中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2014年
12期
748-752
,共5页
陈宇%辛隐子%刘楚峰%陈奕嘉%曹阳
陳宇%辛隱子%劉楚峰%陳奕嘉%曹暘
진우%신은자%류초봉%진혁가%조양
牙正畸牵引%扣带回%大脑皮质%蛋白激酶Mζ
牙正畸牽引%釦帶迴%大腦皮質%蛋白激酶Mζ
아정기견인%구대회%대뇌피질%단백격매Mζ
Orthodontic extrusion%Gyrus cinguli%Cerebral cortex%Protein kinases Mζ
目的 探讨不同正畸力值作用下大鼠大脑前扣带回皮质层蛋白激酶Mζ (protein kinasesMζ,PKMζ)表达水平的变化,进一步探讨中枢神经系统的突触可塑性在牙移动疼痛过程中的调控作用.方法 选用200 ～ 250 g雄性SD大鼠136只,使用随机数字表法随机分为加力组(96只)、阴性对照组(20只)和空白对照组(20只).加力组大鼠安装实验性牙移动装置并分别施加0.39、0.78、1.17 N正畸力(每个加力亚组各32只),阴性对照组安装相同装置但不加力,空白对照组不予任何处理.观察、记录各组大鼠挠嘴时间,并于术后3d取大脑前扣带回皮质组织(3个加力亚组及两个对照组各20只),免疫组织化学法和酶联免疫吸附测定法检测PKMζ的蛋白表达水平.术后3d向各加力亚组大鼠前扣带回皮质区域分别注射生理盐水和PKMζ抑制剂(ζ-pseudosubstrate inhibitory peptide,ZIP)(每加力亚组12只),观察大鼠挠嘴时间的变化.结果 两个对照组挠嘴时间、免疫组化吸光度值和PKMζ蛋白表达差异均无统计学意义(P＞0.05).随正畸力值的增大,0.39、0.78、1.17 N加力亚组大鼠的挠嘴时间[(58.6±6.9)、(66.3±7.8)、(78.9±8.7)s]、免疫组化吸光度值(4 569±454、6 850±365、8 294±558)和PKMζ蛋白表达水平[(0.32±0.02)、(0.34±0.02)、(0.36±0.02) mg/L]均逐渐升高,3个加力亚组间、各加力亚组与空白对照组、阴性对照组间的差异均有统计学意义(P＜0.05).注射生理盐水前后各加力亚组大鼠挠嘴时间差异均无统计学意义(P＞0.05);注射ZIP后各加力亚组大鼠挠嘴时间与注射前相比均显著减少(P＜0.01).结论 正畸力值增大引起大鼠疼痛程度的增加,可能与大脑前扣带回皮质层PKMζ表达水平升高相关.
目的 探討不同正畸力值作用下大鼠大腦前釦帶迴皮質層蛋白激酶Mζ (protein kinasesMζ,PKMζ)錶達水平的變化,進一步探討中樞神經繫統的突觸可塑性在牙移動疼痛過程中的調控作用.方法 選用200 ～ 250 g雄性SD大鼠136隻,使用隨機數字錶法隨機分為加力組(96隻)、陰性對照組(20隻)和空白對照組(20隻).加力組大鼠安裝實驗性牙移動裝置併分彆施加0.39、0.78、1.17 N正畸力(每箇加力亞組各32隻),陰性對照組安裝相同裝置但不加力,空白對照組不予任何處理.觀察、記錄各組大鼠撓嘴時間,併于術後3d取大腦前釦帶迴皮質組織(3箇加力亞組及兩箇對照組各20隻),免疫組織化學法和酶聯免疫吸附測定法檢測PKMζ的蛋白錶達水平.術後3d嚮各加力亞組大鼠前釦帶迴皮質區域分彆註射生理鹽水和PKMζ抑製劑(ζ-pseudosubstrate inhibitory peptide,ZIP)(每加力亞組12隻),觀察大鼠撓嘴時間的變化.結果 兩箇對照組撓嘴時間、免疫組化吸光度值和PKMζ蛋白錶達差異均無統計學意義(P＞0.05).隨正畸力值的增大,0.39、0.78、1.17 N加力亞組大鼠的撓嘴時間[(58.6±6.9)、(66.3±7.8)、(78.9±8.7)s]、免疫組化吸光度值(4 569±454、6 850±365、8 294±558)和PKMζ蛋白錶達水平[(0.32±0.02)、(0.34±0.02)、(0.36±0.02) mg/L]均逐漸升高,3箇加力亞組間、各加力亞組與空白對照組、陰性對照組間的差異均有統計學意義(P＜0.05).註射生理鹽水前後各加力亞組大鼠撓嘴時間差異均無統計學意義(P＞0.05);註射ZIP後各加力亞組大鼠撓嘴時間與註射前相比均顯著減少(P＜0.01).結論 正畸力值增大引起大鼠疼痛程度的增加,可能與大腦前釦帶迴皮質層PKMζ錶達水平升高相關.
