中华器官移植杂志
中華器官移植雜誌
중화기관이식잡지
CHINESE JOURNAL OF ORGAN TRANSPLANTATION
2014年
10期
626-630
,共5页
郑圣斌%施晓雷%任昊桢%韩冰%吴亚夫%徐素琴%陈骏%丁义涛
鄭聖斌%施曉雷%任昊楨%韓冰%吳亞伕%徐素琴%陳駿%丁義濤
정골빈%시효뢰%임호정%한빙%오아부%서소금%진준%정의도
白细胞介素10%转化生长因子-β1%间充质干细胞%肝移植%急性排斥反应
白細胞介素10%轉化生長因子-β1%間充質榦細胞%肝移植%急性排斥反應
백세포개소10%전화생장인자-β1%간충질간세포%간이식%급성배척반응
Interleukin-10%Transforming growth factor-β1%Mesenchymal stem cells%Liver transplantation%Acute rejection
目的 探讨白细胞介素-10(ID-10)和转化生长因子-β1 (TGF-β1)基因共修饰骨髓间充质干细胞(MSC)对大鼠肝移植急性排斥反应的影响.方法 近交系大鼠DA为供鼠,Lewis为受鼠,建立同种异体原位肝移植,随机数字表法均分为6组,每组20只:空白组,移植前5d受鼠腹腔输注生理盐水1 ml;共转染组,移植前5d受鼠输注IL-10和TGF-β1基因共修饰MSC(5×106/ml)1 ml;MSC组,移植前5d受鼠输注MSC(5×106/ml)1 ml; IL-10-MSC组,移植前5d受鼠输注IL-10基因修饰MSC(5×106/ml)1 ml; TGF-β1-MSC组,移植前5d受鼠输注TGF-β1基因修饰MSC(5×106/ml)1 ml;空质粒组,移植前5d受鼠输注空质粒转染的MSC(5×106/ml)1 ml.各组于术后观察一般情况,及肝功能、血清细胞因子、肝脏组织病理和受鼠生存率.结果 术后7d,共转染组丙氨酸转氨酶(ALT)和胆红素总量(TBil)明显低于其他5组,空白组ALT和TBil高于其他5组,差异有统计学意义(P＜0.05).肝脏组织病理检查结果提示,术后7d共转染组无排斥反应,MSC组和空质粒组发生轻-中度排斥反应;IL-10-MSC组和TGF-β1-MSC组发生轻度排斥反应;空白组发生重度排斥反应.共转染组大鼠的生存时间为(49.7±4.05)d,较其他5组明显延长,差异有统计学意义(P＜0.05).结论 IL-10和TGF-β1修饰的MSC能明显减轻大鼠同种异体肝移植后急性排斥反应,延长受者存活时间.
目的 探討白細胞介素-10(ID-10)和轉化生長因子-β1 (TGF-β1)基因共脩飾骨髓間充質榦細胞(MSC)對大鼠肝移植急性排斥反應的影響.方法 近交繫大鼠DA為供鼠,Lewis為受鼠,建立同種異體原位肝移植,隨機數字錶法均分為6組,每組20隻:空白組,移植前5d受鼠腹腔輸註生理鹽水1 ml;共轉染組,移植前5d受鼠輸註IL-10和TGF-β1基因共脩飾MSC(5×106/ml)1 ml;MSC組,移植前5d受鼠輸註MSC(5×106/ml)1 ml; IL-10-MSC組,移植前5d受鼠輸註IL-10基因脩飾MSC(5×106/ml)1 ml; TGF-β1-MSC組,移植前5d受鼠輸註TGF-β1基因脩飾MSC(5×106/ml)1 ml;空質粒組,移植前5d受鼠輸註空質粒轉染的MSC(5×106/ml)1 ml.各組于術後觀察一般情況,及肝功能、血清細胞因子、肝髒組織病理和受鼠生存率.結果 術後7d,共轉染組丙氨痠轉氨酶(ALT)和膽紅素總量(TBil)明顯低于其他5組,空白組ALT和TBil高于其他5組,差異有統計學意義(P＜0.05).肝髒組織病理檢查結果提示,術後7d共轉染組無排斥反應,MSC組和空質粒組髮生輕-中度排斥反應;IL-10-MSC組和TGF-β1-MSC組髮生輕度排斥反應;空白組髮生重度排斥反應.共轉染組大鼠的生存時間為(49.7±4.05)d,較其他5組明顯延長,差異有統計學意義(P＜0.05).結論 IL-10和TGF-β1脩飾的MSC能明顯減輕大鼠同種異體肝移植後急性排斥反應,延長受者存活時間.
