中华航空航天医学杂志
中華航空航天醫學雜誌
중화항공항천의학잡지
CHINESE JOURNAL OF AEROSPACE MEDICINE
2014年
3期
194-197,封4
,共5页
朱敏立%王萍%崔彦%裴士杰%王小辉
硃敏立%王萍%崔彥%裴士傑%王小輝
주민립%왕평%최언%배사걸%왕소휘
失重模拟%链球菌,肺炎%模型,动物%大鼠,Wistar
失重模擬%鏈毬菌,肺炎%模型,動物%大鼠,Wistar
실중모의%련구균,폐염%모형,동물%대서,Wistar
Weightlessness simulation%Streptococcus pneumoniae%Models,animal%Rats,Wistar
目的 建立模拟失重条件下大鼠细菌性肺炎的动物模型,观察模拟失重对大鼠肺组织肺炎链球菌感染的影响. 方法 36只雄性Wistar大鼠按随机数字表法分为失重感染(weightless and infection,WI)组、失重对照(weightlessness and control,WC)组和1G重力感染(1 G gravity and infection,GI)组,每组12只.实验第8天(注入细菌后的第6天)处死各组大鼠,检测血常规、C-反应蛋白(C-reactive protein,CRP)及CD4 T淋巴细胞和CD8 T淋巴细胞的比值(CD4+/CD8+).观察大鼠肺组织病理变化. 结果 各组大鼠白细胞计数差异无统计学意义,中性粒细胞(neutrophils,NEU)计数及NEU比值差异有统计学意义(F=6.12、4.23,P＜0.01、＜0.05),其中WI组最高;3组大鼠的CRP差异有统计学意义(F=19.49,P＜0.01),其中WI组最高,WC组最低;各组大鼠外周血中CD4+/CD8+差异无统计学意义.WI组肺组织病理改变最严重,炎症反应较重;GI组可见部分肺组织病理改变,炎症反应程度较轻;WC组肺组织病理改变和炎症反应均最轻. 结论 模拟失重(微重力)状态下大鼠机体免疫功能降低,肺炎链球菌肺部感染的病理改变严重,炎症反应较重.
目的 建立模擬失重條件下大鼠細菌性肺炎的動物模型,觀察模擬失重對大鼠肺組織肺炎鏈毬菌感染的影響. 方法 36隻雄性Wistar大鼠按隨機數字錶法分為失重感染(weightless and infection,WI)組、失重對照(weightlessness and control,WC)組和1G重力感染(1 G gravity and infection,GI)組,每組12隻.實驗第8天(註入細菌後的第6天)處死各組大鼠,檢測血常規、C-反應蛋白(C-reactive protein,CRP)及CD4 T淋巴細胞和CD8 T淋巴細胞的比值(CD4+/CD8+).觀察大鼠肺組織病理變化. 結果 各組大鼠白細胞計數差異無統計學意義,中性粒細胞(neutrophils,NEU)計數及NEU比值差異有統計學意義(F=6.12、4.23,P＜0.01、＜0.05),其中WI組最高;3組大鼠的CRP差異有統計學意義(F=19.49,P＜0.01),其中WI組最高,WC組最低;各組大鼠外週血中CD4+/CD8+差異無統計學意義.WI組肺組織病理改變最嚴重,炎癥反應較重;GI組可見部分肺組織病理改變,炎癥反應程度較輕;WC組肺組織病理改變和炎癥反應均最輕. 結論 模擬失重(微重力)狀態下大鼠機體免疫功能降低,肺炎鏈毬菌肺部感染的病理改變嚴重,炎癥反應較重.
목적 건립모의실중조건하대서세균성폐염적동물모형,관찰모의실중대대서폐조직폐염련구균감염적영향. 방법 36지웅성Wistar대서안수궤수자표법분위실중감염(weightless and infection,WI)조、실중대조(weightlessness and control,WC)조화1G중력감염(1 G gravity and infection,GI)조,매조12지.실험제8천(주입세균후적제6천)처사각조대서,검측혈상규、C-반응단백(C-reactive protein,CRP)급CD4 T림파세포화CD8 T림파세포적비치(CD4+/CD8+).관찰대서폐조직병리변화. 결과 각조대서백세포계수차이무통계학의의,중성립세포(neutrophils,NEU)계수급NEU비치차이유통계학의의(F=6.12、4.23,P＜0.01、＜0.05),기중WI조최고;3조대서적CRP차이유통계학의의(F=19.49,P＜0.01),기중WI조최고,WC조최저;각조대서외주혈중CD4+/CD8+차이무통계학의의.WI조폐조직병리개변최엄중,염증반응교중;GI조가견부분폐조직병리개변,염증반응정도교경;WC조폐조직병리개변화염증반응균최경. 결론 모의실중(미중력)상태하대서궤체면역공능강저,폐염련구균폐부감염적병리개변엄중,염증반응교중.
Objective To establish bacterial pneumonia model of rats in simulated weightlessness state,and to observe the effects of weightless on streptococcus pneumoniae infection to the lung tissue of rats.Methods Thirty-six male Westar rats were randomly divided into weightlessness and infection (WI) group (n=12),weightlessness and control (WC) group (n=12),and 1 G gravity and infection (GI) group (n=12).On the 8th day after bacterial suspension instilled into trachea,the rats were killed.The leukocytes and neutrophils,C-reactive protein level,CD4+/CD8+ ratios in the peripheral blood were analyzed.The pathology of lung tissue was also observed.Results There were statistical significances of neutrophilic granulocyte count (NEU),neutrophilic granulocyte percentage (NEU％) among three groups (F=6.12,P＜0.01 and F=4.23,P＜0.05 respectively),and the WI group showed the highest values but no statistical difference was found in leukocytes count.The WI group had the highest level of CRP while the WC group had the lowest,and statistical significances were seen among three groups (F=19.49,P＜0.01).But CD4+/CD8+ ratios in peripheral blood among three groups had no statistical differences.The changes of lung pathology in WI group were the most severe.Some of these changes were also visible in WC group and GI group,but the inflammatory response to a lesser extent,and the pathological changes were the least in WC group.Conclusions The immune function of rat would reduce in weightlessness environment.Once the lung was insulted by streptococcus pneumoniae,the pathological changes and inflammatory response become more severe.
