中华精神科杂志
中華精神科雜誌
중화정신과잡지
CHINESE JOURNA OF PSYCHIATRY
2014年
6期
347-351
,共5页
姚能云%佘艳梅%周红勤%赵锋%唐普照%刘丽桃%李海军%肖波
姚能雲%佘豔梅%週紅勤%趙鋒%唐普照%劉麗桃%李海軍%肖波
요능운%사염매%주홍근%조봉%당보조%류려도%리해군%초파
精神分裂症%Borna病病毒%p40基因片段%p24基因片段
精神分裂癥%Borna病病毒%p40基因片段%p24基因片段
정신분렬증%Borna병병독%p40기인편단%p24기인편단
Schizophrenia%Borna disease virus%p40 gene%p24gene
目的 探讨博尔纳病毒(Borna disease virus,BDV)感染与精神分裂症的相关性,分析感染BDV的精神分裂症患者临床特征.方法 采用荧光定量巢式逆转录聚合酶链式反应方法检测86例精神分裂症患者(患者组)和84名健康体检者(对照组)外周血单个核细胞中BDVp24和BDVp40基因片段,以β-肌动蛋白作为内参照,将BDVp24和BDVp40基因片段均为阳性的标本基因进行测序分析,外周血进行抗BDV抗体滴度测定,对患者的精神症状采用PANSS进行量化评分,并总结阳性患者的临床特征.结果 86例精神分裂症患者中外周血标本BDV p24基因片段阳性检出率11.6％ (10/86),BDVp40基因片段检出率13.4％ (12/86),拷贝数均＞102 kb/μl.对照组检测BDVp24、BDVp40基因片段均为阴性,两组阳性率比较差异有统计学意义(x2=9.26,P＜0.05).对BDV p24和BDVp40基因片段均为阳性的标本测序后,与马源的BDV病毒株亲缘关系最近.阳性标本的精神分裂症患者精神症状主要以幻觉、妄想等阳性症状为主,PANSS量表评分均＞40分.结论 精神分裂症发病与BDV感染有一定的相关性,是否是唯一的相关性还有待进一步研究,BDV感染的精神分裂症患者主要以阳性精神症状为主.
目的 探討博爾納病毒(Borna disease virus,BDV)感染與精神分裂癥的相關性,分析感染BDV的精神分裂癥患者臨床特徵.方法 採用熒光定量巢式逆轉錄聚閤酶鏈式反應方法檢測86例精神分裂癥患者(患者組)和84名健康體檢者(對照組)外週血單箇覈細胞中BDVp24和BDVp40基因片段,以β-肌動蛋白作為內參照,將BDVp24和BDVp40基因片段均為暘性的標本基因進行測序分析,外週血進行抗BDV抗體滴度測定,對患者的精神癥狀採用PANSS進行量化評分,併總結暘性患者的臨床特徵.結果 86例精神分裂癥患者中外週血標本BDV p24基因片段暘性檢齣率11.6％ (10/86),BDVp40基因片段檢齣率13.4％ (12/86),拷貝數均＞102 kb/μl.對照組檢測BDVp24、BDVp40基因片段均為陰性,兩組暘性率比較差異有統計學意義(x2=9.26,P＜0.05).對BDV p24和BDVp40基因片段均為暘性的標本測序後,與馬源的BDV病毒株親緣關繫最近.暘性標本的精神分裂癥患者精神癥狀主要以幻覺、妄想等暘性癥狀為主,PANSS量錶評分均＞40分.結論 精神分裂癥髮病與BDV感染有一定的相關性,是否是唯一的相關性還有待進一步研究,BDV感染的精神分裂癥患者主要以暘性精神癥狀為主.
목적 탐토박이납병독(Borna disease virus,BDV)감염여정신분렬증적상관성,분석감염BDV적정신분렬증환자림상특정.방법 채용형광정량소식역전록취합매련식반응방법검측86례정신분렬증환자(환자조)화84명건강체검자(대조조)외주혈단개핵세포중BDVp24화BDVp40기인편단,이β-기동단백작위내삼조,장BDVp24화BDVp40기인편단균위양성적표본기인진행측서분석,외주혈진행항BDV항체적도측정,대환자적정신증상채용PANSS진행양화평분,병총결양성환자적림상특정.결과 86례정신분렬증환자중외주혈표본BDV p24기인편단양성검출솔11.6％ (10/86),BDVp40기인편단검출솔13.4％ (12/86),고패수균＞102 kb/μl.대조조검측BDVp24、BDVp40기인편단균위음성,량조양성솔비교차이유통계학의의(x2=9.26,P＜0.05).대BDV p24화BDVp40기인편단균위양성적표본측서후,여마원적BDV병독주친연관계최근.양성표본적정신분렬증환자정신증상주요이환각、망상등양성증상위주,PANSS량표평분균＞40분.결론 정신분렬증발병여BDV감염유일정적상관성,시부시유일적상관성환유대진일보연구,BDV감염적정신분렬증환자주요이양성정신증상위주.
Objective To explore correlation between Borna disease virus (BDV) infection and schizophrenia,as well as the clinical features of patients with BDV infection.Methods The p24 and p40 gene fragments of BDV in 86 patients with schizophrenia and 84 healthy donors were examined in peripheral blood mononuclear cells (PBMCs) and cerebrospinal fluid mononuclear cells nested with fluorescence quantitative nested reverse transcriptase polymerase chain reaction (FQ-nRT-PCR).Meanwhile,β-actin in the specimens were detected by PCR as internal reference,and genetic testing and BDV antibody titer in patients with schizophrenia infected by BDV were detected.The clinical characteristics of patients infected by BDV were summarized.Results There were 10 patients with schizophrenia infected by BDV with the positive rate of 11.6％ (10/86) and 13.4％ (12/86) of BDV p24 and p40 in PBMCs which were significantly higher than that in healthy donors (x2 =9.26,P ＜ 0.05),and the number of copies of BDV p24 and p40 in PBMCs were more than 102 kb/μl.The homology of the gene sequence of positive product was genetically relative to the virus strain BDV from horse.The clinical features in patients with BDV positive were mainly hallucination and delusion.Conclusion The results suggest that BDV infection possibly exists in some schizophrenia patients.The patients with BDV infection seem to have positive symptom.