목적 탐토백세포개소-10(ID-10)화전화생장인자-β1 (TGF-β1)기인공수식골수간충질간세포(MSC)대대서간이식급성배척반응적영향.방법 근교계대서DA위공서,Lewis위수서,건립동충이체원위간이식,수궤수자표법균분위6조,매조20지:공백조,이식전5d수서복강수주생리염수1 ml;공전염조,이식전5d수서수주IL-10화TGF-β1기인공수식MSC(5×106/ml)1 ml;MSC조,이식전5d수서수주MSC(5×106/ml)1 ml; IL-10-MSC조,이식전5d수서수주IL-10기인수식MSC(5×106/ml)1 ml; TGF-β1-MSC조,이식전5d수서수주TGF-β1기인수식MSC(5×106/ml)1 ml;공질립조,이식전5d수서수주공질립전염적MSC(5×106/ml)1 ml.각조우술후관찰일반정황,급간공능、혈청세포인자、간장조직병리화수서생존솔.결과 술후7d,공전염조병안산전안매(ALT)화담홍소총량(TBil)명현저우기타5조,공백조ALT화TBil고우기타5조,차이유통계학의의(P＜0.05).간장조직병리검사결과제시,술후7d공전염조무배척반응,MSC조화공질립조발생경-중도배척반응;IL-10-MSC조화TGF-β1-MSC조발생경도배척반응;공백조발생중도배척반응.공전염조대서적생존시간위(49.7±4.05)d,교기타5조명현연장,차이유통계학의의(P＜0.05).결론 IL-10화TGF-β1수식적MSC능명현감경대서동충이체간이식후급성배척반응,연장수자존활시간.
Objective To evaluate the effect and mechanism of mesenchymal stem cells modified by human interleukin-10 (IL-10) and transforming growth factor beta-1 gene (TGF-β1) on acute rejection in rat liver transplantation.Method One hundred and twenty cases of rat allogeneic orthotopic liver transplantation were accomplished in the strain combination of DA to Lewis in bred rats,and divided equally into 6 groups,including IL-10 and TGF-β1 cotransfection group,MSCs group,IL-10-MSCs group,TGF-β1-MSCs group,empty vector group,and control group.Five days before transplantation,5 × 10-6/mL cells were respectively injected to Lewis rats with distinct cells,such as,IL-10 and TGF-β1 cotransfected MSCs,MSCs,IL-10 transfected MSCs,TGF-β1 transfected MSCs,empty vector-transfected MSCs,or normal saline.Survival analysis was performed for 10 rats.The liver function [alanine aminotransferase (ALT) and total bilirubin (TBIL)] and cytokines alteration were evaluated in peripheral blood on the day 3,7 and 10 after transplantation.The pathological changes of the liver graft were observed.Result At day 7,ALT and TBIL levels in cotransfection group was reverted to the normal,and those in control group were obviously higher than in other groups (P＜0.05).At day 7,histologic results showed that acute severe rejection was observed in control group,and no rejection was seen in cotransfection group.Mild rejection occurred in IL-10-MSCs group and TGF-β1-MSCs group,and mild to moderate rejection in MSCs group and empty vector group.The survival of cotransfection group was significantly longer than that of other five groups with the difference being statistically significant (P＜0.05).Conclusion MSCs modified by IL-10 and TGF-β1 could inhibit acute allograft rejection and prolong the survival time of the recipients.
