中华实验眼科杂志
中華實驗眼科雜誌
중화실험안과잡지
CHINESE JOURNAL OF EXPERIMENTAL OPHTHALMOLOGY
2014年
12期
1084-1087
,共4页
赵敏%白玉婧%王斌%梁建宏%赵明威%黎晓新%石璇
趙敏%白玉婧%王斌%樑建宏%趙明威%黎曉新%石璇
조민%백옥청%왕빈%량건굉%조명위%려효신%석선
血管内皮生长因子/VEGFxxx家族,VEGFxxxb家族%年龄相关性黄斑变性/新生血管性%脉络膜新生血管%脉络膜息肉样病变
血管內皮生長因子/VEGFxxx傢族,VEGFxxxb傢族%年齡相關性黃斑變性/新生血管性%脈絡膜新生血管%脈絡膜息肉樣病變
혈관내피생장인자/VEGFxxx가족,VEGFxxxb가족%년령상관성황반변성/신생혈관성%맥락막신생혈관%맥락막식육양병변
Vascular endothelial growth factor/VEGFxxx family,VEGFxxx b family%Age-related macular degeneration/neovascular%Choroidal neovascularization%Polypoidal choroidal vasculopathy
背景 息肉状脉络膜血管病变(PCV)是湿性年龄相关性黄斑变性(nAMD)的一个亚型还是独立于nAMD一种病变目前仍有不同的观点,而两种疾病的治疗效果可能不同.抗血管内皮生长因子(VEGF)在nAMD的治疗中发挥重要作用,但由VEGF可变剪切而产生的促新生血管生成VEGF(VEGFxxx)家族和抑新生血管VEGF(VEGFxxxb)家族,两者功能相反,其在nAMD和PCV中的表达及作用尚不清楚. 目的 检测和比较VEGFxxx家族和VEGFxxxb家族在nAMD患者和PCV患者房水中的变化及其意义. 方法 收集2013年3-12月在北京大学人民医院眼科确诊的nAMD患者34例及PCV患者26例,同期纳入16例年龄相关性白内障患者作为对照.收集被检者前房水0.1 ml,采用酶联免疫吸附试验(ELISA)测定前房水中VEGF及VEGF165b质量浓度,比较3组患者房水中VEGF及VEGF165b质量浓度的差异. 结果 nAMD组、PCV组、对照组患者房水中VEGF质量浓度分别为(4 210.00±998.40)、(387.00±51.31)和(377.40±69.97) pg/ml,组间总体差异有统计学意义(F=12.851,P=0.000).nAMD组、PCV组、对照组VEGF165b质量浓度分别为(205.50±12.59)、(159.40± 16.25)、(347.90±29.18) pg/ml,组间总体比较差异有统计学意义(F=23.752,P=0.000).与对照组比较,nAMD组患眼房水中VEGF质量浓度升高,VEGF165b质量浓度下降,PCV组患眼房水中VEGF165b表达下降,差异均有统计学意义(均P=0.000),但PCV组与对照组患眼房水中VEGF含量差异无统计学意义(P=0.992).nAMD组较PCV组房水中VEGF质量浓度升高,差异有统计学意义(P=0.001),但两组间VEGF165b质量浓度差异无统计学意义(P=0.097). 结论 nAMD患者前房水中的VEGF质量浓度明显高于PCV患者和年龄相关性白内障患者,nAMD患者与PCV患者房水中VEGF165b质量浓度接近,但均低于年龄相关性白内障患者,说明VEGF165b质量浓度的相对下降与nAMD和PCV的发生和发展密切相关.
揹景 息肉狀脈絡膜血管病變(PCV)是濕性年齡相關性黃斑變性(nAMD)的一箇亞型還是獨立于nAMD一種病變目前仍有不同的觀點,而兩種疾病的治療效果可能不同.抗血管內皮生長因子(VEGF)在nAMD的治療中髮揮重要作用,但由VEGF可變剪切而產生的促新生血管生成VEGF(VEGFxxx)傢族和抑新生血管VEGF(VEGFxxxb)傢族,兩者功能相反,其在nAMD和PCV中的錶達及作用尚不清楚. 目的 檢測和比較VEGFxxx傢族和VEGFxxxb傢族在nAMD患者和PCV患者房水中的變化及其意義. 方法 收集2013年3-12月在北京大學人民醫院眼科確診的nAMD患者34例及PCV患者26例,同期納入16例年齡相關性白內障患者作為對照.收集被檢者前房水0.1 ml,採用酶聯免疫吸附試驗(ELISA)測定前房水中VEGF及VEGF165b質量濃度,比較3組患者房水中VEGF及VEGF165b質量濃度的差異. 結果 nAMD組、PCV組、對照組患者房水中VEGF質量濃度分彆為(4 210.00±998.40)、(387.00±51.31)和(377.40±69.97) pg/ml,組間總體差異有統計學意義(F=12.851,P=0.000).nAMD組、PCV組、對照組VEGF165b質量濃度分彆為(205.50±12.59)、(159.40± 16.25)、(347.90±29.18) pg/ml,組間總體比較差異有統計學意義(F=23.752,P=0.000).與對照組比較,nAMD組患眼房水中VEGF質量濃度升高,VEGF165b質量濃度下降,PCV組患眼房水中VEGF165b錶達下降,差異均有統計學意義(均P=0.000),但PCV組與對照組患眼房水中VEGF含量差異無統計學意義(P=0.992).nAMD組較PCV組房水中VEGF質量濃度升高,差異有統計學意義(P=0.001),但兩組間VEGF165b質量濃度差異無統計學意義(P=0.097). 結論 nAMD患者前房水中的VEGF質量濃度明顯高于PCV患者和年齡相關性白內障患者,nAMD患者與PCV患者房水中VEGF165b質量濃度接近,但均低于年齡相關性白內障患者,說明VEGF165b質量濃度的相對下降與nAMD和PCV的髮生和髮展密切相關.
