CAJ | 학술논문

目的探讨适用于放疗增敏研究中人卵巢癌SKOV3乏氧细胞模型的建立方法.方法将人卵巢癌细胞株SKOV3分为常氧对照组、二氯化钴组和环境乏氧组,各组均在X射线单次照射(0、2、4、6、8 Gy)后72 h,应用噻唑蓝法检测细胞增殖.结果与常氧对照组未照射细胞相比,二氯化钴组和环境乏氧组未照射细胞的细胞增殖率均显著降低(t=24.789、196.960,P均＜0.01);与同组未照射细胞相比,常氧对照组与二氯化钴组接受不同剂量的X射线单次照射后,其细胞存活率均显著性下降(F=2263.039、3672.044,P均＜0.01),且放射剂量越大,其细胞存活率越低,而环境乏氧组无显著性变化(F=1.412,P＞0.05);与常氧对照组、二氯化钴组的同剂量照射细胞相比,环境乏氧组细胞存活率均显著升高(2Gy:F=61.125; 4Gy:F=181.825;6Gy:F=373.830; 8Gy:F=2425.510,P均＜0.01).结论环境乏氧组的细胞对射线产生了强烈的抵抗性,即放射敏感性显著性降低,射线对其杀伤力减弱,且所获得的乏氧细胞模型明显优于二氯化钴组,因此环境乏氧比二氯化钴更适合作为放疗增敏研究中人卵巢癌SKOV3乏氧细胞模型的建立方法.
목적 탐토괄용우방료증민연구중인란소암SKOV3핍양세포모형적건립방법.방법 장인란소암세포주SKOV3분위상양대조조、이록화고조화배경핍양조,각조균재X사선단차조사(0、2、4、6、8 Gy)후72 h,응용새서람법검측세포증식.결과 여상양대조조미조사세포상비,이록화고조화배경핍양조미조사세포적세포증식솔균현저강저(t=24.789、196.960,P균＜0.01);여동조미조사세포상비,상양대조조여이록화고조접수불동제량적X사선단차조사후,기세포존활솔균현저성하강(F=2263.039、3672.044,P균＜0.01),차방사제량월대,기세포존활솔월저,이배경핍양조무현저성변화(F=1.412,P＞0.05);여상양대조조、이록화고조적동제량조사세포상비,배경핍양조세포존활솔균현저승고(2Gy:F=61.125; 4Gy:F=181.825;6Gy:F=373.830; 8Gy:F=2425.510,P균＜0.01).결론 배경핍양조적세포대사선산생료강렬적저항성,즉방사민감성현저성강저,사선대기살상력감약,차소획득적핍양세포모형명현우우이록화고조,인차배경핍양비이록화고경괄합작위방료증민연구중인란소암SKOV3핍양세포모형적건립방법.
Objective To investigate the suitable method of establishing the hypoxic SKOV3 cellular model in the research of radiotherapy sensitizer.Methods Human ovarian cancer cell line SKOV3 were divided into three groups:normoxic control group,cobalt chloride group and environmental hypoxia group.Cell proliferation of each group 72 h after single X-ray irradiation(0,2,4,6,8 Gy)were detected by MTT assay.Results Cell proliferation of non-irradiated cells in cobalt chloride group and environmental hypoxia group were significantly lower than that in normoxic control group (t =24.789,196.960,both P＜0.01).Cell viability of the cells in normoxic control group and cobalt chloride group were significantly decreased after receiving a single X-ray irradiation of different doses compared with non-irradiated cells in the same group(F=2263.039,3672.044,bothP＜0.01) and their cell viability were decreased with the increase of radiation dose,while environmental hypoxia group had no significant change (F=1.412,P＞0.05).The cell viability of irradiated cells in environmental hypoxia group were significantly higher than the irradiated cells with the same radiation in normoxic control group and cobalt chloride group(2 Gy:F=61.125; 4 Gy:F=181.825; 6 Gy:F=373.830; 8 Gy:F=2425.510,all P＜0.01).Conclusions Cells in environmental hypoxia group were very resistant to radiation.Their radiation sensitivity strikingly decreased and the killing effect of radiation on them was weak.The hypoxic cellular model of environmental hypoxia group was obviously superior to cobalt chloride group.Environmental hypoxia method was more suitable to establish the hypoxic SKOV3 cellular model in the research of radiotherapy sensitizer compared with cobalt chloride.