸輸入的潛伏期和閾值的變化.方法 採用在體細胞外電生理記錄的方法,快速定位初級聽皮層大緻區域,進一步使用在體細胞封接和全細胞電壓膜片鉗記錄的方法,分彆在齣生後12 ～ 15 d、16 ～18 d、19 ～ 24 d以及成年(＞3箇月)SD大鼠上,記錄單箇神經元水平上的放電反應以及突觸水平上的潛伏期和閾值.結果 ①成年大鼠初級聽皮層對于特徵頻率響應的場電位潛伏期(10～20 ms)較幼年大鼠(20～30 ms)短.②處于髮育關鍵期的幼鼠的單箇神經元對白譟聲脈遲的放電潛伏期齣生後12～ 15 d組[(40.15 ±2.67)ms]和齣生後16～18 d組[(33.86 ±4.61)ms]明顯長于成年組[(22.93±2.94)]ms,t =4.330、1.995,P=0.00及0.04;而齣生後19 ～24 d組[(24.80 ±3.63) ms]與成年組相比,差異無統計學意義(P＞0.05).③幼鼠神經元對于白譟聲脈遲興奮性和抑製性突觸輸入的潛伏期齣生後12 ～ 15 d組[(38.94±1.90) ms,(35.26±2.40) ms]和齣生後16～18 d組[(32.68 ±2.52)ms,(30.24±2.18)ms]明顯長于成年組[(19.46±1.06)ms,(18.91±0.77) ms],差異具有統計學意義(興奮性=6.255、4.662,P值均＜0.01;抑製性t=8.918、4.820,P值均＜0.01),齣生後19 ～24 d組[(23.67±2.46) ms,(21.43±1.80) ms]與成年組相比,差異無統計學意義(P值均＞0.01);興奮性輸入與抑製性輸入潛伏期之間的差值在髮育過程中逐漸減小,分彆為[(3.15±1.02) ms、(2.01±0.73)ms、(1.79±0.85)ms、(0.39±0.48)ms],齣生後12～15 d組與成年組相比,差異具有統計學意義(t=1.739,P＜0.01).④在突觸反應閾值方麵,幼鼠組[(40.0±1.6)dB,(41.3±1.6)dB,(35.0±2.7)dB]明顯高于成年鼠[(30.9±0.6)dB],齣生後12 ～15 d、16～ 18 d組與成年組相比,差異具有統計學意義(t =5.284、5.867,P值均＜0.叭),而齣生後19～24 d組與成年組差異無統計學意義(P＞0.01).結論 由聲刺激誘髮的大鼠單箇神經元放電活動以及突觸反應的潛伏期和閾值均隨著皮層髮育逐漸達到成熟狀態.
목적 관찰Sprague-Dawley (SD)대서출생후발육과정중,초급은피층신경원소접수도적돌촉수입적잠복기화역치적변화.방법 채용재체세포외전생리기록적방법,쾌속정위초급은피층대치구역,진일보사용재체세포봉접화전세포전압막편겸기록적방법,분별재출생후12 ～ 15 d、16 ～18 d、19 ～ 24 d이급성년(＞3개월)SD대서상,기록단개신경원수평상적방전반응이급돌촉수평상적잠복기화역치.결과 ①성년대서초급은피층대우특정빈솔향응적장전위잠복기(10～20 ms)교유년대서(20～30 ms)단.②처우발육관건기적유서적단개신경원대백조성맥충적방전잠복기출생후12～ 15 d조[(40.15 ±2.67)ms]화출생후16～18 d조[(33.86 ±4.61)ms]명현장우성년조[(22.93±2.94)]ms,t =4.330、1.995,P=0.00급0.04;이출생후19 ～24 d조[(24.80 ±3.63) ms]여성년조상비,차이무통계학의의(P＞0.05).③유서신경원대우백조성맥충흥강성화억제성돌촉수입적잠복기출생후12 ～ 15 d조[(38.94±1.90) ms,(35.26±2.40) ms]화출생후16～18 d조[(32.68 ±2.52)ms,(30.24±2.18)ms]명현장우성년조[(19.46±1.06)ms,(18.91±0.77) ms],차이구유통계학의의(흥강성=6.255、4.662,P치균＜0.01;억제성t=8.918、4.820,P치균＜0.01),출생후19 ～24 d조[(23.67±2.46) ms,(21.43±1.80) ms]여성년조상비,차이무통계학의의(P치균＞0.01);흥강성수입여억제성수입잠복기지간적차치재발육과정중축점감소,분별위[(3.15±1.02) ms、(2.01±0.73)ms、(1.79±0.85)ms、(0.39±0.48)ms],출생후12～15 d조여성년조상비,차이구유통계학의의(t=1.739,P＜0.01).④재돌촉반응역치방면,유서조[(40.0±1.6)dB,(41.3±1.6)dB,(35.0±2.7)dB]명현고우성년서[(30.9±0.6)dB],출생후12 ～15 d、16～ 18 d조여성년조상비,차이구유통계학의의(t =5.284、5.867,P치균＜0.팔),이출생후19～24 d조여성년조차이무통계학의의(P＞0.01).결론 유성자격유발적대서단개신경원방전활동이급돌촉반응적잠복기화역치균수착피층발육축점체도성숙상태.
Objective To study the change of synaptic onset latency and threshold in primary auditory cortex (A1) during the development of SD rat.Methods Extracellular recording was used to locate A1,followed by transferred to loose-patch and whole-cell patch in vivo to record the spike activity,synaptic onset latency and threshold responses respectively.Rats were divided into 4 groups according to ages,postnatal 12-15 days,16-18 days,19-24 days and adult (＞ 3 months).Results 1.The onset latency of local field potential in A1 of adult rats [(10-20) ms] was shorter than young rats [(20-30) ms].2.During development,the onset latency of spikes of a single neuron in response to white noise pulses decreased.And the latency in young rats P12-15 [(40.15 ± 2.67) ms] and P16-18 [(33.86 ±4.61) ms] were longer than in adults [(22.93 ± 2.94) ms] (ANOVA-test,t =4.330 and 1.995,P =0.00 and 0.04).However,the onset latencies of P19-24 [(24.80 ± 3.63) ms] and adult had no significant difference (P ＞ 0.05).3.Synaptic onset latencies of both excitation and inhibition were significantly longer in P12-15 [(38.94±1.90)ms,(35.26±2.40)ms] and P16-18[(32.68 ±2.52)ms,(30.24±2.18)ms] than in adults [(19.4 ± 1.06) ms,(18.91 ± 0.77) ms] excitation (t =6.255 and 4.662,P ＜ 0.01) inhibition (t =8.918 and 4.820,P ＜ 0.01) showed significant difference.Whereas the onset latencies of P19-24 [(23.67 ± 2.46) ms,(21.43 ± 1.80) ms] and adults displayed no prominent difference (P ＞ 0.01).Meanwhile,the difference between the onset latencies of excitation and inhibition became narrower during development[(3.15 ± 1.02)ms,(2.01 ±0.73)ms,(1.79 ±0.85) ms,(0.39 ±0.48) ms].P12-15 had notably difference in comparison to adults (t =1.739,P ＜ 0.01).4.The thresholds of synaptic response were notably higher in P12-15(40.0 ± 1.6)dB and P16-18 (41.3 ± 11.6)dB when compared with adults (30.9 ± 0.6) dB (t =5.284 and 5.867,P ＜ 0.01).While that of P19-24 (35.0 ± 32.7) dB showed no distinct difference (P ＞ 0.01).Conclusion Single neuron spiking activity,synaptic onset latency and threshold evoked by sound stimulus gradually mature during the development in rat A1.
