CAJ | 학술논문

目的建立检测人血清抗-延胡索酸水合酶(FH)的间接ELISA法,并评价其在自身免疫性肝炎(AIH)临床诊断中的价值.方法采用FH蛋白建立血清抗-FH的间接ELISA法,并对反应条件进行优化.应用建立的ELISA法检测88例AIH、56例PBC、50例慢性乙型肝炎(CHB)、36例慢性丙型肝炎(CHC)和98例健康志愿者(HC)血清中的抗-FH.统计学方法采用x2检验和Kruskal-WallisH法.结果建立了检测人血清抗-FH的间接ELISA法,并确定了反应条件.抗-FH的血清检测结果,在AIH患者中抗-FH的阳性率为40％,显著高于健康志愿者(3％,x2=38.44,P＜0.01)、PBC组(7％,x2=18.45,P＜0.01)、CHB组(2％,x2=23.59,P＜0.01)和CHC组(6％,x2=14.29,P＜0.01).抗-FH对AIH诊断的灵敏度为40％,特异度为94％.结论采用ELISA法检测AIH血清中抗-FH可能提高AIH的诊断,该抗体可能是诊断AIH的新指标.
목적 건립검측인혈청항-연호색산수합매(FH)적간접ELISA법,병평개기재자신면역성간염(AIH)림상진단중적개치.방법 채용FH단백건립혈청항-FH적간접ELISA법,병대반응조건진행우화.응용건립적ELISA법검측88례AIH、56례PBC、50례만성을형간염(CHB)、36례만성병형간염(CHC)화98례건강지원자(HC)혈청중적항-FH.통계학방법채용x2검험화Kruskal-WallisH법.결과 건립료검측인혈청항-FH적간접ELISA법,병학정료반응조건.항-FH적혈청검측결과,재AIH환자중항-FH적양성솔위40％,현저고우건강지원자(3％,x2=38.44,P＜0.01)、PBC조(7％,x2=18.45,P＜0.01)、CHB조(2％,x2=23.59,P＜0.01)화CHC조(6％,x2=14.29,P＜0.01).항-FH대AIH진단적령민도위40％,특이도위94％.결론 채용ELISA법검측AIH혈청중항-FH가능제고AIH적진단,해항체가능시진단AIH적신지표.
Objective To establish the enzyme-linked immunosorbent assay (ELISA) for detecting human serum fumarate hydratase (FH) antibody and evaluate its role in the diagnosis of autoantigen in autoimmune hepatitis (AIH).Methods The indirect ELISA was established using FH protein,and the reaction conditions were determined.Then,the anti-FH antibody were detected in the serum of 88 AIH patients,56 primary biliary cirrhosis (PBC) patients,50 chronic hepatitis B (HBV) patients,36 chronic hepatitis C (HCV) patients and 98 healthy controls(HC).The results were analyzed with chi-quare and Kruskal-Wallis H methods.Results The ELISA for detecting human anti-FH antibody was established successfully and the optimal reaction conditions were defined.The positive rate of anti-FH antibody in the AIH group (40％) was significantly higher than HC (3％,x2=38.44,P＜0.01),PBC group (7％,x2=18.45,P＜0.01),CHB group (2％,x2=23.59,P＜0.01) and CHC group (6％,x2=14.29,P＜0.01).Anti-FH antibody which was used to diagnose AIH revealed a sensitivity of 40％ and specificity of 94％.Conclusion We have established the ELISA,which is used to detect human anti-FH antibody.It can be detected predominantly in AIH,and this implies that anti-FH antibody may be useful in improving the diagnosis of AIH.