CAJ | 학술논문

目的探讨小檗碱预处理对大鼠肝脏冷缺血再灌注时肠损伤的影响.方法清洁级健康雄性SD大鼠24只,体重220～ 250 g,采用随机数字表法分为3组(n=8):假手术组(S组)、肝脏冷缺血再灌注组(I/R组)和小檗碱预处理组(B组).B组于术前7d开始经胃灌注小檗碱200 mg/kg,1次/d,连续7d.I/R组给予等量生理盐水.于术后8h时,留取肠组织,计算湿/干重比(W/D比),进行肠组织损伤评分,测定肠组织MDA和SOD水平、细胞色素C、Bax和Bcl-2的表达和凋亡细胞计数.结果与S组比较,I/R组和B组肠组织损伤评分、W/D比、MDA含量和凋亡细胞计数升高,SOD活性降低,细胞色素C和Bax表达上调,Bcl-2表达下调(P＜0.05);与I/R组比较墙组肠组织损伤评分、W/D比、MDA含量和凋亡细胞计数降低,SOD活性升高,细胞色素C和Bax表达下调,Bcl-2表达上调(P＜0.05).结论小檗碱预处理可减轻大鼠肝脏冷缺血再灌注时的肠损伤,机制与抑制脂质过氧化反应和细胞凋亡有关.
목적 탐토소벽감예처리대대서간장랭결혈재관주시장손상적영향.방법 청길급건강웅성SD대서24지,체중220～ 250 g,채용수궤수자표법분위3조(n=8):가수술조(S조)、간장랭결혈재관주조(I/R조)화소벽감예처리조(B조).B조우술전7d개시경위관주소벽감200 mg/kg,1차/d,련속7d.I/R조급여등량생리염수.우술후8h시,류취장조직,계산습/간중비(W/D비),진행장조직손상평분,측정장조직MDA화SOD수평、세포색소C、Bax화Bcl-2적표체화조망세포계수.결과 여S조비교,I/R조화B조장조직손상평분、W/D비、MDA함량화조망세포계수승고,SOD활성강저,세포색소C화Bax표체상조,Bcl-2표체하조(P＜0.05);여I/R조비교장조장조직손상평분、W/D비、MDA함량화조망세포계수강저,SOD활성승고,세포색소C화Bax표체하조,Bcl-2표체상조(P＜0.05).결론 소벽감예처리가감경대서간장랭결혈재관주시적장손상,궤제여억제지질과양화반응화세포조망유관.
Objective To investigate the effect of berberine preconditioning on the intestinal injury caused by liver cold ischemia/reperfusion (I/R) in rats.Methods Twenty-four pathogen-free male Sprague-Dawley rats,weighing 220-250 g,were randomized into 3 groups (n =8 each) using a random number table:sham operation group (S group),I/R group,and berberine preconditioning group (B group).The animals were anesthetized with 5％ chloral hydrate 60 mg/kg.In B group,berberine 200 mg/kg was administered through a gastric tube once a day for 7 consecutive days starting from 7 days before operation.The equal volume of normal saline was given instead of berberine in group Ⅰ/R.At 8 h after operation,the rats were sacrificed and the intestines were harvested for determination of wet/dry lung weight ratio (W/D ratio),levels of malondialdehyde (MDA) and superoxide dismutase (SOD),expression of cytochrome C,Bax and Bcl-2 and apoptotic cell count,and for microscopic examination.Intestinal damage was assessed and scored according to Chiu.Results Compared with group S,Chiu's score,W/D ratio,MDA content and apoptotic cell count were significantly increased,SOD activity was decreased,the expression of cytochrome C and Bax was up-regulated,and the expression of Bcl-2 was downregulated in I/R and B groups.Compared with group I/R,Chiu's score,W/D ratio,MDA content and apoptotic cell count were significantly decreased,SOD activity was increased,the expression of cytochrome C and Bax was down-regulated,and the expression of Bcl-2 was up-regulated in B group.Conclusion Berberine preconditioning can reduce the intestinal injury caused by liver cold I/R in rats,and the mechanism is related to inhibition of lipid peroxidation and cell apoptosis.