CAJ | 학술논문

目的在氯化锂-匹鲁卡品诱发大鼠癫痫持续状态(SE)模型中探讨迷走神经刺激(VNS)对SE的影响. 方法 48只Wistar雄性大鼠按照随机数字表法分为4组:A组(VNS预处理组),注射匹鲁卡品前2h开始VNS刺激;B组(VNS治疗组),注射匹鲁卡品后开始VNS刺激;C组(阴性对照组),行VNS电极植入但不予刺激;D组(模型对照组),不予VNS电极植入;每组12只.采用氯化锂-匹鲁卡品腹腔注射诱发大鼠SE.VNS参数:频率30 Hz,波宽500μs,电流1mA,刺激时间30 s,间隔30 s.观察各组大鼠行为学改变,72h后脑组织切片检测各组海马齿状回Caspase-3表达变化. 结果 (1)A、B组大鼠Ⅳ、Ⅴ级(Racine分级)发作次数明显少于C、D组,A组Ⅴ级发作次数明显少于B组,A组SE潜伏期较其他各组均延长,差异均有统计学意义(P＜0.05).(2)A、B2组大鼠海马齿状回Caspase-3表达量明显低于C、D2组(A值分别为5854.7±856.5、6244.8±806.0;11957.0±1948.1、11543.2±1734.7),差异有统计学意义(P＜0.05). 结论 VNS能够使SE大鼠行为学得到改善,且VNS预处理效果优于治疗性VNS刺激;VNS能够减少SE导致的海马神经元早期凋亡,对海马神经元有保护作用.
목적 재록화리-필로잡품유발대서전간지속상태(SE)모형중탐토미주신경자격(VNS)대SE적영향. 방법 48지Wistar웅성대서안조수궤수자표법분위4조:A조(VNS예처리조),주사필로잡품전2h개시VNS자격;B조(VNS치료조),주사필로잡품후개시VNS자격;C조(음성대조조),행VNS전겁식입단불여자격;D조(모형대조조),불여VNS전겁식입;매조12지.채용록화리-필로잡품복강주사유발대서SE.VNS삼수:빈솔30 Hz,파관500μs,전류1mA,자격시간30 s,간격30 s.관찰각조대서행위학개변,72h후뇌조직절편검측각조해마치상회Caspase-3표체변화. 결과 (1)A、B조대서Ⅳ、Ⅴ급(Racine분급)발작차수명현소우C、D조,A조Ⅴ급발작차수명현소우B조,A조SE잠복기교기타각조균연장,차이균유통계학의의(P＜0.05).(2)A、B2조대서해마치상회Caspase-3표체량명현저우C、D2조(A치분별위5854.7±856.5、6244.8±806.0;11957.0±1948.1、11543.2±1734.7),차이유통계학의의(P＜0.05). 결론 VNS능구사SE대서행위학득도개선,차VNS예처리효과우우치료성VNS자격;VNS능구감소SE도치적해마신경원조기조망,대해마신경원유보호작용.
Objective To study the acute effect of vagus nerve stimulation (VNS) on status epilepticus (SE) in rats.Methods Forty-eight male Wistar rats were randomly divided into 4 groups:Group A (VNS preconditioning group),accepted VNS at 2 h before pilocarpine injection,Group B (VNS treatment group),accepted VNS immediately after pilocarpine injection,Group C (negative control group),implanted with electrode without stimulation,and Group D (SE model group),without electrode implantation (n=1 2).Lithium-pilocarpine was used to kindle the rats to establish SE models.Stimulation parameters used in the procedure of VNS were as follows:frequency,30 Hz; pulse width,0.5 ms; current,1 mA; and duty cycle,on-30 s,off-30 s.Behavior changes were observed and caspase-3 expression in dentate gyrus was detected 72 h after pilocarpine injection.Results (1) The seizure frequencies of grade Ⅳ and Ⅴ (Racine grading) in group A and B were significantly fewer than those in group C and D (P＜0.05); the seizure frequency of grade Ⅴ in group A was significantly fewer than that of group B (P＜0.05); SE latency in group A was significantly longer than that in other groups (P＜0.05).(2) Expressions of caspase-3 in the dentate gyrus of group A and B were significantly lower than those of group C and D (absorbancy values being 5854.7±856.5,6244.8±806.0,11957.0±1948.1 and 11543.2±1734.7,P＜0.05).Conclusion VNS has an acute seizure-suppressing effect on SE in rats and it can also reduce neuronal apoptosis induced by SE in hipocampus.