中华耳鼻咽喉头颈外科杂志
中華耳鼻嚥喉頭頸外科雜誌
중화이비인후두경외과잡지
CHINESE JOURNAL OF OTORHINOLARYNGOLOGY HEAD AND NECK SURGERY
2014年
12期
1027-1030
,共4页
舒竞铖%尹时华%刘渊%饶秀丽%韦顺莲
舒競鋮%尹時華%劉淵%饒秀麗%韋順蓮
서경성%윤시화%류연%요수려%위순련
前庭神经核%受体,组胺H3%倍他司汀%豚鼠
前庭神經覈%受體,組胺H3%倍他司汀%豚鼠
전정신경핵%수체,조알H3%배타사정%돈서
Vestibular nuclei%Receptors,histamine H3%Betahistine%Guinea pigs
目的 观察倍他司汀(betahistine)对单侧迷路毁损后的豚鼠前庭内侧核(medial vestibular nucleus)组胺H3受体表达的影响.方法 选择听性脑干反应(ABR)阈值小于40 dBSPL的健康黑色赤目纯种豚鼠56只,采用随机数字表法分为3组:假手术组(8只),单侧迷路破坏组(24只),单侧迷路破坏+倍他司汀组(24只).单侧迷路破坏组和单侧迷路破坏+倍他司汀组均损毁左侧迷路,假手术组手术打开听泡但保持迷路完好.单侧迷路破坏+倍他司汀组损毁迷路后给予倍他司汀2.17 mg/kg腹腔注射,每天1次,连续给药至处死当天;另外两组腹腔注射等量生理盐水.破坏单侧迷路的两组在不同观察时间点(术后1、3、7d)各取8只豚鼠通过免疫组织化学染色观察双侧前庭内侧核组胺H3受体的表达变化.采用SPSS 16.0软件对实验数据进行统计分析.结果 单侧迷路破坏组术后1d及3d,受损侧前庭内侧核组胺H3受体表达增加,与假手术组相比,差异具有统计学意义(P＜0.05),术后7d则恢复至正常水平,与假手术组相比差异无统计学意义(P＞0.05);未损侧前庭内侧核组胺H3受体表达强度各观察时间点与假手术组相比,差异无统计学意义(P值均＞0.05).单侧迷路破坏+倍他司汀组受损侧前庭内侧核组胺H3受体术后1d及3d较同期单侧迷路破坏组表达减弱(4.25 ±0.71、3.50 ±0.92 vs 5.75 ±0.71、5.50 ±0.93,P值均＜0.05);术后3d、7d与假手术组相比差异无统计学意义(P值均＞0.05).结论 豚鼠单侧迷路破坏后同侧前庭内侧核中组胺H3受体表达上调,倍他司汀可促进H3受体表达恢复至正常水平.
目的 觀察倍他司汀(betahistine)對單側迷路燬損後的豚鼠前庭內側覈(medial vestibular nucleus)組胺H3受體錶達的影響.方法 選擇聽性腦榦反應(ABR)閾值小于40 dBSPL的健康黑色赤目純種豚鼠56隻,採用隨機數字錶法分為3組:假手術組(8隻),單側迷路破壞組(24隻),單側迷路破壞+倍他司汀組(24隻).單側迷路破壞組和單側迷路破壞+倍他司汀組均損燬左側迷路,假手術組手術打開聽泡但保持迷路完好.單側迷路破壞+倍他司汀組損燬迷路後給予倍他司汀2.17 mg/kg腹腔註射,每天1次,連續給藥至處死噹天;另外兩組腹腔註射等量生理鹽水.破壞單側迷路的兩組在不同觀察時間點(術後1、3、7d)各取8隻豚鼠通過免疫組織化學染色觀察雙側前庭內側覈組胺H3受體的錶達變化.採用SPSS 16.0軟件對實驗數據進行統計分析.結果 單側迷路破壞組術後1d及3d,受損側前庭內側覈組胺H3受體錶達增加,與假手術組相比,差異具有統計學意義(P＜0.05),術後7d則恢複至正常水平,與假手術組相比差異無統計學意義(P＞0.05);未損側前庭內側覈組胺H3受體錶達彊度各觀察時間點與假手術組相比,差異無統計學意義(P值均＞0.05).單側迷路破壞+倍他司汀組受損側前庭內側覈組胺H3受體術後1d及3d較同期單側迷路破壞組錶達減弱(4.25 ±0.71、3.50 ±0.92 vs 5.75 ±0.71、5.50 ±0.93,P值均＜0.05);術後3d、7d與假手術組相比差異無統計學意義(P值均＞0.05).結論 豚鼠單側迷路破壞後同側前庭內側覈中組胺H3受體錶達上調,倍他司汀可促進H3受體錶達恢複至正常水平.