목적 탐토불동정기력치작용하대서대뇌전구대회피질층단백격매Mζ (protein kinasesMζ,PKMζ)표체수평적변화,진일보탐토중추신경계통적돌촉가소성재아이동동통과정중적조공작용.방법 선용200 ～ 250 g웅성SD대서136지,사용수궤수자표법수궤분위가력조(96지)、음성대조조(20지)화공백대조조(20지).가력조대서안장실험성아이동장치병분별시가0.39、0.78、1.17 N정기력(매개가력아조각32지),음성대조조안장상동장치단불가력,공백대조조불여임하처리.관찰、기록각조대서뇨취시간,병우술후3d취대뇌전구대회피질조직(3개가력아조급량개대조조각20지),면역조직화학법화매련면역흡부측정법검측PKMζ적단백표체수평.술후3d향각가력아조대서전구대회피질구역분별주사생리염수화PKMζ억제제(ζ-pseudosubstrate inhibitory peptide,ZIP)(매가력아조12지),관찰대서뇨취시간적변화.결과 량개대조조뇨취시간、면역조화흡광도치화PKMζ단백표체차이균무통계학의의(P＞0.05).수정기력치적증대,0.39、0.78、1.17 N가력아조대서적뇨취시간[(58.6±6.9)、(66.3±7.8)、(78.9±8.7)s]、면역조화흡광도치(4 569±454、6 850±365、8 294±558)화PKMζ단백표체수평[(0.32±0.02)、(0.34±0.02)、(0.36±0.02) mg/L]균축점승고,3개가력아조간、각가력아조여공백대조조、음성대조조간적차이균유통계학의의(P＜0.05).주사생리염수전후각가력아조대서뇨취시간차이균무통계학의의(P＞0.05);주사ZIP후각가력아조대서뇨취시간여주사전상비균현저감소(P＜0.01).결론 정기력치증대인기대서동통정도적증가,가능여대뇌전구대회피질층PKMζ표체수평승고상관.
Objective To investigate the regulatory effect of central synaptic plasticity on pain induced by experimental tooth movement and to analyzethe expression of protein kinases Mζ(PKMζ) in the anterior cingulate cortex(ACC) after applying different magnitude of orthodontic force.Methods One hundred and thirty-six male Sprague-Dawley(SD) rats(200-250 g) were used in this study.Orthodontic tooth movement devices were placed on the teeth in the experimental group,and different orthodontic forces(0.39,0.78,1.17 N) were applied to move the maxillary first molars,respectively.The same mechanical devices were placed on the teeth in sham-treated group and no orthodontic force was applied.No orthodontic procedure was applied in blank control group.The average time spent on mouth-wiping behavior in each group was recorded after experimental tooth movement.Brain tissue of the anterior cingulate cortex was isolated on day 3 after experiment,and the expression level of PKMζ was analyzed with the method of immunohistochemistry and enzyme-linked immune sorbent assay.ζ-pseudosubstrate inhibitory peptide(ZIP),a selective inhibitor for PKMζ,was injected into ACC on day 3 after experimental tooth movement,and the effects of ZIP on mouth-wiping behavior were evaluated.Results No statistical difference was found between the blank control group and the sham-treated group in the average time spent on mouth-wiping,value of A and expression level of PKMζ(P＞0.05).Compared with the sham-treated group and blank control group,the average time of mouth-wiping behavior[(58.6±6.9),(66.3±7.8),(78.9±8.7) s],value of A(4 569±454,6 850±365,8 294±558) and expression level of PKMζ[(0.32±0.02),(0.34±0.02),(0.36±0.02) mg/L] in 0.39,0.78,1.17 N force group were found to be up-regulated with the increase of orthodontic force(P＜0.05).LSD test in three experimental sub-group showed statistical difference(P＜0.05).After microinjection of ZIP,the average time spent on mouth-wiping behavior significantly decreased(P＜0.01),while microinjecting saline did not change rats' mouth-wiping behavior(P＞0.05).Conclusions More pain caused by increased orthodontic force maybe due to the up-regulation of PKMζ in the anterior cingulate cortex.
