中华神经医学杂志
中華神經醫學雜誌
중화신경의학잡지
CHINESE JOURNAL OF NEUROMEDICINE
2014年
11期
1107-1111
,共5页
王凌雁%郭少雷%齐铁伟%梁丰%黄正松
王凌雁%郭少雷%齊鐵偉%樑豐%黃正鬆
왕릉안%곽소뢰%제철위%량봉%황정송
脑动静脉畸形%间质细胞衍生因子-1α%CXCR4%低氧诱导因子-1α%血管内皮生长因子
腦動靜脈畸形%間質細胞衍生因子-1α%CXCR4%低氧誘導因子-1α%血管內皮生長因子
뇌동정맥기형%간질세포연생인자-1α%CXCR4%저양유도인자-1α%혈관내피생장인자
Brain arteriovenous malformation%Stromal-cell derived factor-1α%CXCR4%Hypoxia inducible factor-1α%Vascular endothelial growth factor
目的 观察脑动静脉畸形(AVM)病灶内间质细胞衍生因子-1α(SDF-1α)及其受体CXCR4的表达情况,以及SDF-1α与低氧诱导因子-1α(HIF-1α)和血管内皮生长因子(VEGF)表达的关系. 方法 选择中山大学附属第一医院神经外科自2012年1月至2013年12月经手术切除并经病理组织学证实的脑AVM标本共48例,应用免疫组化染色方法检测SDF-1α、CXCR4、HIF-1α和VEGF的表达情况,并对表达强度进行评估.评价SDF-1α与VEGF、HIF-1α表达的相关性,分析出血、术前栓塞和Spetzer-Martin分级对SDF-1α表达的影响. 结果 48例AVM患者SDF-1α和CXCR4阳性表达率分别为100％和83.3％,SDF-1α表达于AVM血管内皮细胞、动脉平滑肌细胞,CXCR4表达于血管内皮细胞以及血管周围间质细胞.SDF-1α与VEGF、HIF-1α表达强度呈正相关,相关系数r分别为0.573、0.473,P值分别为0.000、0.001.SDF-1α表达强度在出血患者和未出血患者之间差异无统计学意义(P＞0.05);栓塞患者SDF-1α表达强度高于未栓塞患者,差异有统计学意义(P＜0.05);不同Spetzer-Martin分级患者之间SDF-1α表达强度差异无统计学意义(P＞0.05). 结论 SDF-1α及其受体CXCR4在脑AVM病灶内广泛表达;栓塞将加强脑AVM病灶内SDF-1α的表达.
目的 觀察腦動靜脈畸形(AVM)病竈內間質細胞衍生因子-1α(SDF-1α)及其受體CXCR4的錶達情況,以及SDF-1α與低氧誘導因子-1α(HIF-1α)和血管內皮生長因子(VEGF)錶達的關繫. 方法 選擇中山大學附屬第一醫院神經外科自2012年1月至2013年12月經手術切除併經病理組織學證實的腦AVM標本共48例,應用免疫組化染色方法檢測SDF-1α、CXCR4、HIF-1α和VEGF的錶達情況,併對錶達彊度進行評估.評價SDF-1α與VEGF、HIF-1α錶達的相關性,分析齣血、術前栓塞和Spetzer-Martin分級對SDF-1α錶達的影響. 結果 48例AVM患者SDF-1α和CXCR4暘性錶達率分彆為100％和83.3％,SDF-1α錶達于AVM血管內皮細胞、動脈平滑肌細胞,CXCR4錶達于血管內皮細胞以及血管週圍間質細胞.SDF-1α與VEGF、HIF-1α錶達彊度呈正相關,相關繫數r分彆為0.573、0.473,P值分彆為0.000、0.001.SDF-1α錶達彊度在齣血患者和未齣血患者之間差異無統計學意義(P＞0.05);栓塞患者SDF-1α錶達彊度高于未栓塞患者,差異有統計學意義(P＜0.05);不同Spetzer-Martin分級患者之間SDF-1α錶達彊度差異無統計學意義(P＞0.05). 結論 SDF-1α及其受體CXCR4在腦AVM病竈內廣汎錶達;栓塞將加彊腦AVM病竈內SDF-1α的錶達.
목적 관찰뇌동정맥기형(AVM)병조내간질세포연생인자-1α(SDF-1α)급기수체CXCR4적표체정황,이급SDF-1α여저양유도인자-1α(HIF-1α)화혈관내피생장인자(VEGF)표체적관계. 방법 선택중산대학부속제일의원신경외과자2012년1월지2013년12월경수술절제병경병리조직학증실적뇌AVM표본공48례,응용면역조화염색방법검측SDF-1α、CXCR4、HIF-1α화VEGF적표체정황,병대표체강도진행평고.평개SDF-1α여VEGF、HIF-1α표체적상관성,분석출혈、술전전새화Spetzer-Martin분급대SDF-1α표체적영향. 결과 48례AVM환자SDF-1α화CXCR4양성표체솔분별위100％화83.3％,SDF-1α표체우AVM혈관내피세포、동맥평활기세포,CXCR4표체우혈관내피세포이급혈관주위간질세포.SDF-1α여VEGF、HIF-1α표체강도정정상관,상관계수r분별위0.573、0.473,P치분별위0.000、0.001.SDF-1α표체강도재출혈환자화미출혈환자지간차이무통계학의의(P＞0.05);전새환자SDF-1α표체강도고우미전새환자,차이유통계학의의(P＜0.05);불동Spetzer-Martin분급환자지간SDF-1α표체강도차이무통계학의의(P＞0.05). 결론 SDF-1α급기수체CXCR4재뇌AVM병조내엄범표체;전새장가강뇌AVM병조내SDF-1α적표체.
Objective To investigate the expressions ofstromal-cell derived factor-1α (SDF-1α)and its receptor CXCR4 in brain arteriovenous malformation (AVM) and to explore the relationships of SDF-1α with hypoxia inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF).Methods Surgical specimens from 48 patients accepted brain AVM resection,collected in our hospital from January 2012 to December 2013,were studied for expressions ofSDF-1α,CXCR4,VEGF and HIF-1α by immunohistochemical staining.The relationships of SDF-1α with VEGF and HIF-1α were analyzed and influences of embolism,hemorrhage and Spetzer-Martin classification in SDF-1α expression were assessed.Results SDF-1α and CXCR4 expressed in 100％ and 83.3％ AVM specimens,respectively.The positive staining for SDF-1α was observed in the cytoplasm of vascular endothelium within the nidus and smooth muscle cells of vascular wall.CXCR4 expressed in vascular endothelium and perivascular cells located in the space between the abnormal vessels.SDF-1α expression was significantly associated with VEGF and HIF-1α (r=0.537 and 0.437,respectively,P＜0.05).SDF-1α showed more intense expression in embolized patients than that in non-embolized patients (P＜ 0.05),while no significant difference was noted between patients with and without hemorrhage and between patients of different Spetzer-Martin classifications (P＞0.05).Conclusion SDF-1α and its receptor CXCR4 highly express in brain AVM; preoperative embolization might induce expression of SDF-1α.
