CAJ | 학술논문

目的探讨沉默信息调节因子2相关酶1(SIRT1)在肝癌中的表达及其临床意义.方法收集252例乙肝后肝癌病例组织标本及随访资料.通过基因测序分析p53突变情况并通过蛋白印迹法检测肝癌组织中SIRT1和腺苷酸活化蛋白激酶(AMPK)的表达情况.结果在p53突变型肝癌(n＝54)中,SIRT1活化的比例较野生型p53肝癌高(64.8％比31.8％;P＜0.01),且活化的SIRT1预示着更高的无复发生存率.多变量分析显示SIRT1是影响无复发生存率的独立因素(OR:0.339,CI:0.160-0.720,P=0.005).SIRT1活性与AMPK活性显著相关(P =0.007).结论在p53突变型肝癌中SIRT1通过AMPK途径表现出抗肿瘤效应.
목적 탐토침묵신식조절인자2상관매1(SIRT1)재간암중적표체급기림상의의.방법 수집252례을간후간암병례조직표본급수방자료.통과기인측서분석p53돌변정황병통과단백인적법검측간암조직중SIRT1화선감산활화단백격매(AMPK)적표체정황.결과 재p53돌변형간암(n＝54)중,SIRT1활화적비례교야생형p53간암고(64.8％비31.8％;P＜0.01),차활화적SIRT1예시착경고적무복발생존솔.다변량분석현시SIRT1시영향무복발생존솔적독립인소(OR:0.339,CI:0.160-0.720,P=0.005).SIRT1활성여AMPK활성현저상관(P =0.007).결론 재p53돌변형간암중SIRT1통과AMPK도경표현출항종류효응.
Objective To investigate the clinical significance of silent mating-type information regulation 2 homologue 1 (SIRT1) in hepatocellular carcinoma (HCC).Method We analyzed p53 mutation by gene sequencing and activation of SIRT1 and AMP-actived protein kinase (AMPK) using western-blot in 252 patients with hepatitis B virus-positive HCC.Results A higher proportion of tissues with mutant p53 were demonstrated to harbor activated SIRT1 (64.8％ vs 31.8％ ; P ＜ 0.01).Activated SIRT1 predicted a longer relapse-free survival.On multivariate analysis,activated SIRT1 remained significant (OR:0.339,CI:0.160-0.720,P =0.005).Analysis of 252 paired specimens revealed a significant correlation between activated SIRT1 and activated AMPK in HCC tissues harboring mutant p53 (P =0.007).Conclusion SIRT1 exerted anti-carcinogenic effects through the AMPK pathway in HCC in the context of mutant p53.