中华结核和呼吸杂志
中華結覈和呼吸雜誌
중화결핵화호흡잡지
Chinese Journal of Tuberculosis and Respiratory Diseases
2014年
12期
888-892
,共5页
姜秀峰%苏梅%丁文筱%丁宁%黄茂%张希龙
薑秀峰%囌梅%丁文篠%丁寧%黃茂%張希龍
강수봉%소매%정문소%정저%황무%장희룡
间歇低氧%睡眠呼吸暂停,阻塞性%脂联素
間歇低氧%睡眠呼吸暫停,阻塞性%脂聯素
간헐저양%수면호흡잠정,조새성%지련소
Intermittent hypoxia%Sleep apnea obstructive%Adiponectin
目的 观察慢性间歇低氧(CIH)对大鼠心功能及血压的影响,探讨脂联素对其保护作用.方法 通过随机数字表法将24只Wistar雄性大鼠分为3组,每组8只,分别为常氧对照组(对照组)、CIH组和CIH-脂联素干预组(干预组).对照组呼吸正常空气,CIH组和干预组每天行8 h CIH,干预组每周第1天经尾静脉注射脂联素20 μg.比较各组大鼠的超声心动图,血压,血浆脂联素、内皮素-1、内皮型一氧化氮合酶(eNOS)的差异.28 d后处死大鼠,观察各组大鼠心肌细胞结构的改变.结果 与对照组相比,CIH组大鼠心脏与体重比值显著增加(0.070±0.008和0.057±0.009,P＜0.05),血压显著升高[(132±4)和(123±6)mmHg,l mmHg=0.133 kPa,P＜0.05],左心室射血分数明显降低[(70.3±4.1)％、(84.1±2.5)％,P＜0.05].血浆内皮素-1水平均升高[(26.2±6.9)和(7.7±2.7)ng/L,P＜0.05],NO;/N03[(37±9)和(65±10) μmol/L,P＜0.05]和eNOS水平降低[(18±5)、(27 ±6) μg/L,P＜0.05].干预组大鼠心脏与体重比值,血压,左心室射血分数较CIH组均有所改善,但尚未达到对照组水平.干预组大鼠血浆内皮素-1水平尽管高于对照组,但与CIH组比较差异无统计学意义;NO2-/NO3-水平[(55±10) μmol/L]明显高于CIH组[(37±9) μmol/L,P＜0.05],与CIH组比较差异无统计学意义.相关性分析显示NO2-/NO3-和eNOS水平与收缩压呈负相关;心脏与体重比值、LVED、内皮素-1、NO2-/NO3-与LVEF呈负相关.病理检查结果显示CIH组大鼠心肌细胞损伤明显,细胞肿胀、排列紊乱.结论 CIH可造成大鼠心功能损伤,这种损伤可能通过氧化应激引起血清内皮素-1、NO含量失衡,导致内皮功能障碍,进而损伤心肌有关.而脂联素可能通过减轻氧化应激损伤,改善血管内皮功能,从而减轻间歇低氧造成的心脏损伤.
目的 觀察慢性間歇低氧(CIH)對大鼠心功能及血壓的影響,探討脂聯素對其保護作用.方法 通過隨機數字錶法將24隻Wistar雄性大鼠分為3組,每組8隻,分彆為常氧對照組(對照組)、CIH組和CIH-脂聯素榦預組(榦預組).對照組呼吸正常空氣,CIH組和榦預組每天行8 h CIH,榦預組每週第1天經尾靜脈註射脂聯素20 μg.比較各組大鼠的超聲心動圖,血壓,血漿脂聯素、內皮素-1、內皮型一氧化氮閤酶(eNOS)的差異.28 d後處死大鼠,觀察各組大鼠心肌細胞結構的改變.結果 與對照組相比,CIH組大鼠心髒與體重比值顯著增加(0.070±0.008和0.057±0.009,P＜0.05),血壓顯著升高[(132±4)和(123±6)mmHg,l mmHg=0.133 kPa,P＜0.05],左心室射血分數明顯降低[(70.3±4.1)％、(84.1±2.5)％,P＜0.05].血漿內皮素-1水平均升高[(26.2±6.9)和(7.7±2.7)ng/L,P＜0.05],NO;/N03[(37±9)和(65±10) μmol/L,P＜0.05]和eNOS水平降低[(18±5)、(27 ±6) μg/L,P＜0.05].榦預組大鼠心髒與體重比值,血壓,左心室射血分數較CIH組均有所改善,但尚未達到對照組水平.榦預組大鼠血漿內皮素-1水平儘管高于對照組,但與CIH組比較差異無統計學意義;NO2-/NO3-水平[(55±10) μmol/L]明顯高于CIH組[(37±9) μmol/L,P＜0.05],與CIH組比較差異無統計學意義.相關性分析顯示NO2-/NO3-和eNOS水平與收縮壓呈負相關;心髒與體重比值、LVED、內皮素-1、NO2-/NO3-與LVEF呈負相關.病理檢查結果顯示CIH組大鼠心肌細胞損傷明顯,細胞腫脹、排列紊亂.結論 CIH可造成大鼠心功能損傷,這種損傷可能通過氧化應激引起血清內皮素-1、NO含量失衡,導緻內皮功能障礙,進而損傷心肌有關.而脂聯素可能通過減輕氧化應激損傷,改善血管內皮功能,從而減輕間歇低氧造成的心髒損傷.
