中华急诊医学杂志
中華急診醫學雜誌
중화급진의학잡지
CHINESE JOURNAL OF EMERGENCY MEDICINE
2014年
12期
1344-1347
,共4页
陆如凤%黄小民%吴海波%张卓一%丁黎敏%吴丽娟%徐俪颖
陸如鳳%黃小民%吳海波%張卓一%丁黎敏%吳麗娟%徐儷穎
륙여봉%황소민%오해파%장탁일%정려민%오려연%서려영
百草枯%急性肺损伤%大鼠%肺组织%Toll样受体4%核因子-κB%肿瘤坏死因子-α%白细胞介素-6
百草枯%急性肺損傷%大鼠%肺組織%Toll樣受體4%覈因子-κB%腫瘤壞死因子-α%白細胞介素-6
백초고%급성폐손상%대서%폐조직%Toll양수체4%핵인자-κB%종류배사인자-α%백세포개소-6
Paraquat%Acute lung injury%Rat%Lung tissue%Toll-like receptor 4%Nuclear factor-κB%Tumor necrosis factor-α%Interleukin-6
目的 观察急性百草枯(PQ)中毒大鼠肺组织中Toll样受体4(TLR4)和核因子-κB (NF-κB) mRNA表达的变化,探讨TLR4-NF-κB通路在急性PQ中毒肺损伤中的作用.方法 30只雄性SD大鼠随机分为2组:生理盐水(NS)对照组(6只,NS腹腔注射),PQ染毒组(24只,腹腔注射PQ溶液,20 mg/kg).PQ染毒组分别在染毒后6、24、48 h及72 h麻醉处死,NS对照组于处理后6h处死,进行肺组织HE染色,病理学观察,用实时定量PCR方法检测TLR4和NF-κB的mRNA表达情况,用ELISA方法测定肺组织中肿瘤坏死因子-α(TNF-α)、血清中IL-6含量.结果 PQ染毒组大鼠肺组织病理表现为早期充血水肿明显,大量炎性细胞浸润.与NS对照组相比PQ染毒组肺组织中TLR4和NF-κB mRNA的表达量、TNF-α、IL-6含量在各时间点均有不同程度升高,差异均具有统计学意义(P＜0.01).结论 大鼠急性PQ中毒后肺组织中TLR4和NF-κBmRNA表达量、TNF-α、IL-6含量明显上调,TLR4-NF-κB通路参与了PQ中毒大鼠肺损伤的早期病理生理过程.
目的 觀察急性百草枯(PQ)中毒大鼠肺組織中Toll樣受體4(TLR4)和覈因子-κB (NF-κB) mRNA錶達的變化,探討TLR4-NF-κB通路在急性PQ中毒肺損傷中的作用.方法 30隻雄性SD大鼠隨機分為2組:生理鹽水(NS)對照組(6隻,NS腹腔註射),PQ染毒組(24隻,腹腔註射PQ溶液,20 mg/kg).PQ染毒組分彆在染毒後6、24、48 h及72 h痳醉處死,NS對照組于處理後6h處死,進行肺組織HE染色,病理學觀察,用實時定量PCR方法檢測TLR4和NF-κB的mRNA錶達情況,用ELISA方法測定肺組織中腫瘤壞死因子-α(TNF-α)、血清中IL-6含量.結果 PQ染毒組大鼠肺組織病理錶現為早期充血水腫明顯,大量炎性細胞浸潤.與NS對照組相比PQ染毒組肺組織中TLR4和NF-κB mRNA的錶達量、TNF-α、IL-6含量在各時間點均有不同程度升高,差異均具有統計學意義(P＜0.01).結論 大鼠急性PQ中毒後肺組織中TLR4和NF-κBmRNA錶達量、TNF-α、IL-6含量明顯上調,TLR4-NF-κB通路參與瞭PQ中毒大鼠肺損傷的早期病理生理過程.
목적 관찰급성백초고(PQ)중독대서폐조직중Toll양수체4(TLR4)화핵인자-κB (NF-κB) mRNA표체적변화,탐토TLR4-NF-κB통로재급성PQ중독폐손상중적작용.방법 30지웅성SD대서수궤분위2조:생리염수(NS)대조조(6지,NS복강주사),PQ염독조(24지,복강주사PQ용액,20 mg/kg).PQ염독조분별재염독후6、24、48 h급72 h마취처사,NS대조조우처리후6h처사,진행폐조직HE염색,병이학관찰,용실시정량PCR방법검측TLR4화NF-κB적mRNA표체정황,용ELISA방법측정폐조직중종류배사인자-α(TNF-α)、혈청중IL-6함량.결과 PQ염독조대서폐조직병리표현위조기충혈수종명현,대량염성세포침윤.여NS대조조상비PQ염독조폐조직중TLR4화NF-κB mRNA적표체량、TNF-α、IL-6함량재각시간점균유불동정도승고,차이균구유통계학의의(P＜0.01).결론 대서급성PQ중독후폐조직중TLR4화NF-κBmRNA표체량、TNF-α、IL-6함량명현상조,TLR4-NF-κB통로삼여료PQ중독대서폐손상적조기병리생리과정.
Objective To investigate the expressions of toll-like receptor 4 mRNA and nuclear factor-κB (TLR4-NF-κB) mRNA in lung tissue of rats with acute paraquat poisoning.Methods Thirty male SD rats were randomly divided into two groups,namely normal saline group (NS group,n =6) and the PQ groups (n =24).The rats of PQ group were administered with 20 mg/kg PQ by intraperitoneal injection to establish the models of PQ induced lung injury.The saline was administered once instead in normal saline group.The rats of PQ group were sacrificed separately at 6 h,12 h,24 h,48 h and 72 hours after modeling,while at 6 hours the rats of normal saline group was sacrificed.The expressions of TLR4 mRNA,NF-κB mRNA,and the levels of tumor necrosis factor-α and interleukin-6 in lung (serum) of rats were detected.Meanwhile,pathological changes of the lung tissue were examined under optical microscope.Results There were no histopathological changes such as alveolar edema,hemorrhage and inflammatory cell infiltration found in NS group other than in PQ group.At the 72 h,the expressions of TLR4 mRNA (9.61 ±0.21),NF-κB mRNA (5.62 ± 0.24),and the levels of TNF-α (153.08 ± 5.02) pg/ml,and interleukin-6 (230.08 ± 10.64) pg/mL in lung tissue of rats were significantly higher in PQ group than those in control group (P ＜ 0.01).Conclusions In rats after PQ poisoning,the expressions of TLR4 mRNA,NF-κB mRNA,and the levels of TNF-αand IL-6 were higher than those in normal rats,and the TLR4-NF-κB pathway could play an important role in lung injury of rats with PQ poisoning.
