中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2014年
12期
1375-1378
,共4页
刘贞艳%房明%胡彬%毕振旺%寇增强%任艳艳%陈保立%毕振强
劉貞豔%房明%鬍彬%畢振旺%寇增彊%任豔豔%陳保立%畢振彊
류정염%방명%호빈%필진왕%구증강%임염염%진보립%필진강
猩红热%链球菌,酿脓%分子分型
猩紅熱%鏈毬菌,釀膿%分子分型
성홍열%련구균,양농%분자분형
Scarlet fever%Streptococcus pyogenes%Molecular types
目的 描述2013年山东省致猩红热及无症状携带者A群链球菌(GAS)分子分型特征,探讨各分型方法与emm型别的关联.方法 自猩红热、无症状携带者咽拭分离获得GAS72株,利用emm、多位点序列分型(MLST)、超抗原(SAg)基因(speA、speC、speG、speH、speI、speJ、smeZ、ssa、speK、speM、speL)检测和脉冲场凝胶电泳(PFGE)对其进行分型检测.结果 72株GAS中,以emm1型(41.67%)和emm12型(56.94%)为主;共检出两种ST型别,其中ST28型31株(43.06%),ST36型41株(56.94%);ST28型与emm1相关(P<0.05),而ST36型与emm12相关(P<0.05);speA、speC、speG、speH、speI、speJ、smeZ、ssa的检出率分别为38.89%、95.83%、97.22%、38.89%、54.17%、41.67%、100.00%、100.00%,speK、speM、speL未检出;emm1型菌株倾向于含有speA、speJ,不含有speH、speI(P<0.05);emm12恰好相反,倾向于含有speH、speI,不含有speA、speJ(P<0.05);PFGE共检出20种型别.结论 2013年山东省致猩红热和无症状携带者中GAS型别分布较为单一,emm型别以emm1和emm12为主;ST型别主要为ST28和ST36,超抗原基因携带以speC、speG、smeZ、ssa为主.各分型方法间存在一定关联.
目的 描述2013年山東省緻猩紅熱及無癥狀攜帶者A群鏈毬菌(GAS)分子分型特徵,探討各分型方法與emm型彆的關聯.方法 自猩紅熱、無癥狀攜帶者嚥拭分離穫得GAS72株,利用emm、多位點序列分型(MLST)、超抗原(SAg)基因(speA、speC、speG、speH、speI、speJ、smeZ、ssa、speK、speM、speL)檢測和脈遲場凝膠電泳(PFGE)對其進行分型檢測.結果 72株GAS中,以emm1型(41.67%)和emm12型(56.94%)為主;共檢齣兩種ST型彆,其中ST28型31株(43.06%),ST36型41株(56.94%);ST28型與emm1相關(P<0.05),而ST36型與emm12相關(P<0.05);speA、speC、speG、speH、speI、speJ、smeZ、ssa的檢齣率分彆為38.89%、95.83%、97.22%、38.89%、54.17%、41.67%、100.00%、100.00%,speK、speM、speL未檢齣;emm1型菌株傾嚮于含有speA、speJ,不含有speH、speI(P<0.05);emm12恰好相反,傾嚮于含有speH、speI,不含有speA、speJ(P<0.05);PFGE共檢齣20種型彆.結論 2013年山東省緻猩紅熱和無癥狀攜帶者中GAS型彆分佈較為單一,emm型彆以emm1和emm12為主;ST型彆主要為ST28和ST36,超抗原基因攜帶以speC、speG、smeZ、ssa為主.各分型方法間存在一定關聯.
목적 묘술2013년산동성치성홍열급무증상휴대자A군련구균(GAS)분자분형특정,탐토각분형방법여emm형별적관련.방법 자성홍열、무증상휴대자인식분리획득GAS72주,이용emm、다위점서렬분형(MLST)、초항원(SAg)기인(speA、speC、speG、speH、speI、speJ、smeZ、ssa、speK、speM、speL)검측화맥충장응효전영(PFGE)대기진행분형검측.결과 72주GAS중,이emm1형(41.67%)화emm12형(56.94%)위주;공검출량충ST형별,기중ST28형31주(43.06%),ST36형41주(56.94%);ST28형여emm1상관(P<0.05),이ST36형여emm12상관(P<0.05);speA、speC、speG、speH、speI、speJ、smeZ、ssa적검출솔분별위38.89%、95.83%、97.22%、38.89%、54.17%、41.67%、100.00%、100.00%,speK、speM、speL미검출;emm1형균주경향우함유speA、speJ,불함유speH、speI(P<0.05);emm12흡호상반,경향우함유speH、speI,불함유speA、speJ(P<0.05);PFGE공검출20충형별.결론 2013년산동성치성홍열화무증상휴대자중GAS형별분포교위단일,emm형별이emm1화emm12위주;ST형별주요위ST28화ST36,초항원기인휴대이speC、speG、smeZ、ssa위주.각분형방법간존재일정관련.
Objective To describe the molecular characteristics of group A Streptococcus (GAS) isolated from patients and asymptomatic carriers of scarlet fever in Shandong province,2013,and to explore the relationships between emm types and other molecular types.Methods 72 strains of GAS were isolated from throat swabs of children with scarlet fever or asymptomatic carriers of GAS.All the strains were typed by emm typing,multilocus sequence typing (MLST),super-antigen (SAg) genes detections and pulsed-field gel electrophoreses (PFGE).Results Among the 72 strains,emml (41.67%) and emm 12 (56.94%) were the most common emm types.Two ST types were found,including ST28 (43.06%) and ST36 (56.94%).Additionally,emm1 was also found correlated to ST28,while emm12 was associated with ST36.Eight super-antigen genes were detected,including smeZ(100.00%),ssa(100.00%),speG(97.22%),speC(95.83%),speL(54.17%),speJ(41.67%),speA (38.89%) and speH (38.89%),while speK,speM,speL were not found (0%).Both speA and speJ genes were detected primarily in emml strains (all P<0.05),while speH and speI genes were not detected in emm 1 strains (all P<0.05).And emml2 strains were inclined to harbor speH and speL (all P<0.05) but not speA or speJ (all P<0.05).Twenty different genotypes were identified by PFGE.Conclusion All the emm types of GAS isolated from scarlet fever patients and asymptomatic carriers in Shandong province 2013 were mainly emm1 and emm12 and carrying speC,speG and smeZ,ssa.ST types mainly exsited in ST28 and ST36.In addition,there were correlations between emm types and super-antigen genes,ST types,PFGE types.