배경 식육상맥락막혈관병변(PCV)시습성년령상관성황반변성(nAMD)적일개아형환시독립우nAMD일충병변목전잉유불동적관점,이량충질병적치료효과가능불동.항혈관내피생장인자(VEGF)재nAMD적치료중발휘중요작용,단유VEGF가변전절이산생적촉신생혈관생성VEGF(VEGFxxx)가족화억신생혈관VEGF(VEGFxxxb)가족,량자공능상반,기재nAMD화PCV중적표체급작용상불청초. 목적 검측화비교VEGFxxx가족화VEGFxxxb가족재nAMD환자화PCV환자방수중적변화급기의의. 방법 수집2013년3-12월재북경대학인민의원안과학진적nAMD환자34례급PCV환자26례,동기납입16례년령상관성백내장환자작위대조.수집피검자전방수0.1 ml,채용매련면역흡부시험(ELISA)측정전방수중VEGF급VEGF165b질량농도,비교3조환자방수중VEGF급VEGF165b질량농도적차이. 결과 nAMD조、PCV조、대조조환자방수중VEGF질량농도분별위(4 210.00±998.40)、(387.00±51.31)화(377.40±69.97) pg/ml,조간총체차이유통계학의의(F=12.851,P=0.000).nAMD조、PCV조、대조조VEGF165b질량농도분별위(205.50±12.59)、(159.40± 16.25)、(347.90±29.18) pg/ml,조간총체비교차이유통계학의의(F=23.752,P=0.000).여대조조비교,nAMD조환안방수중VEGF질량농도승고,VEGF165b질량농도하강,PCV조환안방수중VEGF165b표체하강,차이균유통계학의의(균P=0.000),단PCV조여대조조환안방수중VEGF함량차이무통계학의의(P=0.992).nAMD조교PCV조방수중VEGF질량농도승고,차이유통계학의의(P=0.001),단량조간VEGF165b질량농도차이무통계학의의(P=0.097). 결론 nAMD환자전방수중적VEGF질량농도명현고우PCV환자화년령상관성백내장환자,nAMD환자여PCV환자방수중VEGF165b질량농도접근,단균저우년령상관성백내장환자,설명VEGF165b질량농도적상대하강여nAMD화PCV적발생화발전밀절상관.
Background There remains some controversy over whether polypoidal choroidal vasculopathy (PCV) represents a subtype of neovascular age-related macular degeneration (nAMD).Anti-vascular endothelial growth factor (VEGF) therapies are important in the treatment of PCV and nAMD.It has been identified that VEGF-A was differentially spliced from exons 8 and formed two isoforms families:the pro-angiogenic VEGFxxx family and the anti-angiogenic VEGFxxxb family.However,the role of the two VEGF families in PCV and nAMD was still unclear.Objective This study was to measure the contents of pro-angiogenic VEGFxxx family and the anti-angiogenic VEGFxxxb family in aqueous humor of nAMD and PCV patients and explore their effect on nAMD and PCV.Methods Thirty-four nAMD patients and 26 PCV patients were enrolled in Peking University People's Hospital during March to December,2013,and 16 age-related cataract patients served as controls.The aqueous humor samples 0.1 ml was collected before the introvitreous injection of anti-VEGF drug.The contents of pro-angiogenic VEGFxxx family and the anti-angiogenic VEGFxxx b family in the aqueous humor were measured by enzyme-linked immunosorbent assay(ELISA).Results The concentrations of VEGF in the aqueous humor in the nAMD group,PCV group and control group were (4 210.00±998.40),(387.00±51.31) and (377.40 ±69.97)pg/ml,respectively,showing a significant difference among the three groups (F =12.851,P =0.000).The concentrations of VEGF165 b in the aqueous humor in the nAMD group,PCV group and control group were (205.50±12.59),(159.40±16.25) and (347.90±29.18) pg/ml,with a significant difference among them (F=23.752,P=0.000).Compared with the control group,VEGF content in the aqueous humor was elevated and the VEGF165b content was declined in the nAMD group,and VEGF165b was lowed in the PCV group,with significant differences between them(all at P=0.000).However,no significant difference was seen in the change of VEGF between the PCV group and the control group (P=0.992).The VEGF content in the aqueous humor was higher in the nAMD group than that in the PCV group (P =0.001),but VEGF165b content was insignificantly different (P =0.097).Conclusions The downregulation of VEGFxxx b may be associated with nAMD and PCV.The different role of VEGFxxx b in the development of PCV and nAMD needs to be verified in further studies.
