CAJ | 학술논문

目的探讨核干因子(NS)和p53上调凋亡因子(PUMA)蛋白在人脑胶质瘤中的表达及其临床意义. 方法收集郑州大学第五附属医院神经外科自2011年1月至2013年2月手术切除并经病理证实的脑胶质瘤标本62例,其中WHO分级Ⅰ级8例、Ⅱ级21例,Ⅲ级19例,Ⅳ级14例.另取同期10例因颅脑损伤行内减压术获取的的正常脑组织作为对照组,免疫组化染色检测标本中NS和PUMA蛋白的表达. 结果胶质瘤组织NS蛋白的表达明显高于对照组,胶质瘤组织PUMA蛋白的表达明显低于对照组,差异均有统计学意义(P＜0.05);与Ⅰ+Ⅱ级胶质瘤比较,Ⅲ+Ⅳ级胶质瘤组织NS蛋白的表达较高(0.224±0.015vs 0.302±0.024),PUMA蛋白的表达较低(0.182±0.020vs 0.167±0.018),差异有统计学意义(P＜0.05). 结论 NS和PUMA蛋白与胶质瘤的恶性程度密切相关,二者在胶质瘤的发生和发展过程中起重要的作用.
목적 탐토핵간인자(NS)화p53상조조망인자(PUMA)단백재인뇌효질류중적표체급기림상의의. 방법 수집정주대학제오부속의원신경외과자2011년1월지2013년2월수술절제병경병리증실적뇌효질류표본62례,기중WHO분급Ⅰ급8례、Ⅱ급21례,Ⅲ급19례,Ⅳ급14례.령취동기10례인로뇌손상행내감압술획취적적정상뇌조직작위대조조,면역조화염색검측표본중NS화PUMA단백적표체. 결과 효질류조직NS단백적표체명현고우대조조,효질류조직PUMA단백적표체명현저우대조조,차이균유통계학의의(P＜0.05);여Ⅰ+Ⅱ급효질류비교,Ⅲ+Ⅳ급효질류조직NS단백적표체교고(0.224±0.015vs 0.302±0.024),PUMA단백적표체교저(0.182±0.020vs 0.167±0.018),차이유통계학의의(P＜0.05). 결론 NS화PUMA단백여효질류적악성정도밀절상관,이자재효질류적발생화발전과정중기중요적작용.
Objective To investigate the expressions ofnucleostemin (NS) and p53 up-regulated apoptotic factor (PUMA) proteins in human gliomas and its clinical significance.Methods Sixty-two human gliomas,collected in our hospital from January 2011 to February 2013 and confirmed by pathology,were used in our study; according to the WHO grading,grade Ⅰ included 8 patients in the study group,grade Ⅱ 21 patients,grade Ⅲ 19 patients and grade Ⅳ 14 patients.Other 10 patients accepted decompressions in our hospital at the same time period were used as control group.The expressions of NS and PUMA proteins in the brain tissues were detected by immunohistochemical S-P method.Results The NS protein expression was significantly higher and PUMA protein expression was statistically lower in study group than those in the control group (P＜0.05).As compared with gliomas of grade Ⅰ and Ⅱ,gliomas of grade Ⅲ and Ⅳ had significantly higher NS protein expression and lower PUMA protein expression (P＜0.05).Conclusions The expression changes of NS and PUMA are closely associated with gliomas malignant degrees,and the two play important roles in the progression and proliferation of glioma cells.