中华实验眼科杂志
中華實驗眼科雜誌
중화실험안과잡지
CHINESE JOURNAL OF EXPERIMENTAL OPHTHALMOLOGY
2014年
12期
1080-1083
,共4页
可重复性%眼压计%高眼压,遗传性
可重複性%眼壓計%高眼壓,遺傳性
가중복성%안압계%고안압,유전성
Repeatability and reproducibility%Tonometer%Ocular hypertension,genetic
背景 准确的眼压测量是青光眼实验研究的基础.TonoLab是一种新型的动物眼压计,其测量眼压的可重复性尚有待验证. 目的 评估新型眼压计TonoLab在遗传性高眼压模型中的准确性. 方法 采用新型眼压计TonoLab对麻醉状态下的63只7月龄遗传性高眼压DBA/2J小鼠进行眼压测量,测量时由两位技术娴熟的操作者分别、连续测量同一只眼的眼压3次,测量不分先后顺序,采用操作者盲法获取测量数据.计算测量结果的组内标准差(Sw)、组内相关系数(ICC)及组内变异系数(CVw),以评估每位操作者测量结果的可重复性,采用Bland-Altman一致性检验评估两位操作者之间测量结果的可重复性. 结果 共测量63只小鼠126只眼,其中正常眼压者105只眼,操作者1测得的平均眼压为(12.98±1.05) mmHg(1 mmHg=0.133 kPa),操作者2为(12.41±0.92) mmHg;高眼压者21只眼,操作者1和操作者2测得的平均眼压分别为(27.76±1.95) mmHg和(27.49±1.28) mmHg.正常眼压组两位操作者测量结果的Sw、ICC及CVw分别为1.34/1.11 mmHg、0.90/0.92和8.24％/7.42％;高眼压组两位操作者测量结果的Sw、ICC及CVw分别为2.21/1.49 mmHg、0.89/0.95和6.97％/4.82％.正常眼压组两位操作者测量结果之间的平均偏差为0.60 mmHg[95％可信区间(CI):-5.20 ～6.30 mmHg],高眼压组为0.30 mmHg(95％ CI:-7.30～ 7.80 mmHg).结论 TonoLab眼压计在正常眼压及高眼压小鼠中单个操作者测量结果的组内可重复性高,但两位操作者测量结果之间的可重复性较低,因此单个操作者用TonoLab眼压计测得的眼压结果可靠、准确.
揹景 準確的眼壓測量是青光眼實驗研究的基礎.TonoLab是一種新型的動物眼壓計,其測量眼壓的可重複性尚有待驗證. 目的 評估新型眼壓計TonoLab在遺傳性高眼壓模型中的準確性. 方法 採用新型眼壓計TonoLab對痳醉狀態下的63隻7月齡遺傳性高眼壓DBA/2J小鼠進行眼壓測量,測量時由兩位技術嫻熟的操作者分彆、連續測量同一隻眼的眼壓3次,測量不分先後順序,採用操作者盲法穫取測量數據.計算測量結果的組內標準差(Sw)、組內相關繫數(ICC)及組內變異繫數(CVw),以評估每位操作者測量結果的可重複性,採用Bland-Altman一緻性檢驗評估兩位操作者之間測量結果的可重複性. 結果 共測量63隻小鼠126隻眼,其中正常眼壓者105隻眼,操作者1測得的平均眼壓為(12.98±1.05) mmHg(1 mmHg=0.133 kPa),操作者2為(12.41±0.92) mmHg;高眼壓者21隻眼,操作者1和操作者2測得的平均眼壓分彆為(27.76±1.95) mmHg和(27.49±1.28) mmHg.正常眼壓組兩位操作者測量結果的Sw、ICC及CVw分彆為1.34/1.11 mmHg、0.90/0.92和8.24％/7.42％;高眼壓組兩位操作者測量結果的Sw、ICC及CVw分彆為2.21/1.49 mmHg、0.89/0.95和6.97％/4.82％.正常眼壓組兩位操作者測量結果之間的平均偏差為0.60 mmHg[95％可信區間(CI):-5.20 ～6.30 mmHg],高眼壓組為0.30 mmHg(95％ CI:-7.30～ 7.80 mmHg).結論 TonoLab眼壓計在正常眼壓及高眼壓小鼠中單箇操作者測量結果的組內可重複性高,但兩位操作者測量結果之間的可重複性較低,因此單箇操作者用TonoLab眼壓計測得的眼壓結果可靠、準確.
