CAJ | 학술논문

目的探讨micro RNA-122(miRNA-122)的SNP位点与肝细胞癌(HCC)遗传易感性以及术后早期复发的关系.方法采用病例-对照研究设计,提取173例HCC患者石蜡切片组织DNA,PCR扩增,查找miRNA-122包括编码基因在内共357个碱基序列内的SNP位点,分析其与HCC易感性及术后早期复发的关系.结果上述miRNA-122序列中只找到rs17669T＞C位点,基因库中标注的其他7个SNP位点均未发现.rs17669各基因型分布频率:C/C型7例(4.0％)、T/T型110例(63.6％)、C/T型56例(32.4％).C/C基因型相对于T/T基因型为肝癌的保护因素(OR=0.213,95％ CI:0.062-0.732);rs17669各基因型与术后复发无关.结论 rs17669可能与有乙肝背景的江西汉族人群肝癌遗传易感性相关,与术后复发无关.
목적 탐토micro RNA-122(miRNA-122)적SNP위점여간세포암(HCC)유전역감성이급술후조기복발적관계.방법 채용병례-대조연구설계,제취173례HCC환자석사절편조직DNA,PCR확증,사조miRNA-122포괄편마기인재내공357개감기서렬내적SNP위점,분석기여HCC역감성급술후조기복발적관계.결과 상술miRNA-122서렬중지조도rs17669T＞C위점,기인고중표주적기타7개SNP위점균미발현.rs17669각기인형분포빈솔:C/C형7례(4.0％)、T/T형110례(63.6％)、C/T형56례(32.4％).C/C기인형상대우T/T기인형위간암적보호인소(OR=0.213,95％ CI:0.062-0.732);rs17669각기인형여술후복발무관.결론 rs17669가능여유을간배경적강서한족인군간암유전역감성상관,여술후복발무관.
Objective To investigate the association of single nucleotide polymorphism (SNP) in micro RNA-122 (miRNA-122) with genetic predisposition and early recurrence after resection for hepatocellular carcinoma(HCC).Methods This is a case-control study involving 173 HCC patients.DNA were exacted from cancer tissues embedded in paraffin and were amplified by PCR.The study aimed to explore SNP in gene sequence of miRNA-122 (357 base pair including extron.The outcomes of genetic predisposition were analyzed with early recurrence after resection for HCC.Results Only rs17669 was found in miRNA-122.The genetype frequence of C/C,T/T and C/T at rs17669 gene locus were 7(4.0％),110(63.6％)and 56(32.4％),respectively.When compared to T/T genetype,C/C genetype was a protective factor of risk of HCC (OR =0.213,95％ CI:0.062-0.732).Genetypes had no relationship with early recurrence after resection.Conclusion For HCC recurrence,rs17669 may be associated with genetic predisposition of HCC in Hans in Jiangxi infected with HBV.