小剂量氯胺酮对抑郁大鼠无抽搐电休克抗抑郁治疗起效时间和治疗疗程的影响
소제량록알동대억욱대서무추휵전휴극항억욱치료기효시간화치료료정적영향
Effect of small-dose ketamine on onset time and course of modified electroconvulsive therapy in mentally depressed rats
  • 万方数据
    中华麻醉学杂志 중화마취학잡지
    CHINESE JOURNAL OF ANESTHESIOLOGY
    2014年 11期 1365-1368 ,共4页

    陈婧%闵苏%罗洁%彭丽桦%律峰%黎平%郝学超

    진청%민소%라길%팽려화%률봉%려평%학학초

    抑郁%氯胺酮%二异丙酚%电惊厥疗法 抑鬱%氯胺酮%二異丙酚%電驚厥療法
    억욱%록알동%이이병분%전량궐요법
    Depression%Ketamine%Propofol%Electroconvulsive therapy
    目的 评价小剂量氯胺酮对抑郁大鼠无抽搐电休克抗抑郁治疗起效时间和治疗疗程的影响.方法 选择SPF级成年雄性SD大鼠60只,体重220～250 g,2～3月龄.采用随机数字表法,将其分为6组(n=10):正常对照组(C组)、抑郁组(D组)、单纯电休克治疗(ECT)组(ECT组)、异丙酚+ ECT组(PE组)、氯胺酮+ECT组(KE组)和氯胺酮+异丙酚+ECT组(KPE组).采用慢性不可预见性轻度应激法建立抑郁模型.建模后,C组、D组和ECT组腹腔注射生理盐水8 ml/kg; PE组和KE组分别腹腔注射异丙酚100 mg/kg或氯胺酮10 mg/kg; KPE组腹腔注射氯胺酮10 mg/kg和异丙酚80mg/kg.除C组和D组外,其余各组待大鼠翻正反射消失后行ECT(电流50 mA、频率50 Hz、正弦波、脉冲宽度0.7 ms、持续1 s),以引起强直-阵挛抽搐发作为处理成功,ECT 1次/d,连续7d.于建模前、建模后1d及每次ECT结束后(T08)行旷场实验,记录水平活动距离与直立次数.于建模后2d和治疗结束后1d行Morris水迷宫实验,记录逃避潜伏期和目标象限停留时间.结果 与C组比较,D组、ECT组、PE组、KE组T1-8时和KPE组L5时水平活动距离与直立次数减少,逃避潜伏期延长,目标象限停留时间缩短(P＜0.05),KPE组T6.8时水平活动距离、直立次数及治疗后逃避潜伏期与目标象限停留时间差异无统计学意义(P＞0.05);与D组比较,ECT组和PE组T6-8时、KE组及KPE组T4-8时水平活动距离与直立次数增加,ECT组逃避潜伏期延长,目标象限停留时间缩短,KPE组逃避潜伏期缩短,目标象限停留时间延长(P＜0.05);与ECT组和PE组比较,KE组T47时、KPE组T4-8时水平活动距离与直立次数增加,KPE组逃避潜伏期缩短,目标象限停留时间延长(P＜0.05);与KE组比较,KPE组T6-7时水平活动距离与直立次数增加,逃避潜伏期缩短,目标象限停留时间延长(P＜0.05).结论 小剂量氯胺酮可缩短抑郁大鼠无抽搐电休克抗抑郁治疗的起效时间和治疗疗程.
    목적 평개소제량록알동대억욱대서무추휵전휴극항억욱치료기효시간화치료료정적영향.방법 선택SPF급성년웅성SD대서60지,체중220～250 g,2～3월령.채용수궤수자표법,장기분위6조(n=10):정상대조조(C조)、억욱조(D조)、단순전휴극치료(ECT)조(ECT조)、이병분+ ECT조(PE조)、록알동+ECT조(KE조)화록알동+이병분+ECT조(KPE조).채용만성불가예견성경도응격법건립억욱모형.건모후,C조、D조화ECT조복강주사생리염수8 ml/kg; PE조화KE조분별복강주사이병분100 mg/kg혹록알동10 mg/kg; KPE조복강주사록알동10 mg/kg화이병분80mg/kg.제C조화D조외,기여각조대대서번정반사소실후행ECT(전류50 mA、빈솔50 Hz、정현파、맥충관도0.7 ms、지속1 s),이인기강직-진련추휵발작위처리성공,ECT 1차/d,련속7d.우건모전、건모후1d급매차ECT결속후(T08)행광장실험,기록수평활동거리여직립차수.우건모후2d화치료결속후1d행Morris수미궁실험,기록도피잠복기화목표상한정류시간.결과 여C조비교,D조、ECT조、PE조、KE조T1-8시화KPE조L5시수평활동거리여직립차수감소,도피잠복기연장,목표상한정류시간축단(P＜0.05),KPE조T6.8시수평활동거리、직립차수급치료후도피잠복기여목표상한정류시간차이무통계학의의(P＞0.05);여D조비교,ECT조화PE조T6-8시、KE조급KPE조T4-8시수평활동거리여직립차수증가,ECT조도피잠복기연장,목표상한정류시간축단,KPE조도피잠복기축단,목표상한정류시간연장(P＜0.05);여ECT조화PE조비교,KE조T47시、KPE조T4-8시수평활동거리여직립차수증가,KPE조도피잠복기축단,목표상한정류시간연장(P＜0.05);여KE조비교,KPE조T6-7시수평활동거리여직립차수증가,도피잠복기축단,목표상한정류시간연장(P＜0.05).결론 소제량록알동가축단억욱대서무추휵전휴극항억욱치료적기효시간화치료료정.
