CAJ | 학술논문

目的探讨载脂蛋白E (ApoE)基因多态性与脑梗死发生部位及梗死灶体积的相关性. 方法采用病例-对照研究设计,对自2011年9月至2012年11月泸州医学院附属医院神经内、外科收治的162例脑梗死患者(脑梗死组)和同期体检的120例健康人(对照组)的血样采用多重聚合酶连接反应-连接酶检测反应分型法(PCR-LDR)测定ApoE基因的多态性,分析不同脑梗死部位ApoE基因型分布及各等位基因频率的差异,以及各基因型与梗死灶体积的关系. 结果脑梗死组和对照组之间ApoE基因型分布和等位基因频率比较差异均无统计学意义(P＞0.05).各脑梗死部位间ApoE基因型分布和等位基因频率比较差异均无统计学意义(P＞0.05).脑梗死组中E4/X基因型(E4/3基因型和E4/4基因型合并)的平均梗死灶体积[(15.7±2.3) cm3]与E3/3基因型[(10.2±2.1) cm3]及E2/X基因型(E2/2基因型和E2/3基因型合并)[(9.5±1.8)cm3]比较差异具有统计学意义(P＜0.05),且E4/X基因型中大面积脑梗死比例(56％)较E3/3基因型(28.1％)及E2/X基因型(26.3％)更高,差异具有统计学意义(P＜0.05). 结论 ApoE基因多态性与脑梗死发生部位无相关性,而与梗死灶体积相关,其中携E4等位基因者较易发生大面积脑梗死.
목적 탐토재지단백E (ApoE)기인다태성여뇌경사발생부위급경사조체적적상관성. 방법 채용병례-대조연구설계,대자2011년9월지2012년11월로주의학원부속의원신경내、외과수치적162례뇌경사환자(뇌경사조)화동기체검적120례건강인(대조조)적혈양채용다중취합매련접반응-련접매검측반응분형법(PCR-LDR)측정ApoE기인적다태성,분석불동뇌경사부위ApoE기인형분포급각등위기인빈솔적차이,이급각기인형여경사조체적적관계. 결과 뇌경사조화대조조지간ApoE기인형분포화등위기인빈솔비교차이균무통계학의의(P＞0.05).각뇌경사부위간ApoE기인형분포화등위기인빈솔비교차이균무통계학의의(P＞0.05).뇌경사조중E4/X기인형(E4/3기인형화E4/4기인형합병)적평균경사조체적[(15.7±2.3) cm3]여E3/3기인형[(10.2±2.1) cm3]급E2/X기인형(E2/2기인형화E2/3기인형합병)[(9.5±1.8)cm3]비교차이구유통계학의의(P＜0.05),차E4/X기인형중대면적뇌경사비례(56％)교E3/3기인형(28.1％)급E2/X기인형(26.3％)경고,차이구유통계학의의(P＜0.05). 결론 ApoE기인다태성여뇌경사발생부위무상관성,이여경사조체적상관,기중휴E4등위기인자교역발생대면적뇌경사.
Objective To investigate the relationships between the apolipoprotein E (ApoE) gene polymorphisms and both the location and size of cerebral infarction.Methods A case-control study was chosen; 162 cerebral infarction patients,admitted to our hospital from September 2011 to November 2012,and 120 normal controls were chosen; their blood samples were detected by multiple polymerase chain reaction-ligase detection reaction (PCR-LDR) to analyze the ApoE gene polymorphisms.Its differences of genotype distribution or allele frequencies in different locations of cerebral infarction were analyzed,and the relationships between allele frequencies and volume of cerebral infarction was analyzed.Results No significant difference was found either in ApoE genotype distributions or allele frenquencies between the cerebral infarction patients and controls (P＞0.05); the ApoE genotype distributions or allele frenquencies showed no significant difference among patients having different locations of cerebral infarction (P＞0.05).A significant difference was observed in the average infarction volume between E4/X carriers (the E4/3 and E4/4 genotype combination,[15.7±2.3] cm3) and E3/3 carries ([10.2±2.1] cm3) and E2/X carriers (the E2/2 and E2/3 genotype combination,[9.5± 1.8] cm3,P＜ 0.05); and the ratio of massive cerebral infarction of E4/X carriers (56％) was significantly higher than E3/3 or E2/X carriers (26.3％,P＜0.05).Conclusions There is no correlation between ApoE genotype polymorphisms and cerebral infarction location; however it may be associated with infarction volume.The E4/X carriers are more prone to massive cerebral infarction,and its volume is larger than that of E2 and E3.