CAJ | 학술논문

目的探讨皮肤黑素瘤BRAF V600E突变蛋白表达情况,分析免疫组化法检测V600E突变的灵敏度和特异度.方法应用抗BRAF V600E单克隆抗体的免疫组化法检测103例皮肤黑素瘤、40例色素痣石蜡包埋组织切片中BRAF V600E突变蛋白表达水平.采用SPSS 17.0统计软件进行统计分析,率的比较采用x2检验.结果 BRAF V600E突变蛋白阳性表达率在皮肤黑素瘤中为20.4％(21/103),色素痣中为5.0％(2/40),两组差异有统计学意义(x2=5.06,P＜ 0.05).黑素瘤BRAF V600E突变蛋白的表达率在不同年龄组[＜60岁组表达率为29.8％(14/47),≥60岁组为12.5％(7/56)]、不同民族[维吾尔族组为30.2％(13/43),汉族组为13.3％(8/60)]、不同发病部位[肢端为13.6％(6/42)、黏膜为11.8％ (4/29)、非肢端为45.8％(11/32)]、不同Clark分级[Ⅰ～Ⅲ级组为8.6％(4/42),Ⅳ～Ⅴ级组为12.4％(17/61)]组间表达差异均有统计学意义(P＜0.05),而在不同性别、有无淋巴结转移组间表达差异均无统计学意义(P＞0.05).免疫组化检测恶性黑素瘤中BRAF V600E突变灵敏度为100％(15/15),特异度为98.5％(65/66).结论 BRAF V600E突变蛋白在皮肤黑素瘤中高表达,在维吾尔族人群表达率高于汉族人群;免疫组化法检测BRAF V600E突变具有准确、快速等特点.
목적 탐토피부흑소류BRAF V600E돌변단백표체정황,분석면역조화법검측V600E돌변적령민도화특이도.방법 응용항BRAF V600E단극륭항체적면역조화법검측103례피부흑소류、40례색소지석사포매조직절편중BRAF V600E돌변단백표체수평.채용SPSS 17.0통계연건진행통계분석,솔적비교채용x2검험.결과 BRAF V600E돌변단백양성표체솔재피부흑소류중위20.4％(21/103),색소지중위5.0％(2/40),량조차이유통계학의의(x2=5.06,P＜ 0.05).흑소류BRAF V600E돌변단백적표체솔재불동년령조[＜60세조표체솔위29.8％(14/47),≥60세조위12.5％(7/56)]、불동민족[유오이족조위30.2％(13/43),한족조위13.3％(8/60)]、불동발병부위[지단위13.6％(6/42)、점막위11.8％ (4/29)、비지단위45.8％(11/32)]、불동Clark분급[Ⅰ～Ⅲ급조위8.6％(4/42),Ⅳ～Ⅴ급조위12.4％(17/61)]조간표체차이균유통계학의의(P＜0.05),이재불동성별、유무림파결전이조간표체차이균무통계학의의(P＞0.05).면역조화검측악성흑소류중BRAF V600E돌변령민도위100％(15/15),특이도위98.5％(65/66).결론 BRAF V600E돌변단백재피부흑소류중고표체,재유오이족인군표체솔고우한족인군;면역조화법검측BRAF V600E돌변구유준학、쾌속등특점.
Objective To detect the expression of BRAF V600E mutant protein in cutaneous malignant melanoma (CMM),and to evaluate the sensitivity and specificity of immunohistochemistry (IHC) in detecting BRAF V600E mutation.Methods IHC with an anti-BRAF V600E monoclonal antibody was performed to detect the expression of BRAF V600E mutant protein in paraffin-embedded tissue sections from 103 patients with CMM and 40 patients with nevus.Statistical analysis was carried out with SPSS software version 17.0,and the expression rate of BRAF V600E mutant protein was compared by chi-square test.Results The expression rate of BRAF V600E mutant protein in the CMM patients was 20.4％ (21/103),significantly higher than that in the nevus patients (5.0％ (2/40),x2 =5.06,P ＜ 0.05).Significant differences were observed in the expression rate of BRAF V600E mutant protein between CMM patients of different age groups (29.8％ (14/47) in patients aged ＜ 60 years vs.12.5％ (7/56) in those aged ≥ 60 years,P ＜ 0.05) and nationality (30.2％ (13/43) for Uygur nationality vs.13.3％ (8/60) for Han nationality,P ＜ 0.05),as well as among CMM lesions from different anatomical sites (13.6％ (6/42) in acral sites vs.11.8％ (4/29) in mucous membrane vs.45.8％ (11/32) in non-acral sites,P ＜ 0.05) and of different Clark levels (8.6％ (4/42) for grade Ⅰ-Ⅲ vs.12.4％ (17/61) for grade Ⅳ-Ⅴ,P＜ 0.05),but not between male and female CMM patients or between CMM patients with lymph node metastasis and those without (both P ＞ 0.05).IHC with the anti-BRAF V600E antibody showed a sensitivity of 100％ (15/15) and a specificity of 98.5％ (65/66) in detecting BRAF V600E mutation.Conclusions The expression of BRAF V600E mutant protein is up-regulated in CMM lesions,and CMM patients of Uygur nationality seems to have a higher expression rate than those of Han nationality.IHC appears to be an accurate and rapid method to detect V600E BRAF mutation.