中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2014年
12期
742-747
,共6页
菌斑微生物%16SrDNA%PCR-DGGE%克隆测序
菌斑微生物%16SrDNA%PCR-DGGE%剋隆測序
균반미생물%16SrDNA%PCR-DGGE%극륭측서
Dental plaque%16SrDNA%Polymerase chain reaction-denaturing gradient gel electrophoresis%Cloning and sequencing
目的 探讨有龋和无龋成年人龈上菌斑中微生物的组成特点及多样性差异,以期为龋病的病因学研究提供依据.方法 有龋组选择2013年7月至9月于首都医科大学口腔医学院牙体牙髓科就诊的患者,男女各8例,年龄18～ 35岁,龋失补牙数(decayed tooth and decayedmissing-filled-tooth,DMFT) ≥6且龋齿数≥3;无龋组选择同年龄段DMFT=0的知情同意者,男女各8名.每组16例.分别采集两组受试者龈上菌斑样本,应用PCR-变性梯度凝胶电泳(PCR-denaturing gradient gel electrophoresis,PCR-DGGE)联合克隆测序法对细菌组成进行研究.结果 两组共检测到6个菌门、28个菌属、88个菌种.有龋组中普氏菌属、二氧化碳噬纤维菌属、放线菌属、韦荣菌属及棒状杆菌属的检出率较高,这5个菌属克隆数的总和占有龋组总克隆数的56.2％(334/594).与有龋组相比,无龋组拥有更丰富的优势菌属组合,普氏菌属、韦荣菌属、二氧化碳噬纤维菌属、棒状杆菌属、链球菌属、放线菌属、Aggregatibacter菌属和奈瑟菌属的检出率较高,8个菌属的克隆总数占无龋组总克隆数的65.2％(354/543).有龋组微生物在菌种分类水平上的各个多样性指数均显著低于无龋组,差异有统计学意义 (P＜0.05).结论 龋病的发生并非由单一菌种所致,可能为多种细菌共同作用的结果;随着龋病的发展,龈上菌斑中的微生物多样性下降.
目的 探討有齲和無齲成年人齦上菌斑中微生物的組成特點及多樣性差異,以期為齲病的病因學研究提供依據.方法 有齲組選擇2013年7月至9月于首都醫科大學口腔醫學院牙體牙髓科就診的患者,男女各8例,年齡18～ 35歲,齲失補牙數(decayed tooth and decayedmissing-filled-tooth,DMFT) ≥6且齲齒數≥3;無齲組選擇同年齡段DMFT=0的知情同意者,男女各8名.每組16例.分彆採集兩組受試者齦上菌斑樣本,應用PCR-變性梯度凝膠電泳(PCR-denaturing gradient gel electrophoresis,PCR-DGGE)聯閤剋隆測序法對細菌組成進行研究.結果 兩組共檢測到6箇菌門、28箇菌屬、88箇菌種.有齲組中普氏菌屬、二氧化碳噬纖維菌屬、放線菌屬、韋榮菌屬及棒狀桿菌屬的檢齣率較高,這5箇菌屬剋隆數的總和佔有齲組總剋隆數的56.2％(334/594).與有齲組相比,無齲組擁有更豐富的優勢菌屬組閤,普氏菌屬、韋榮菌屬、二氧化碳噬纖維菌屬、棒狀桿菌屬、鏈毬菌屬、放線菌屬、Aggregatibacter菌屬和奈瑟菌屬的檢齣率較高,8箇菌屬的剋隆總數佔無齲組總剋隆數的65.2％(354/543).有齲組微生物在菌種分類水平上的各箇多樣性指數均顯著低于無齲組,差異有統計學意義 (P＜0.05).結論 齲病的髮生併非由單一菌種所緻,可能為多種細菌共同作用的結果;隨著齲病的髮展,齦上菌斑中的微生物多樣性下降.
목적 탐토유우화무우성년인간상균반중미생물적조성특점급다양성차이,이기위우병적병인학연구제공의거.방법 유우조선택2013년7월지9월우수도의과대학구강의학원아체아수과취진적환자,남녀각8례,년령18～ 35세,우실보아수(decayed tooth and decayedmissing-filled-tooth,DMFT) ≥6차우치수≥3;무우조선택동년령단DMFT=0적지정동의자,남녀각8명.매조16례.분별채집량조수시자간상균반양본,응용PCR-변성제도응효전영(PCR-denaturing gradient gel electrophoresis,PCR-DGGE)연합극륭측서법대세균조성진행연구.결과 량조공검측도6개균문、28개균속、88개균충.유우조중보씨균속、이양화탄서섬유균속、방선균속、위영균속급봉상간균속적검출솔교고,저5개균속극륭수적총화점유우조총극륭수적56.2％(334/594).여유우조상비,무우조옹유경봉부적우세균속조합,보씨균속、위영균속、이양화탄서섬유균속、봉상간균속、련구균속、방선균속、Aggregatibacter균속화내슬균속적검출솔교고,8개균속적극륭총수점무우조총극륭수적65.2％(354/543).유우조미생물재균충분류수평상적각개다양성지수균현저저우무우조,차이유통계학의의 (P＜0.05).결론 우병적발생병비유단일균충소치,가능위다충세균공동작용적결과;수착우병적발전,간상균반중적미생물다양성하강.
Objective To investigate the microbial composition and differences in dental plaque of caries and caries-free adults.Methods Caries-active(n=16) was defined as an individual who had at least three untreated decayed tooth and decayed-missing-filled-tooth(DMFT) score greater than 6.Caries-free (n=16) was defined as an individual who had a DMFT score equal to zero.The patients were 18-35 years old.Samples from supra-gingival plaque were obtained and PCR-denaturing gel electrophoresis cloning and sequencing of caries pathogens were used to catch the core microbial of dental caries.Results Six phylum,28 genus and 88 species were detected.In caries group,Prevotella,Capnocytophaga,Actinomyces,Veillonella and Corynebacterium were predominant,accounting for 56.2％ (334/594) of the total cloning number of caries group.Caries-free group contained more predonminant genus than caries group.Prevotella,Veillonella,Capnocytophaga,Corynebacterium,Streptococcus,Actinomyces,Aggregatibacter and Neisseria were predominant,accounting for 65.2％ (354/543) of the total cloning number of caries-free group.Caries group had less diversity than the caries-free group.The difference was statistically significant(P＜0.05).Conclusions Caries might be caused by potentially pathogenic microbial communities rather than a single pathogen.In the progress of dental caries,the microbial diversity decreased.
