中华风湿病学杂志
中華風濕病學雜誌
중화풍습병학잡지
CHINESE JOURNAL OF RHEUMATOLOGY
2014年
12期
836-839
,共4页
张梦云%周京国%青玉凤%潘舒月%蒲梦君%党万太%杨其彬%李玲琴%周畅
張夢雲%週京國%青玉鳳%潘舒月%蒲夢君%黨萬太%楊其彬%李玲琴%週暢
장몽운%주경국%청옥봉%반서월%포몽군%당만태%양기빈%리령금%주창
痛风%端粒重复序列结合蛋白质1%端粒重复序列结合蛋白质2
痛風%耑粒重複序列結閤蛋白質1%耑粒重複序列結閤蛋白質2
통풍%단립중복서렬결합단백질1%단립중복서렬결합단백질2
Gout%Telomeric repeat binding protein 1%Telomeric repeat binding protein 2
目的 探讨端粒重复序列结合蛋白(TRF)1和TRF2在原发性痛风性关节炎(GA)发病中可能的作用.方法 将研究对象分为急性期GA(AGA)组77例、非急性期(NAGA)组49例及健康对照组79名.实时荧光定量多聚合酶链反应(RT-qPCR)及蛋白质印迹法分别检测TRF1和TRF2在3组PBMCs中转录水平及蛋白水平的表达.采用单因素方差分析,LSD法两两比较和Spearman相关分析行统计学处理.结果 ①TRF1、TRF2转录水平及蛋白水平在AGA组(0.012 6±0.005 3和0.017 6±0.007 8)和NAGA组(0.009 5±0.003 6和0.014 0±0.004 1)均显著高于健康对照组(0.004 2±0.001 2和0.005 8±0.0019,F=10.818,F=8.869,P均＜0.01).②在AGA组中TRF2转录水平均与淋巴细胞(LY)呈正相关(r=0.289,P＜0.05),TRF1转录水平与TRF2(r=0.935,P＜0.01)、LY(r=0.256,P＜0.05)、TG (r=0.281,P＜0.05)、极低密度脂蛋白(VLDL-C)(r=0.291,P＜0.05)均呈正相关.结论 端TRF1、TRF2在原发性GA患者的异常表达及与LY呈正相关,提示其可能参与了痛风的发生发展及免疫与炎症调节.
目的 探討耑粒重複序列結閤蛋白(TRF)1和TRF2在原髮性痛風性關節炎(GA)髮病中可能的作用.方法 將研究對象分為急性期GA(AGA)組77例、非急性期(NAGA)組49例及健康對照組79名.實時熒光定量多聚閤酶鏈反應(RT-qPCR)及蛋白質印跡法分彆檢測TRF1和TRF2在3組PBMCs中轉錄水平及蛋白水平的錶達.採用單因素方差分析,LSD法兩兩比較和Spearman相關分析行統計學處理.結果 ①TRF1、TRF2轉錄水平及蛋白水平在AGA組(0.012 6±0.005 3和0.017 6±0.007 8)和NAGA組(0.009 5±0.003 6和0.014 0±0.004 1)均顯著高于健康對照組(0.004 2±0.001 2和0.005 8±0.0019,F=10.818,F=8.869,P均＜0.01).②在AGA組中TRF2轉錄水平均與淋巴細胞(LY)呈正相關(r=0.289,P＜0.05),TRF1轉錄水平與TRF2(r=0.935,P＜0.01)、LY(r=0.256,P＜0.05)、TG (r=0.281,P＜0.05)、極低密度脂蛋白(VLDL-C)(r=0.291,P＜0.05)均呈正相關.結論 耑TRF1、TRF2在原髮性GA患者的異常錶達及與LY呈正相關,提示其可能參與瞭痛風的髮生髮展及免疫與炎癥調節.
목적 탐토단립중복서렬결합단백(TRF)1화TRF2재원발성통풍성관절염(GA)발병중가능적작용.방법 장연구대상분위급성기GA(AGA)조77례、비급성기(NAGA)조49례급건강대조조79명.실시형광정량다취합매련반응(RT-qPCR)급단백질인적법분별검측TRF1화TRF2재3조PBMCs중전록수평급단백수평적표체.채용단인소방차분석,LSD법량량비교화Spearman상관분석행통계학처리.결과 ①TRF1、TRF2전록수평급단백수평재AGA조(0.012 6±0.005 3화0.017 6±0.007 8)화NAGA조(0.009 5±0.003 6화0.014 0±0.004 1)균현저고우건강대조조(0.004 2±0.001 2화0.005 8±0.0019,F=10.818,F=8.869,P균＜0.01).②재AGA조중TRF2전록수평균여림파세포(LY)정정상관(r=0.289,P＜0.05),TRF1전록수평여TRF2(r=0.935,P＜0.01)、LY(r=0.256,P＜0.05)、TG (r=0.281,P＜0.05)、겁저밀도지단백(VLDL-C)(r=0.291,P＜0.05)균정정상관.결론 단TRF1、TRF2재원발성GA환자적이상표체급여LY정정상관,제시기가능삼여료통풍적발생발전급면역여염증조절.
Objective To investigate the expression levels of telomeric protein telomeric repeat binding protein (TRF) 1 and TRF2 mRNA and protein in the peripheral blood mononuclear cells (PBMCs) of patients with primairy Gouty arthritis (GA),and the relations between these gene expression levels and disease activity were explored.Methods ① One hundred and twenty-six GA and 79 healthy controls (HC) were enrolled into the study.The clinical data of primary gouty arthritis patients and healthy controls were collected.PBMCs were isolated from blood in vitro.② Real-time quantitative polymerase chain reaction (RT-qPCR) was employed to test the expression of TRF1 and TRF2 mRNA in PBMCs from 77 AGA,49 NAGA and 79 HC,and the differences of gene expression in three groups were compared.③ TRF1 and TRF2 protein were measured by using Western blotting in PBMCs from 15 GA,9 NAGA and 12 healthy controls,and other protein expression in three groups were compared.④ Analysis of covariance,LSD method,and Spearman correlations were used for statistical analysis.Results The expression levels of TRF1 and TRF2 mRNA and protein in the PBMCs of AGA (0.012 6±0.005 3 vs 0.017 6±0.007 8) and NAGA (0.009 5±0.003 6 vs 0.014 0±0.004 1) patients was significantly increased compared to healthy individuals (0.004 2±0.001 2 vs 0.005 8± 0.001 9,F=10.818,F=8.869,P＜0.01).The expression level of TRF2 was correlated with LY (r=0.289,P＜ 0.05).The expression levels of TRF1 was correlated with TRF2 (r=0.935,P＜0.01),LY (r=0.256,P＜0.05),TG (r=0.281,P＜0.05),VLDL-C (r=0.291,P＜0.05).Conclusion TRF1 and TRF2 play a key role in the inflammatory immune regulation of GA,and they are involved in the pathogenesis of gouty immune and inflammatory responses.Further study is needed to clarify the expression and functions of telomeric proteins.