목적 관찰만성간헐저양(CIH)대대서심공능급혈압적영향,탐토지련소대기보호작용.방법 통과수궤수자표법장24지Wistar웅성대서분위3조,매조8지,분별위상양대조조(대조조)、CIH조화CIH-지련소간예조(간예조).대조조호흡정상공기,CIH조화간예조매천행8 h CIH,간예조매주제1천경미정맥주사지련소20 μg.비교각조대서적초성심동도,혈압,혈장지련소、내피소-1、내피형일양화담합매(eNOS)적차이.28 d후처사대서,관찰각조대서심기세포결구적개변.결과 여대조조상비,CIH조대서심장여체중비치현저증가(0.070±0.008화0.057±0.009,P＜0.05),혈압현저승고[(132±4)화(123±6)mmHg,l mmHg=0.133 kPa,P＜0.05],좌심실사혈분수명현강저[(70.3±4.1)％、(84.1±2.5)％,P＜0.05].혈장내피소-1수평균승고[(26.2±6.9)화(7.7±2.7)ng/L,P＜0.05],NO;/N03[(37±9)화(65±10) μmol/L,P＜0.05]화eNOS수평강저[(18±5)、(27 ±6) μg/L,P＜0.05].간예조대서심장여체중비치,혈압,좌심실사혈분수교CIH조균유소개선,단상미체도대조조수평.간예조대서혈장내피소-1수평진관고우대조조,단여CIH조비교차이무통계학의의;NO2-/NO3-수평[(55±10) μmol/L]명현고우CIH조[(37±9) μmol/L,P＜0.05],여CIH조비교차이무통계학의의.상관성분석현시NO2-/NO3-화eNOS수평여수축압정부상관;심장여체중비치、LVED、내피소-1、NO2-/NO3-여LVEF정부상관.병리검사결과현시CIH조대서심기세포손상명현,세포종창、배렬문란.결론 CIH가조성대서심공능손상,저충손상가능통과양화응격인기혈청내피소-1、NO함량실형,도치내피공능장애,진이손상심기유관.이지련소가능통과감경양화응격손상,개선혈관내피공능,종이감경간헐저양조성적심장손상.
Objective To observe the effect of chronic intermittent hypoxia (CIH) on rat cardiac function and blood pressure,and the protective role of adiponectin (Ad).Methods A total of 24 male Wistar rats were randomly and equally divided into 3 groups:normal control group (NC group),chronic intermittent hypoxygen group (CIH group) and CIH + Ad group.Normal air breathing for NC group and CHI for CIH and CIH + Ad groups were conducted for 28 days.In addition,rats in CIH + Ad group were given intravenous adiponectin at a dosage of 20 μg each time,once a week for successive 4 weeks.The results of echocardiography,blood pressure,plasma adiponectin,endothelin-1 (ET-1) and endothelial nitric oxide synthase (eNOS) were compared among the three groups after 28 days.Results HE stain showed that the myocardial cells of CIH rats were damaged by CIH.Compared with NC group,rats in CIH group presented with a greater heart/body weight ratio (0.070 ± 0.008 vs.0.057 ± 0.009,P ＜ 0.05) and systolic blood pressure [(132±4) vs.(123 ±6) mmHg(1 mmHg =0.133 kPa),P＜0.05] and a lower LVEF [(70.3±4.1)％ vs.(84.1 ±2.5)％,P＜0.05].Plasma ET-1 was increased while NO2-/NO3-and eNOS decreased in CIH group,compared with NC group [(26.2 ± 6.9) ng/L vs.(7.7 ± 2.7) ng/L,(37 ±9) μmol/L vs.(65 ± 10) μmol/L,(18 ±5) μg/L vs.(27 ±6) μg/L,respectively; P ＜0.05].The heart/body weight ratio,blood pressure and LVEF were improved in CIH + Ad group compared with those in CIH group [0.064 ± 0.009 vs.0.070 ± 0.008,(127 ± 6) mmHg vs.(132 ± 4) mmHg,P ＞ 0.05; (79 ±7)％ vs.(70 ±4)％,P ＜0.05; respectively].Plasma ET-1 levels in CIH + Ad and CIH groups showed no significant difference,but were significantly lower in NC group.However,rats in CIH + Ad group had a higher NO2-/NO3-level than that in CIH group.Bivariate Correlations showed that NO2-/NO3-and eNOS were negatively correlated with systolic blood pressure while heart/body weight ratio,LVEDs,ET-1 and NO2-/NO3-were negatively correlated with left ventricular ejection fraction.Conclusions Through xidative stress,ET-1 and NO imbalance and impaired vascular endothelial function,cardiac function could be damaged by CIH in rats,while supplement of extrinsic adiponectin could improve these damages.
