CAJ | 학술논문

目的制备雷帕霉素(RAPA)脂质体滴眼液并研究RAPA对碱烧伤后大鼠角膜新生血管(corneal neovascularization,CNV)的抑制作用.方法利用薄膜分散法制备RAPA脂质体滴眼液.采用成组设计法,将102只健康Wistar大鼠分组,碱烧伤后每日裂隙灯显微镜观察眼局部情况,并详细记录碱烧伤后大鼠角膜反应情况与新生血管生长情况,分别于碱烧伤后1d、4d、7d、14d随机抽取各组大鼠取材,行HE染色分析.实验结果采用方差分析及组间q检验进行分析.结果 (1)制得的RAPA脂质体,为完整的类球形,平均粒径为145.2 nm,包封率为90.02％,RAPA脂质体滴眼液中RAPA含量高,粒径合适,可作为滴眼液用于动物实验.(2) RAPA脂质体滴眼液组CNV生长迟缓、稀疏,退化较早;CNV面积较小,差异有统计学意义(P＜0.05).结论脂质体是RAPA良好的药物载体.RAPA能显著抑制CNV的生长,有望成为防治CNV的一种有效方法.
목적 제비뢰파매소(RAPA)지질체적안액병연구RAPA대감소상후대서각막신생혈관(corneal neovascularization,CNV)적억제작용.방법 이용박막분산법제비RAPA지질체적안액.채용성조설계법,장102지건강Wistar대서분조,감소상후매일렬극등현미경관찰안국부정황,병상세기록감소상후대서각막반응정황여신생혈관생장정황,분별우감소상후1d、4d、7d、14d수궤추취각조대서취재,행HE염색분석.실험결과채용방차분석급조간q검험진행분석.결과 (1)제득적RAPA지질체,위완정적류구형,평균립경위145.2 nm,포봉솔위90.02％,RAPA지질체적안액중RAPA함량고,립경합괄,가작위적안액용우동물실험.(2) RAPA지질체적안액조CNV생장지완、희소,퇴화교조;CNV면적교소,차이유통계학의의(P＜0.05).결론 지질체시RAPA량호적약물재체.RAPA능현저억제CNV적생장,유망성위방치CNV적일충유효방법.
Objective To prepare rapamycin liposomes gutta,and to evaluate their effect of inhibiting corneal neovascularization of rats induced by alkali cauterization.Methods Rapamycin liposomes were prepared by thin film hydration.Of 102 Wistar rats were divided into four groups at random.All right eyes of 96 rats were induced by alkali cauterization.After alkali burn,cauterized rats were observed by slitlamp biomicroscope every day.The analysis of variance and q test groups for analysis were adopted to analyze the results.Results The body of RAPA liposome was intact kinds of spheres,the average diameter was 145.2nm,and the envelopment rate was 90.02％.RAPA liposome could be used for animal's experiment as drip eyes liquid.The CNV of the rapamycin liposome treated group slowly and smaller than other group (P ＜0.05).Conclusions Liposome body is an excellent medicine carrier for the RAPA.RAPA can obviously suppress the growth of CNV.