배경 준학적안압측량시청광안실험연구적기출.TonoLab시일충신형적동물안압계,기측량안압적가중복성상유대험증. 목적 평고신형안압계TonoLab재유전성고안압모형중적준학성. 방법 채용신형안압계TonoLab대마취상태하적63지7월령유전성고안압DBA/2J소서진행안압측량,측량시유량위기술한숙적조작자분별、련속측량동일지안적안압3차,측량불분선후순서,채용조작자맹법획취측량수거.계산측량결과적조내표준차(Sw)、조내상관계수(ICC)급조내변이계수(CVw),이평고매위조작자측량결과적가중복성,채용Bland-Altman일치성검험평고량위조작자지간측량결과적가중복성. 결과 공측량63지소서126지안,기중정상안압자105지안,조작자1측득적평균안압위(12.98±1.05) mmHg(1 mmHg=0.133 kPa),조작자2위(12.41±0.92) mmHg;고안압자21지안,조작자1화조작자2측득적평균안압분별위(27.76±1.95) mmHg화(27.49±1.28) mmHg.정상안압조량위조작자측량결과적Sw、ICC급CVw분별위1.34/1.11 mmHg、0.90/0.92화8.24％/7.42％;고안압조량위조작자측량결과적Sw、ICC급CVw분별위2.21/1.49 mmHg、0.89/0.95화6.97％/4.82％.정상안압조량위조작자측량결과지간적평균편차위0.60 mmHg[95％가신구간(CI):-5.20 ～6.30 mmHg],고안압조위0.30 mmHg(95％ CI:-7.30～ 7.80 mmHg).결론 TonoLab안압계재정상안압급고안압소서중단개조작자측량결과적조내가중복성고,단량위조작자측량결과지간적가중복성교저,인차단개조작자용TonoLab안압계측득적안압결과가고、준학.
Background Reliable intraocular pressure (IOP) measurement is essential in glaucomatous study.TonoLab is a newly designed tonometer for small animals,however,its repeatability and reproducibility remains unknown.Objective This study aimed to evaluate the repeatability and reproducibility of TonoLab rebound tonometer in DBA/2J mice.Methods Intraocular pressure was measured in both eyes of 63 DBA/2J mice aged 7 months under systemic anesthesia with TonoLab rebound tonometer.IOP of each eye was measured for 3 times by two observers independently and consecutively using TonoLab rebound tonometer,and the data were obtained in single blind method.Within standard deviation (Sw),interclass correlation coefficient (ICC),within coefficient of variation (CVw) and corresponding 95％ confidence interval (CI) were calculated to evaluate the repeatability of measuring result from single observer,and Bland-Altman method was used to assess the agreement between two observers.The abnormal data which affected repeatability was analyzed.Results Among the 126 eyes,105 eyes showed the normal IOP with mean value (12.98 ± 1.05) mmHg from observer 1 and (12.41 ±0.92) mmHg from observer 2.Ocular hypertension was found in 21 eyes with mean value (27.76 ± 1.95) mmHg and (27.49 ± 1.28) mmHg for observer 1 and observer 2 respectively.Sw,ICC and CVw for observer 1/observer 2 were 1.34/1.11 mmHg,0.90/0.92,8.24％/7.42％ in normal eyes and 2.21/1.49 mmHg,0.89/0.95,6.97％/4.82％ in hypertensive eyes.The average bias between two measuring values from different observers was 0.60 mmHg in the normal eyes (95％ CI:-5.20-6.30 mmHg) and 0.30 mmHg in the hypertensive eyes (95％ CI:-7.30-7.80 mmHg).Conclusions TonoLab rebound tonometer can offer a good intraobserver repeatability in both normal and hypertensive eyes in mice,but the agreement between two observers is outside an acceptable level.So we consider IOP in mice obtained by TonoLab rebound tonometer is reliable and accurate from a single observer.
