CAJ | 학술논문

目的研究氢在晶体关节炎(痛风)中的治疗和(或)保护作用,为痛风的辅助治疗提供新的途径.方法 25只Wistar大鼠,雄性,体质量(200±20)g.采用随机数字表法分为5组,第1组是氢水模型组;第2组是氢饲料模型组;第3组是氢水+氢饲料模型组;第4组为正常对照模型组;第5组是空白组.其中第1组、第2组、第3组称为氢干预模型组.连续喂养14 d后利用尿酸盐晶体混悬液(MSU)注射大鼠踝关节造成大鼠急性痛风性关节炎.连续观察72 h,进行关节肿胀的观察与测量.72 h实验末采血,检测大鼠血清TNF-α、IL-1β及丙二醛.采用单因素方差分析和Tamhane's T2法进行统计.结果通过72 h的观察与测量,发现氢干预模型组的关节肿胀起始时间、肿胀程度、持续时间均优于正常对照模型组,造模后24 h时正常对照模型组大鼠踝关节容积是氢干预模型组的1.24倍(0.40±0.06,P＜0.05).氢干预模型组大鼠血清中TNF-α、IL-1β及丙二醛含量也低于正常对照模型组.正常对照模型组血清TNF-α含量是氢干预模型组的6.23倍(336±60,P＜0.05),正常对照模型组血清IL-1β含量是氢干预模型组的4.02倍(249±42,P＜0.05),正常对照模型组血清丙二醛含量是氢干预模型组的2.18倍(24±4,P＜0.05).结论口服氢对于痛风性关节炎有确切的治疗作用.
목적 연구경재정체관절염(통풍)중적치료화(혹)보호작용,위통풍적보조치료제공신적도경.방법 25지Wistar대서,웅성,체질량(200±20)g.채용수궤수자표법분위5조,제1조시경수모형조;제2조시경사료모형조;제3조시경수+경사료모형조;제4조위정상대조모형조;제5조시공백조.기중제1조、제2조、제3조칭위경간예모형조.련속위양14 d후이용뇨산염정체혼현액(MSU)주사대서과관절조성대서급성통풍성관절염.련속관찰72 h,진행관절종창적관찰여측량.72 h실험말채혈,검측대서혈청TNF-α、IL-1β급병이철.채용단인소방차분석화Tamhane's T2법진행통계.결과 통과72 h적관찰여측량,발현경간예모형조적관절종창기시시간、종창정도、지속시간균우우정상대조모형조,조모후24 h시정상대조모형조대서과관절용적시경간예모형조적1.24배(0.40±0.06,P＜0.05).경간예모형조대서혈청중TNF-α、IL-1β급병이철함량야저우정상대조모형조.정상대조모형조혈청TNF-α함량시경간예모형조적6.23배(336±60,P＜0.05),정상대조모형조혈청IL-1β함량시경간예모형조적4.02배(249±42,P＜0.05),정상대조모형조혈청병이철함량시경간예모형조적2.18배(24±4,P＜0.05).결론 구복경대우통풍성관절염유학절적치료작용.
Objective To investigate the mechanism of hydrogen therapy for gouty arthritis,and provide new strategy for gout via a diet therapy by building up the crystal arthritis (gout) animal model.Methods Wistar rats (200±20) g were randomly divided into five groups which were consisted of five rats,including:the hydrogen-water model group,the hydrogen-feed model group,the hydrogen-water and hydrogenfeed model group,the control model group,and the blank group.And the first,the second,the third group collectively referred to as the hydrogen group.The rat model of acute gouty arthritis was established via injecting monosodium urate (MSU) in rats' ankles,after 14 days continuous feeding.On the third day after injection,serum samples were collected and analyzed.The swelling feet were removed and kept in the 40％ neutral formaldehyde for histochemicalstudies.During the three days after MSU injection,the volume of whole feet (including the ankle joint) was also recorded.The results were analyzed with one-way ANOVA and tamhane's T2 methods.Results In this study,a moderate elevated level of observed parameters,such as swelling joints numbers and inflammatory factor levels,was observed in the hydrogen feed model group than other groups.To measure the volume of rats' feet and found that the volume of feet of the control model group was 1.24 times (0.40±0.06,P＜0.05) as big as hydrogen group.And the TNF-α,IL-1,malondialdehyde in the serum of the hydrogen group were also less than those of the control model group.The contention of TNF-α of the control model group was 6.23 times (336±60,P＜0.05) as much as the hydrogen group.The contention of IL-1β of the control model group was 4.02 times (249±42,P＜0.05) as much as the hydrogen group.The contention of MDA of the control model group was 2.18 times (24±4,P＜0.05) as much as the hydrogen group.Conclusion The oral hydrogen intake has a definite therapeutic effect on controlling articular inflammation,which is helpful in exploring the dietary therapy for gouty arthritis.