CAJ | 학술논문

目的探讨纳米硫化镉(nano-CdS)对雄性小鼠生殖系统的影响.方法 36只SPF级雄性ICR小鼠随机均分3组,每组12只.经口染毒nano-CdS(100或50 mg/kg),每天染毒1次,对照组灌胃等体积生理盐水.检测睾丸组织中镉元素积累水平和血清睾酮水平,显微镜下观察附睾精子数量、畸形率;睾丸作病理切片进行观察,采用实时荧光定量PCR检测CYP11A1 mRNA水平.结果与对照组相比,实验组睾丸镉元素含量显著增加,100mg/kg nano-CdS组附睾精子数显著降低,精子畸形率增高,差异有统计学意义(P＜0.05).而50mg/kg nano-CdS组未见明显改变.100 mg/kg nano-CdS组睾丸的病理切片观察到不同程度的损伤,50 mg/kg nano-CdS组未见明显病理变化;实验组睾酮水平,CYP11A1mRNA水平相较于对照组明显降低.结论通过消化道染毒nano-CdS对雄性小鼠生殖系统可能产生影响.其作用机制为nano-CdS对雄激素合成通路中的关键酶基因造成影响,导致生殖激素水平降低.
목적 탐토납미류화력(nano-CdS)대웅성소서생식계통적영향.방법 36지SPF급웅성ICR소서수궤균분3조,매조12지.경구염독nano-CdS(100혹50 mg/kg),매천염독1차,대조조관위등체적생리염수.검측고환조직중력원소적루수평화혈청고동수평,현미경하관찰부고정자수량、기형솔;고환작병리절편진행관찰,채용실시형광정량PCR검측CYP11A1 mRNA수평.결과 여대조조상비,실험조고환력원소함량현저증가,100mg/kg nano-CdS조부고정자수현저강저,정자기형솔증고,차이유통계학의의(P＜0.05).이50mg/kg nano-CdS조미견명현개변.100 mg/kg nano-CdS조고환적병리절편관찰도불동정도적손상,50 mg/kg nano-CdS조미견명현병리변화;실험조고동수평,CYP11A1mRNA수평상교우대조조명현강저.결론 통과소화도염독nano-CdS대웅성소서생식계통가능산생영향.기작용궤제위nano-CdS대웅격소합성통로중적관건매기인조성영향,도치생식격소수평강저.
Objective To investigate the effects of nanosized cadmium sulfide (nano-CdS) on the male reproductive system in mice.Methods Thirty-six specific pathogen-free male ICR mice were equally and randomly divided into three groups:two experimental groups and a control group.The two experimental groups were exposed to 100 mg/kg and 50 mg/kg nano-CdS once daily by gavage,respectively,while the control group was exposed to the same volume of physiological saline once daily by gavage.After 45 days,levels of cadmium accumulation and serum testosterone in the testis were determined,the epididymal sperm count,the rate of sperm abnormality,and histopathological changes in testis tissue were observed under a microscope,and the level of CYP11A1 mRNA was determined by qRT-PCR.Results Compared with the control group,the two experimental groups had a significantly increased level of cadmium accumulation in the testis (P＜0.05),and the 100 mg/kg nano-CdS group had a significantly decreased epididymal sperm count (P＜0.05) and a significantly increased rate of sperm abnormality (P＜0.05),but the 50 mg/kg nano-CdS group did not.The 100 mg/kg nanoCdS group showed different histopathological changes in testis tissue,but the 50 mg/kg nano-CdS group did not.The two experimental groups had significantly reduced levels of testosterone and CYP11A1 mRNA compared with the control group.Conclusion Nano-CdS given through the digestive tract may have an effect on the male reproductive system in mice by affecting the key enzyme genes in the androgen synthesis pathway to reduce the levels of reproductive hormones.