国际医药卫生导报
國際醫藥衛生導報
국제의약위생도보
INTERNATIONAL MEDICINE & HEALTH GUIDANCE NEWS
2014年
23期
3531-3534
,共4页
柳倩茹%黄绮云%池霖生%张静雯%朱莉
柳倩茹%黃綺雲%池霖生%張靜雯%硃莉
류천여%황기운%지림생%장정문%주리
玻璃化冷冻%慢速程序化冷冻%卵裂期胚胎%体外受精-胚胎移植
玻璃化冷凍%慢速程序化冷凍%卵裂期胚胎%體外受精-胚胎移植
파리화냉동%만속정서화냉동%란렬기배태%체외수정-배태이식
Vitrification%Slow-freezing sequencing%Cleaved embryo%In vitro fertilization and embryo transfer
目的 探讨玻璃化冷冻法对卵裂期冻融胚胎移植周期结局的影响.方法 将接受体外受精-胚胎移植患者剩余的可利用胚胎进行玻璃化冷冻或者慢速程序化冷冻,比较胚胎复苏后的复苏率、胚胎存活率、完整存活率、种植率、妊娠率等指标.结果 玻璃化冷冻复苏248个周期、746个胚胎,存活643个(86.2％),完整存活594个(79.4％),移植244个周期,临床妊娠107个周期(43.9％),种植率26.1％,流产9个周期(8.5％).慢速程序冷冻复苏225个周期、818个胚胎,存活598个(73.1％),完整存活436个(53.3％),移植225个周期,临床妊娠74个周期(32.9％),种植率15.2％,流产7个周期(9.5％).玻璃化法冷冻后的胚胎存活率、完整存活率、临床妊娠率、种植率明显高于慢速程序冷冻法(P＜0.05).结论 玻璃化冷冻法是冻存人类卵裂期胚胎理想的方法,可以提高胚胎的有效利用.
目的 探討玻璃化冷凍法對卵裂期凍融胚胎移植週期結跼的影響.方法 將接受體外受精-胚胎移植患者剩餘的可利用胚胎進行玻璃化冷凍或者慢速程序化冷凍,比較胚胎複囌後的複囌率、胚胎存活率、完整存活率、種植率、妊娠率等指標.結果 玻璃化冷凍複囌248箇週期、746箇胚胎,存活643箇(86.2％),完整存活594箇(79.4％),移植244箇週期,臨床妊娠107箇週期(43.9％),種植率26.1％,流產9箇週期(8.5％).慢速程序冷凍複囌225箇週期、818箇胚胎,存活598箇(73.1％),完整存活436箇(53.3％),移植225箇週期,臨床妊娠74箇週期(32.9％),種植率15.2％,流產7箇週期(9.5％).玻璃化法冷凍後的胚胎存活率、完整存活率、臨床妊娠率、種植率明顯高于慢速程序冷凍法(P＜0.05).結論 玻璃化冷凍法是凍存人類卵裂期胚胎理想的方法,可以提高胚胎的有效利用.
목적 탐토파리화냉동법대란렬기동융배태이식주기결국적영향.방법 장접수체외수정-배태이식환자잉여적가이용배태진행파리화냉동혹자만속정서화냉동,비교배태복소후적복소솔、배태존활솔、완정존활솔、충식솔、임신솔등지표.결과 파리화냉동복소248개주기、746개배태,존활643개(86.2％),완정존활594개(79.4％),이식244개주기,림상임신107개주기(43.9％),충식솔26.1％,유산9개주기(8.5％).만속정서냉동복소225개주기、818개배태,존활598개(73.1％),완정존활436개(53.3％),이식225개주기,림상임신74개주기(32.9％),충식솔15.2％,유산7개주기(9.5％).파리화법냉동후적배태존활솔、완정존활솔、림상임신솔、충식솔명현고우만속정서냉동법(P＜0.05).결론 파리화냉동법시동존인류란렬기배태이상적방법,가이제고배태적유효이용.
Objective To investigate the influence of vitrification method on clinical outcome of human frozen-thawed cleaved embryo transfer.Methods In the in vitro fertilization and embryo transfer cycles,supernumerary embryos were cryopreserved by vitrification or slow-freezing sequencing method.The embryo survival rate,intact survival rate,pregnant rate and implantation rate were compared after thawing.Results In 248 vitrified embryo thaw cycles,746 vitrified embryos were thawed,and 643(86.2％) of them survived and 594(79.4％) of them intactly survived after the thawing.In 244 women,632 embryos were transferred,and 107(43.9％) of them got clinical pregnancy,the implantation rate was 26.1％ (165/632).As to slow-freezing method,in 225 embryo thaw cycles,818 embryos were thawed,598 (73.1％) of them survived,436(53.3％) of them intactly survived,and 594 embryos were transferred in 225 cycles,and 74 (32.9％) of them got clinical pregnancy,the implantation rate was 15.2％(90/594).The embryo survival rate,intact survival rate,pregnant rate,implantation rate were higher in vitrification group than those in slow-freezing group (P ＜ 0.05).Conclusion Vitrification is a more effective method of cryopreserving the human embryo than conventional slow freezing.