목적 관찰배타사정(betahistine)대단측미로훼손후적돈서전정내측핵(medial vestibular nucleus)조알H3수체표체적영향.방법 선택은성뇌간반응(ABR)역치소우40 dBSPL적건강흑색적목순충돈서56지,채용수궤수자표법분위3조:가수술조(8지),단측미로파배조(24지),단측미로파배+배타사정조(24지).단측미로파배조화단측미로파배+배타사정조균손훼좌측미로,가수술조수술타개은포단보지미로완호.단측미로파배+배타사정조손훼미로후급여배타사정2.17 mg/kg복강주사,매천1차,련속급약지처사당천;령외량조복강주사등량생리염수.파배단측미로적량조재불동관찰시간점(술후1、3、7d)각취8지돈서통과면역조직화학염색관찰쌍측전정내측핵조알H3수체적표체변화.채용SPSS 16.0연건대실험수거진행통계분석.결과 단측미로파배조술후1d급3d,수손측전정내측핵조알H3수체표체증가,여가수술조상비,차이구유통계학의의(P＜0.05),술후7d칙회복지정상수평,여가수술조상비차이무통계학의의(P＞0.05);미손측전정내측핵조알H3수체표체강도각관찰시간점여가수술조상비,차이무통계학의의(P치균＞0.05).단측미로파배+배타사정조수손측전정내측핵조알H3수체술후1d급3d교동기단측미로파배조표체감약(4.25 ±0.71、3.50 ±0.92 vs 5.75 ±0.71、5.50 ±0.93,P치균＜0.05);술후3d、7d여가수술조상비차이무통계학의의(P치균＞0.05).결론 돈서단측미로파배후동측전정내측핵중조알H3수체표체상조,배타사정가촉진H3수체표체회복지정상수평.
Objective To observe the influence of betahistine on the expression of histamine H3 receptor in the medial vestibular nucleus (MVN) following unilateral labyrinthectomy (UL).Methods Fifty-six healthy guinea pigs were randomly divided into three groups:the sham-operated group (group Ⅰ),the UL group [group Ⅱ,and UL + betahistine (BET) group (group Ⅲ)],BET was intraperitoneally injection at 2.17 mg · kg-1 · d-1 for 7 days.The expression of histamine H3 receptor was analyzed by immunohistochemistry at 1 day,3 days and 7 days after UL.Results H3 receptors were presented in the MVN and the expression of histamine H3 receptor were increased significantly in the ipsilateral MVN at 1 and 3 days after UL(P ＜0.05),the change turned into the normal value at 7 days(P ＞0.05).In the UL + BET group,the intensity of histamine H3 receptor was lower than that in the UL at 1 day and 3 days(4.25 ± 0.71,3.50 ± 0.92 vs 5.75 ± 0.71,5.50 ± 0.93,P ＜ 0.05).However,the changes turned into the normal values at 3 and 7 days (P ＞ 0.05).Conclusions The early stage of the vestibular compensation process may be associated with the change of H3 receptor expression in MVN.In the UL + BET group the histamine H3 receptor recovered quickly.
