CAJ | 학술논문

目的探讨远隔缺血后处理对大鼠心肌缺血再灌注时脂联素水平的影响.方法健康清洁级雄性SD大鼠57只,8周龄,体重250 ～ 350 g,采用随机数字表法分为3组(n=19):假手术组(S组)、缺血再灌注组(I/R组)和远隔缺血后处理组(RLIP组).采用结扎左冠状动脉30 min后恢复血流再灌注180 min的方法制备心肌缺血再灌注损伤模型.RLIP组于结扎左冠状动脉20 min时实施远隔缺血后处理:结扎双后肢大腿中、上1/3部位10 min恢复血流再灌注.再灌注30、60、120及180 min时记录ST段幅度.于再灌注180 min时,采集颈静脉血样3ml,检测血清CK-MB活性及cTnI浓度;取心肌组织测定心肌梗死体积(IS),采用ELISA法检测心肌组织脂联素含量.结果与S组比较,I/R组和RLIP组再灌注各时点ST段抬高,血清CK-MB活性及cTnI浓度和IS升高,心肌组织脂联素含量降低(P＜0.05);与I/R组比较,RLIP组再灌注各时点ST段抬高程度降低,血清CK-MB活性及cTnⅠ浓度和IS降低,心肌组织脂联素含量升高(P＜0.05).结论远端缺血后处理减轻大鼠心肌缺血再灌注损伤的机制与增加心肌脂联素水平有关.
목적 탐토원격결혈후처리대대서심기결혈재관주시지련소수평적영향.방법 건강청길급웅성SD대서57지,8주령,체중250 ～ 350 g,채용수궤수자표법분위3조(n=19):가수술조(S조)、결혈재관주조(I/R조)화원격결혈후처리조(RLIP조).채용결찰좌관상동맥30 min후회복혈류재관주180 min적방법제비심기결혈재관주손상모형.RLIP조우결찰좌관상동맥20 min시실시원격결혈후처리:결찰쌍후지대퇴중、상1/3부위10 min회복혈류재관주.재관주30、60、120급180 min시기록ST단폭도.우재관주180 min시,채집경정맥혈양3ml,검측혈청CK-MB활성급cTnI농도;취심기조직측정심기경사체적(IS),채용ELISA법검측심기조직지련소함량.결과 여S조비교,I/R조화RLIP조재관주각시점ST단태고,혈청CK-MB활성급cTnI농도화IS승고,심기조직지련소함량강저(P＜0.05);여I/R조비교,RLIP조재관주각시점ST단태고정도강저,혈청CK-MB활성급cTnⅠ농도화IS강저,심기조직지련소함량승고(P＜0.05).결론 원단결혈후처리감경대서심기결혈재관주손상적궤제여증가심기지련소수평유관.
Objective To evaluate the effect of remote limb ischemic postconditioning on the level of adiponectin during myocardial ischemia-reperfusion (I/R) in rats.Methods Fifty-seven male Sprague-Dawley rats,aged 8 weeks,weighing 250-350 g,were randomly divided into 3 groups (n =19 each):sham operation group (group S); group I/R; remote limb ischemic postconditioning group (group RLIP).Myocardial ischemia was induced by occlusion of anterior descending branch of left coronary artery for 30 min followed by 180 min reperfusion in urethane-anesthetized rats.In group S,the anterior descending branch was only exposed but not ligated.The animals underwent 10 min ischemia of bilateral hind limbs starting from 20 min of occlusion of the anterior descending branch,followed by reperfusion in RLIP group.The changes in S-T segment were recorded at 30,60,120 and 180 min of reperfusion.At 180 min of reperfusion,the blood samples were taken from the jugular vein for determination of serum levels of creatine kinase MB (CK-MB) and cardiac troponin Ⅰ (cTnⅠ).The animals were then sacrificed and hearts were removed for measurement of myocardial infarct size (IS) and adiponectin contents in myocardial tissues (by ELISA).Results Compared with group S,S-T segment was significantly elevated at each time point,myocardial IS,and serum cTnⅠ and CK-MB levels were increased,and adiponectin contents were decreased in I/R and RLIP groups.Compared with group I/R,elevation of S-T segment was significantly decreased at each time point,myocardial IS,and serum cTnⅠ and CK-MB levels were decreased,and adiponectin contents were increased in RLIP group.Conclusion The mechanism by which remote limb ischemic postconditioning reduces myocardial I/R injury is related to increased level of adiponectin in the myocardium of rats.