中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2014年
11期
1396-1398
,共3页
二异丙酚%四肢%再灌注损伤%视网膜%缺血后处理
二異丙酚%四肢%再灌註損傷%視網膜%缺血後處理
이이병분%사지%재관주손상%시망막%결혈후처리
Propofol%Extremities%Reperfusion injury%Retina%Ischemic postconditioning
目的 评价缺血后处理联合异丙酚后处理对大鼠肢体缺血再灌注诱发视网膜损伤的影响.方法 选择雄性Wistar大鼠24只,采用随机数字表法,将其分为3组(n=8):假手术组(S组)、肢体缺血再灌注组(LIR组)、异丙酚后处理+缺血后处理+肢体缺血再灌注组(P+ I+ LIR组).采用分离并结扎双侧后肢股动、静脉60 min后恢复灌注的方法建立肢体缺血再灌注模型.P+I+LIR组缺血60 min后再灌注5 min,继之重新缺血5 min,再灌注5 min,循环4次后恢复灌注,并于再灌注同时腹腔输注异丙酚500 μg· kg-1·min-,在缺血期停止输注,循环4次.于再灌注120 min时采集腹主动脉血样,测定血清MDA浓度.摘取眼球取视网膜组织,HE染色后观察病理学结果.结果 与S组比较,LIR组血清MDA浓度升高(P＜0.05),P+ I+ LIR组血清MDA浓度差异无统计学意义(P＞0.05);与LIR组比较,P+I+LIR组血清MDA浓度降低(P＜0.05).S组视网膜结构正常,LIR组视网膜全层水肿,节细胞层大量空泡变性,结构紊乱;P+ I+ LIR组视网膜病理学改变显著减轻.结论 缺血后处理联合异丙酚后处理可减轻大鼠肢体缺血再灌注诱发视网膜损伤,其机制与抑制脂质过氧化反应有关.
目的 評價缺血後處理聯閤異丙酚後處理對大鼠肢體缺血再灌註誘髮視網膜損傷的影響.方法 選擇雄性Wistar大鼠24隻,採用隨機數字錶法,將其分為3組(n=8):假手術組(S組)、肢體缺血再灌註組(LIR組)、異丙酚後處理+缺血後處理+肢體缺血再灌註組(P+ I+ LIR組).採用分離併結扎雙側後肢股動、靜脈60 min後恢複灌註的方法建立肢體缺血再灌註模型.P+I+LIR組缺血60 min後再灌註5 min,繼之重新缺血5 min,再灌註5 min,循環4次後恢複灌註,併于再灌註同時腹腔輸註異丙酚500 μg· kg-1·min-,在缺血期停止輸註,循環4次.于再灌註120 min時採集腹主動脈血樣,測定血清MDA濃度.摘取眼毬取視網膜組織,HE染色後觀察病理學結果.結果 與S組比較,LIR組血清MDA濃度升高(P＜0.05),P+ I+ LIR組血清MDA濃度差異無統計學意義(P＞0.05);與LIR組比較,P+I+LIR組血清MDA濃度降低(P＜0.05).S組視網膜結構正常,LIR組視網膜全層水腫,節細胞層大量空泡變性,結構紊亂;P+ I+ LIR組視網膜病理學改變顯著減輕.結論 缺血後處理聯閤異丙酚後處理可減輕大鼠肢體缺血再灌註誘髮視網膜損傷,其機製與抑製脂質過氧化反應有關.
목적 평개결혈후처리연합이병분후처리대대서지체결혈재관주유발시망막손상적영향.방법 선택웅성Wistar대서24지,채용수궤수자표법,장기분위3조(n=8):가수술조(S조)、지체결혈재관주조(LIR조)、이병분후처리+결혈후처리+지체결혈재관주조(P+ I+ LIR조).채용분리병결찰쌍측후지고동、정맥60 min후회복관주적방법건립지체결혈재관주모형.P+I+LIR조결혈60 min후재관주5 min,계지중신결혈5 min,재관주5 min,순배4차후회복관주,병우재관주동시복강수주이병분500 μg· kg-1·min-,재결혈기정지수주,순배4차.우재관주120 min시채집복주동맥혈양,측정혈청MDA농도.적취안구취시망막조직,HE염색후관찰병이학결과.결과 여S조비교,LIR조혈청MDA농도승고(P＜0.05),P+ I+ LIR조혈청MDA농도차이무통계학의의(P＞0.05);여LIR조비교,P+I+LIR조혈청MDA농도강저(P＜0.05).S조시망막결구정상,LIR조시망막전층수종,절세포층대량공포변성,결구문란;P+ I+ LIR조시망막병이학개변현저감경.결론 결혈후처리연합이병분후처리가감경대서지체결혈재관주유발시망막손상,기궤제여억제지질과양화반응유관.
Objective To evaluate the effects of postconditioning with ischema and propofol on retinal injury induced by limb ischemia-reperfusion (LIR) in rats.Methods Twenty-four male Wistar rats were randomly divided into 3 groups (n =8 each) using a random number table:sham operation group (group S),group LIR,and propofol postconditioning + postconditioning + ischemic LIR group (group P + I + LIR).The model of LIR was established by separating and ligating the femoral artery and vein of bilateral hind limbs for 60 min followed by reperfusion.In group P + I + LIR,the animals were subjected to 4 episodes of 60 min ischemia-5 min reperfusion-5 min ischemia-5 min reperfusion and to 4 cycles of propofol 500 μg· kg-1 · min-1 infused intraperitoneally at the same time (during reperfusion)-no propofol infusion during second ischemia.At 120 min of reperfusion,blood samples were collected from the abdominal aorta for determination of serum malondialdehyde (MDA) concentrations.The eyeball was removed and the retina was cut into sections which were stained with H.E.and examined by microscopy.Results Compared with group S,the serum MDA concentration was significantly increased in group LIR,and no significant change was found in the serum MDA concentration in group P + I + LIR.The serum MDA concentration was significantly lower in group P + I + LIR than in group LIR.The structure of retina was normal in group S,oedema was found in all the layers of retina,vacuolar degeneration was observed in the most of ganglionic layers,and the structure disturbance was found in group LIR.The pathological changes of retina were significantly attenuated in group P + Ⅰ + LIR.Conclusion Postconditioning with ischema and propofol can attenuate retinal injury induced by LIR,and the mechanism is related to inhibition of lipid peroxidation in rats.
