CAJ | 학술논문

目的了解2013年广东省副溶血弧菌暴发与散发分离株的血清型别、抗菌药物耐药性、毒力基因携带情况以及分子分型特征.方法对36株暴发分离株和43株散发分离株进行血清分型、药敏试验以及耐热直接溶血毒素基因(tdh)、耐热相关溶血毒素基因(trh)、GS-PCR和orf8基因的PCR检测,并进行脉冲场凝胶电泳(PFGE)分型.结果 36株暴发分离株全部为O3∶K6血清型,43株散发分离株的优势血清型为O3∶K6型(23株,53.49％).药敏检测结果显示,对氨苄西林(96.20％)和头孢噻吩(40.50％)的耐药率较高;对复方新诺明和氯霉素则高度敏感,敏感性均为100％.多重耐药分析显示,83.33％(30/36)的暴发分离株同时耐受≥3种抗菌药,37.21％(16/43)的散发分离株同时耐受≥3种抗菌药物.毒力基因PCR检测显示,36株暴发分离株均为tdh+ trh-型菌株.86.05％(37/43)的散发分离株为tdh+ trh-型菌株,11.63％(5/43)为tdh-trh+型菌株,仅1株为tdh+ trh+型菌株.暴发分离株全部携带GS-PCR和/或orf8基因,51.16％(22/43)的散发分离株携带GS-PCR和/或orf8基因.PFGE显示,79株副溶血弧菌经NotⅠ酶切后的PFGE图谱可分为3个聚类,32种PFGE型别,相似值为59.8％～100.0％.暴发菌株聚集在同一个聚类中,散发菌株散布在各个聚类中.结论 2013年广东省副溶血弧菌优势血清型为O3∶K6型,菌株对多数抗菌药物仍然比较敏感,但存在多重耐药现象,多数菌株携带tdh基因,大部分O3∶K6型菌株携带GS-PCR和/或orf8基因;PFGE结果提示广东省副溶血弧菌存在遗传多样性.
목적 료해2013년광동성부용혈호균폭발여산발분리주적혈청형별、항균약물내약성、독력기인휴대정황이급분자분형특정.방법 대36주폭발분리주화43주산발분리주진행혈청분형、약민시험이급내열직접용혈독소기인(tdh)、내열상관용혈독소기인(trh)、GS-PCR화orf8기인적PCR검측,병진행맥충장응효전영(PFGE)분형.결과 36주폭발분리주전부위O3∶K6혈청형,43주산발분리주적우세혈청형위O3∶K6형(23주,53.49％).약민검측결과현시,대안변서림(96.20％)화두포새분(40.50％)적내약솔교고;대복방신낙명화록매소칙고도민감,민감성균위100％.다중내약분석현시,83.33％(30/36)적폭발분리주동시내수≥3충항균약,37.21％(16/43)적산발분리주동시내수≥3충항균약물.독력기인PCR검측현시,36주폭발분리주균위tdh+ trh-형균주.86.05％(37/43)적산발분리주위tdh+ trh-형균주,11.63％(5/43)위tdh-trh+형균주,부1주위tdh+ trh+형균주.폭발분리주전부휴대GS-PCR화/혹orf8기인,51.16％(22/43)적산발분리주휴대GS-PCR화/혹orf8기인.PFGE현시,79주부용혈호균경NotⅠ매절후적PFGE도보가분위3개취류,32충PFGE형별,상사치위59.8％～100.0％.폭발균주취집재동일개취류중,산발균주산포재각개취류중.결론 2013년광동성부용혈호균우세혈청형위O3∶K6형,균주대다수항균약물잉연비교민감,단존재다중내약현상,다수균주휴대tdh기인,대부분O3∶K6형균주휴대GS-PCR화/혹orf8기인;PFGE결과제시광동성부용혈호균존재유전다양성.
Objective To study the serotypes,antimicrobial resistance,virulence genes and molecular characteristics of Vibrio parahaemolyticus isolated from outbreaks and sporadic cases in Guangdong,2013.Methods 36 Vibrio parahaemolyticns strains isolated from outbreaks and 43 strains from sporadic cases were sero-typed and tested for antimicrobial resistance.PCR was used to detect for tdh (thermostable direct hemolysin gene),trh (tdh-related hemolysin gene),GS-PCR and orf8 genes.All the samples were analyzed by pulsed-field gel electrophoresis (PFGE).Results 36 isolates from outbreaks were all identified as O3 ∶ K6,and among the 43 sporadic isolates,O3 ∶ K6 (23,53.49％) was the dominant serotype.Vibrio parahaemolyticus isolates showed high resistance rate to ampicillin(96.20％) and cefalotin (40.50％),but were high sensitive to cotrimoxazole(100％) and chloramphenicol (100％).83.33％ (30/36) outbreak isolates were resistant to multi-drugs but only 37.21％ (16/43)of the sporadic isolates showed so.Results from virulence gene detection suggested that all the 36 outbreak isolates belonged to tdh + trh-strains,while 86.05％ (37/43) of the sporadic isolates were tdh + trh-and 11.63％ (5/43) were tdh-trh +.Only one tdh + trh + strain was found.All the outbreak isolates contained GS-PCR and/or orf8 genes,whereas among the sporadic isolates only 51.16％ (22/43) of them carrying the similar genes.Results from PFGE analysis suggested that 79 isolates were discriminated into 3 clusters and 32 different PFGE patterns with the similarity value between 59.8％ and 100.0％.Outbreak isolates seemed to gather in the same cluster,while the sporadic isolates spreading in all the three clusters.Conclusion O3 ∶ K6 was the dominant serotype in Vibrio parahaemolyticus strains isolated in Guangdong,2013.These strains showed high sensitivity to most antibiotics,but with multi-drug resistance.Positive rate of tdh gene was high,and most O3 ∶ K6 strains contained GS-PCR and/or orf8 genes.PFGE analysis revealed genetic diversity was within the Vibrio parahaemolyticus strains in Guangdong.