CAJ | 학술논문

目的探讨蛋白磷酸酶1调节亚基11(PPP1R11)基因多态性与丙型肝炎病毒(HCV)感染自发清除的相关性.方法选择2009年1月至2013年5月于广州血液中心行无偿献血的献血者中,血液样品HCV抗体的酶联免疫吸附试验(ELISA)检测结果呈阳性的328例献血者为研究对象.将研究对象随访结果中,HCV RNA检测结果呈阴性,但经重组免疫印迹试验(RIBA)确证为HCV抗体呈阳性献血者纳入自发清除组(n=134);HCV RNA检测结果呈阳性献血者纳入慢性感染组(n=194).采用序列特异性引物聚合酶链反应(PCR-SSP)检测PPP1 R11基因rs3807031位点基因型,分析其与HCV自发清除的相关性.结果自发清除组献血者的平均年龄为(28.8±10.2)岁低于慢性感染组的(31.0±9.0)岁.二者比较,差异有统计学意义(t=2.135,P=0.034).自发清除组中女性比例为35.8％,高于慢性感染组的21.6％.二者比较,差异有统计学意义(x2 =7.994,P=0.005). rs3807031的等位基因A和C在自发清除组的基因频率分别为30.6％和69.4％,在慢性感染组中则为22.4％和77.6％.两组献血者rs3807031等位基因频率比较,差异有统计学意义(x2 =5.538,P=0.023).多因素非条件logistic回归分析结果表明,性别(OR=1.957,95％CI:1.118～3.224,P=0.008)和rs3807031基因型(OR=0.650,95％CI:0.450～0.973,P=0.021)是HCV自发清除的独立影响因素.结论 PPP1R11基因rs3807031位点基因型与HCV自发清除显著相关,可作为HCV感染自发清除的预测因素,为进一步研究机体自发清除HCV的机制及分子标志物提供了科学证据.
목적 탐토단백린산매1조절아기11(PPP1R11)기인다태성여병형간염병독(HCV)감염자발청제적상관성.방법 선택2009년1월지2013년5월우엄주혈액중심행무상헌혈적헌혈자중,혈액양품HCV항체적매련면역흡부시험(ELISA)검측결과정양성적328례헌혈자위연구대상.장연구대상수방결과중,HCV RNA검측결과정음성,단경중조면역인적시험(RIBA)학증위HCV항체정양성헌혈자납입자발청제조(n=134);HCV RNA검측결과정양성헌혈자납입만성감염조(n=194).채용서렬특이성인물취합매련반응(PCR-SSP)검측PPP1 R11기인rs3807031위점기인형,분석기여HCV자발청제적상관성.결과 자발청제조헌혈자적평균년령위(28.8±10.2)세저우만성감염조적(31.0±9.0)세.이자비교,차이유통계학의의(t=2.135,P=0.034).자발청제조중녀성비례위35.8％,고우만성감염조적21.6％.이자비교,차이유통계학의의(x2 =7.994,P=0.005). rs3807031적등위기인A화C재자발청제조적기인빈솔분별위30.6％화69.4％,재만성감염조중칙위22.4％화77.6％.량조헌혈자rs3807031등위기인빈솔비교,차이유통계학의의(x2 =5.538,P=0.023).다인소비조건logistic회귀분석결과표명,성별(OR=1.957,95％CI:1.118～3.224,P=0.008)화rs3807031기인형(OR=0.650,95％CI:0.450～0.973,P=0.021)시HCV자발청제적독립영향인소.결론 PPP1R11기인rs3807031위점기인형여HCV자발청제현저상관,가작위HCV감염자발청제적예측인소,위진일보연구궤체자발청제HCV적궤제급분자표지물제공료과학증거.
Objective To explore the association of protein phosphatase-1 regulatory inhibitor subunit-11 (PPP1R11) polymorphism and hepatitis C virus (HCV) spontaneous clearance.Methods From January 2009 to May 2013,a total of 328 blood donors whose blood samples were anti-HCV positive with enzyme-linked immunosorbent assay (ELISA) were included into this study.According to follow-up results,they were divided into spontaneous clearance group whose HCV RNA were negative but anti-HCV recombinant immunoblot assay results were positive (n =134),and chronic infection group whose HCV RNA were positive (n=198).Polymerase chain reaction with sequence specific primer(PCR-SSP) was performed to determine the genotype of rs3807031 of PPP1R11.Statistical software SPSS was used to analyze the association between genotype of rs3807031 and HCV spontaneous clearance.Results The average age of donors in spontaneous clearance group was (28.8± 10.2) years old,which was significantly lower than that of chronic infection group whose average age was (31.0±9.0) years old (t=2.135,P =0.034).Higher percentage of female was observed in spontaneous clearance group compared to chronic infection group (35.8％ vs 21.6％ ; x2 =7.994,P=0.005).Importantly,rs3807031 A allele composed 30.6％ and 22.4％,while C allele composed 69.4％ and 77.6％ in spontaneous clearance and chronic infection group,respectively.The frequencies of rs3807031 alleles were statistically different between these two groups (x2 =5.538,P =0.023).Multivariate unconditional logistic regression analysis showed that gender (OR=1.957,95％CI:1.118-3.224,P=0.008) and genotype of rs3807031 (OR=0.650,95％CI:0.450-0.973,P =0.021) are independent factors associated with HCV spontaneous clearance.Conclusions PPP1R11 rs3807031 is associated with HCV spontaneous clearance,which has the potential to predict the spontaneous clearance of HCV infection.Our results provide information to the research on the mechanism and molecular marker of HCV spontaneous clearance.