    Objective To evaluate the effect of small-dose ketamine on the onset time and course of modified electroconvulsive therapy (MECT) in mentally depressed rats.Methods Sixty SPF adult male SpragueDawley rats,aged 2-3 months,weighing 220-250 g,were randomly divided into 6 groups (n =10 each) using a random number table:normal control group (group C),depression group (group D),ECT group,propofol + ECT group (group PE),ketamine + ECT group (group KE) and ketamine + propofol + ECT group (group KPE).The depression model was established by chronic unpredictable mild stress (CUMS).Mter CUMS,C,D and ECT groups received intraperitoneal normal saline 8 ml/kg,group PE received intraperitoneal propofol 100 ml/kg,group KE received intraperitoneal ketamine 10 ml/kg,and group KPE received intraperitoneal ketamine 10 ml/kg + propofol 80 ml/kg.All the groups received ECT once a day for 7 consecutive days starting from the time point when righting reflex was lost except C and D groups.Open-field test was performed before CUMS,at 1 day after CUMS and at the end of each ECT (T0 8).The total distance and the number of standing on the back legs were recorded.Morris water maze test was performed at 2 days after CUMS and 1 day after the end of therapy,and the escape latency and time of staying at the original platform quadrant were recorded.Results Compared with group C,the total distance was shortened and the number of standing on the back legs was reduced,the escape latency was prolonged,and the time of staying at the original platform quadrant was shortened at T1-8 in D,ECT,PE and KE groups and at T1 5 in KPE group,and no significant was found in KPE group in the total distance,number of standing on the back legs,escape latency,and time of staying at the original platform quadrant at T6-8.Compared with group D,the total distance was prolonged and the number of standing on the back legs was increased at T6-8 in ECT and PE groups and at T4-8 in KE and KPE groups,the escape latency was prolonged,and the time of staying at the original platform quadrant was shortened in ECT group,and the escape latency was shortened,and the time of staying at the original platform quadrant was prolonged in KPE group.Compared with ECT and PE groups,the total distance was prolonged and the number of standing on the back legs was increased at T4-7 in group KE and at T4-8 in group KPE,and the escape latency was shortened,and the time of staying at the original platform quadrant was prolonged in KPE group.Compared with group KE,the total distance was prolonged and the number of standing on the back legs was increased at T6.7,the escape latency was shortened,and the time of staying at the original platform quadrant was prolonged in KPE group.Conclusion Small-dose ketamine can shorten the onset time and course of MECT in mentally depressed rats.
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