CAJ | 학술논문

目的为探讨低剂量电离辐射生物效应作用机制,研究不同剂量单次照射后,人淋巴母细胞蛋白表达的变化.方法利用双向凝胶电泳和基质辅助激光解析飞行时间串联质谱技术分离、鉴定不同低剂量γ射线导致的人淋巴母细胞差异表达蛋白,采用实时荧光定量PCR法从mRNA水平验证蛋白的差异表达.结果不同低剂量γ射线照射后,双向电泳和质谱分离鉴定得到3个差异表达点:真核翻译起始因子5A(eIF5A),前折叠素亚单位1(PFDN1),脂肪酸结合蛋白4(FA BP4).其中,eIF5A和FABP4的mRNA水平表达变化与双向电泳结果一致.结论低剂量γ射线导致人淋巴母细胞中eIF5A、PFDN1和FABP4等蛋白表达发生变化,差异表达的蛋白有助于阐明低剂量辐射生物效应作用机制,并为进一步研究低剂量辐射暴露的生物标志物提供了参考.
목적 위탐토저제량전리복사생물효응작용궤제,연구불동제량단차조사후,인림파모세포단백표체적변화.방법 이용쌍향응효전영화기질보조격광해석비행시간천련질보기술분리、감정불동저제량γ사선도치적인림파모세포차이표체단백,채용실시형광정량PCR법종mRNA수평험증단백적차이표체.결과 불동저제량γ사선조사후,쌍향전영화질보분리감정득도3개차이표체점:진핵번역기시인자5A(eIF5A),전절첩소아단위1(PFDN1),지방산결합단백4(FA BP4).기중,eIF5A화FABP4적mRNA수평표체변화여쌍향전영결과일치.결론 저제량γ사선도치인림파모세포중eIF5A、PFDN1화FABP4등단백표체발생변화,차이표체적단백유조우천명저제량복사생물효응작용궤제,병위진일보연구저제량복사폭로적생물표지물제공료삼고.
Objective To observe the alterations of protein expression in human normal lymphoblastoid cell AHH-1 lines,after irradiation with different doses of γ-rays,in order to investigate mechanisms of biological effects induced by low dose radiation.Methods Two-dimensional electrophoresis (2-DE) was performed and differential expression proteins were identified by matrix assisted laser desorption/ionization time of flight mass spectrometry (MAIDI-TOF-MS) after irradiation.The mRNA expressions of proteins were analyzed by real-time quantity PCR (RTQ-PCR).Results Using 2-DE and MAIDI-TOF-MS,3 kinds of altered proteins were definite in terms of eukaryotic translation initiation factor 5A (eIF5A),profoldin subunit 1 (PFDN1) and fatty acid binding protein 4 (FABP4).The mRNA expressions of eIF5A and FABP4 were coincided with protein expressions.Conclusions These differentially expressed proteins induced by low-doses of γ-rays will help to elucidate the mechanisms of biological effects at low doses and offer the reference for further research of novel biomarkers for low dose exposure.