CAJ | 학술논문

目的探索辐射诱导人肺腺癌细胞STAT3活化的剂量和时间效应及其上游信号通路.方法 60Co γ射线照射p53突变型肺腺癌细胞H322,0～ 10 Gy照射后0～24 h提取蛋白,利用Western blot方法和免疫荧光染色实验,检测H322细胞中磷酸化STAT3表达和入核情况,以及其上游信号分子p-EGFR水平.结果 Western blot方法和免疫荧光染色实验均表明2～10 Gy照射后(1.9 ～6.6倍)0～24 h(1.2 ～9.5倍)均可诱导肺腺癌细胞H322中p-STAT3表达增高;同时发现H322细胞中出现p-EGFR表达的增加,其为对照组的4.3 ～19.2倍.结论 60Co γ射线照射后引起肺腺癌H322细胞中p-STAT3表达增高,STAT3的激活可能由EGFR/STAT3信号通路所介导.
목적 탐색복사유도인폐선암세포STAT3활화적제량화시간효응급기상유신호통로.방법 60Co γ사선조사p53돌변형폐선암세포H322,0～ 10 Gy조사후0～24 h제취단백,이용Western blot방법화면역형광염색실험,검측H322세포중린산화STAT3표체화입핵정황,이급기상유신호분자p-EGFR수평.결과 Western blot방법화면역형광염색실험균표명2～10 Gy조사후(1.9 ～6.6배)0～24 h(1.2 ～9.5배)균가유도폐선암세포H322중p-STAT3표체증고;동시발현H322세포중출현p-EGFR표체적증가,기위대조조적4.3 ～19.2배.결론 60Co γ사선조사후인기폐선암H322세포중p-STAT3표체증고,STAT3적격활가능유EGFR/STAT3신호통로소개도.
Objective To investigate the dose-,time-effect of STAT3 activation induced by radiation and explore the underlying signal pathway.Methods Pulmonary adenocarcinoma cells (H322) with mutated p53 gene were irradiated with 0-10 Gy of γ-rays.After radiation,the expression and transcriptional activity of STAT3 and the levels of its upstream molecules,including p-EGFR,were detected by Western blot.The nucleus translocation of p-STAT3 was examined by immunofluorescence staining assay.Results The protein expression and transcriptional activation of p-STAT3 in H322 cells were increased by 1.9-6.6 fold after 2-10 Gy radiation and increased by 1.2-9.5 fold after 0-24 h of irradiation.In addition,the expression of p-EGFR was also increased by 4.3-19.2 fold after irradiation.Conclusions After radiation,the expression of p-STAT3 in the pulmonary adenocarcinoma H322 cell can be increased probably through EGFR/STAT3 signal pathway.