CAJ | 학술논문

目的评价右美托咪定对神经病理性痛大鼠脊髓背根神经节神经元c-fos蛋白表达的影响.方法清洁级健康成年雄性SD大鼠72只,体重180～ 240 g,采用随机数字表法分为3组(n=24):假手术组(S组)仅分离坐骨神经但不结扎;神经病理性痛组(NP组)采用结扎坐骨神经的方法制备大鼠神经病理性痛模型;右美托咪定组(Dex组)于结扎坐骨神经后至处死每天腹腔注射右美托咪定50μg/kg,S组和NP组注射等容量生理盐水.于结扎前1 d(To)、结扎后3、7和14 d(T1-3)时测定大鼠机械缩足反应阈(MWT)和热缩足潜伏期(TWL);于T1-3时测定痛阈后各组随机取8只大鼠处死,取L45脊髓背根神经节,采用免疫组化法检测神经元c-fos蛋白的表达.结果与S组比较,NP组和Dex组T1-3时MWT降低,TWL缩短,脊髓背根神经节神经元c-fos蛋白表达上调(P＜0.05);与NP组比较,Dex组T1-3时MWT升高,TWL延长,脊髓背根神经节神经元c-fos蛋白表达下调(P＜0.05).结论右美托咪定可通过抑制脊髓背根神经节神经元c-fos蛋白表达上调减轻大鼠神经病理性痛.
목적 평개우미탁미정대신경병이성통대서척수배근신경절신경원c-fos단백표체적영향.방법 청길급건강성년웅성SD대서72지,체중180～ 240 g,채용수궤수자표법분위3조(n=24):가수술조(S조)부분리좌골신경단불결찰;신경병이성통조(NP조)채용결찰좌골신경적방법제비대서신경병이성통모형;우미탁미정조(Dex조)우결찰좌골신경후지처사매천복강주사우미탁미정50μg/kg,S조화NP조주사등용량생리염수.우결찰전1 d(To)、결찰후3、7화14 d(T1-3)시측정대서궤계축족반응역(MWT)화열축족잠복기(TWL);우T1-3시측정통역후각조수궤취8지대서처사,취L45척수배근신경절,채용면역조화법검측신경원c-fos단백적표체.결과 여S조비교,NP조화Dex조T1-3시MWT강저,TWL축단,척수배근신경절신경원c-fos단백표체상조(P＜0.05);여NP조비교,Dex조T1-3시MWT승고,TWL연장,척수배근신경절신경원c-fos단백표체하조(P＜0.05).결론 우미탁미정가통과억제척수배근신경절신경원c-fos단백표체상조감경대서신경병이성통.
Objective To evaluate the effect of dexmedetomidine on the expression of c-fos protein in the dorsal root ganglion neurons in a rat model of neuropathic pain (NP).Methods Seventy-two adult male SpragueDawley rats,weighing 180-240 g,were randomly divided into 3 groups (n =24 each) using a random number table:sham operation group (group S),group NP,and dexmedetomidine group (group Dex).The animals were anesthetized with intraperitoneal 10％ chloral hydrate 350 mg/kg.The right sciatic nerve was exposed and 4 loose ligatures were placed on the sciatic nerve at 1 mm intervals with 4-0 silk thread in NP and Dex groups.In group Dex,dexmedetomidine 50μg/kg was injected intraperitoneally once a day starting from the end of operation until the animals were sacrificed.The equal volume of normal saline was given instead of dexmedetomidine in S and NP groups.The mechanical paw withdrawal threshold (MWT) and thermal paw withdrawal latency (TWL) were measured at 1 day before ligation (T0,baseline) and 3,7 and 14 days after ligation (T1-3).Eight animals were sacrificed after measurement of pain threshold at T13 and the dorsal root ganglions of the lumbar segments (L44) were removed for detection of c-fos expression (by immuno-histochemistry).Results Compared with group S,MWT was significantly decreased,TWL was shortened,and the expression of c-fos protein was up-regulated at T1-3 in NP and Dex groups.Compared with NP group,MWT was significantly increased,TWL was prolonged,and the expression of c-fos protein was down-regulated at T1-3 in Dex group.Conclusion Dexmedetomidine can inhibit upregulation of c-fos protein expression,thus attenuating NP in rats.