CAJ | 학술논문

目的观察11 mHz超低频经颅磁刺激(ILF-TMS)对大鼠局部脑缺血再灌注损伤模型海马区巢蛋白(nestin)、5-脱氧尿嘧啶核苷(Brdu)表达的影响. 方法大鼠按随机数字表法分为3组,分别为11 mHz组、假刺激组和假手术组,每组24只.11 mHz组、假刺激组参照Longa等的线栓法建立大鼠右侧脑缺血再灌注损伤模型.假手术组麻醉大鼠后,钝性分离各血管但不结扎,亦不进线.11mHz组给予频率为11mHz,强度为500 Gs的磁刺激,每次15 min,1次/d;假刺激组放入磁刺激仪通电,但不给予刺激;假手术组自然饲养.分组后第7天、第14天及第21天应用18分制神经功能障碍评分方法对各组大鼠进行神经功能评定,同时应用免疫组化染色检测各组大鼠海马区nestin、Brdu阳性细胞数. 结果分组后第14天、21天,11 mHz刺激组神经功能障碍评分明显高于假刺激组与假手术组,差异有统计学意义(P＜0.05).分组后第7天、第14天及第21天,11mHz组nestin阳性细胞数量均明显多于假刺激组与假手术组,差异有统计学意义(P＜0.05).分组后第7天、第14天及第21天,11mHz组Brdu阳性细胞数量均明显多于假手术组,差异有统计学意义(P＜0.05);但11mHz组与假刺激组Brdu阳性细胞数差异无统计学意义(P＞0.05). 结论脑损伤本身可以一定程度地刺激海马区nestin的表达,而11mHz超低频经颅磁刺激可以促进这种表达,进而促进脑损伤后神经修复;但11 mHz超低频经颅磁刺激对对细胞的增殖无明显影响.
목적 관찰11 mHz초저빈경로자자격(ILF-TMS)대대서국부뇌결혈재관주손상모형해마구소단백(nestin)、5-탈양뇨밀정핵감(Brdu)표체적영향. 방법 대서안수궤수자표법분위3조,분별위11 mHz조、가자격조화가수술조,매조24지.11 mHz조、가자격조삼조Longa등적선전법건립대서우측뇌결혈재관주손상모형.가수술조마취대서후,둔성분리각혈관단불결찰,역불진선.11mHz조급여빈솔위11mHz,강도위500 Gs적자자격,매차15 min,1차/d;가자격조방입자자격의통전,단불급여자격;가수술조자연사양.분조후제7천、제14천급제21천응용18분제신경공능장애평분방법대각조대서진행신경공능평정,동시응용면역조화염색검측각조대서해마구nestin、Brdu양성세포수. 결과 분조후제14천、21천,11 mHz자격조신경공능장애평분명현고우가자격조여가수술조,차이유통계학의의(P＜0.05).분조후제7천、제14천급제21천,11mHz조nestin양성세포수량균명현다우가자격조여가수술조,차이유통계학의의(P＜0.05).분조후제7천、제14천급제21천,11mHz조Brdu양성세포수량균명현다우가수술조,차이유통계학의의(P＜0.05);단11mHz조여가자격조Brdu양성세포수차이무통계학의의(P＞0.05). 결론 뇌손상본신가이일정정도지자격해마구nestin적표체,이11mHz초저빈경로자자격가이촉진저충표체,진이촉진뇌손상후신경수복;단11 mHz초저빈경로자자격대대세포적증식무명현영향.
Objective To investigate the effect of 11 mHz ultra-low frequency transcranial magnetic stimulation (ILF-TMS) on neural function recovery and expressions ofnestin and Brdu in the hippocampus of cerebral ischemia and reperfusion rats.Methods Seventy-two rats were used in our study and divided into 11 mHz ILF-TMS group,sham-stimulated group and sham-operated group (n=24);The filament method was used to establish the focal cerebral ischemia and reperfusion (I-R) rat models in the first two groups.Rats in the sham-operated group only performed blood vessel separation without ligation; rats in the 11 mHz ILF-TMS group were given magnetic stimulation at 11 rnHz and 500 Gs for 15 min once daily; rats in the sham-stimulated group were only inputted magnetic stimulator.Seven,14 and 21 d after the group division,the recovery of neurological function was evaluated by 18-point nerve dysfunction scoring method,and immunohistochemistry was used to detect the nestin and Brdu positive cells in the hippocampus of rats in each group.Results The neurological deficit scores in the 11 mHz ILF-TMS group were significantly higher than those in the sham-stimulated group and sham-operated group 14 and 21 d after group after dividing (P＜0.05).As compared with sham-stimulated group and sham-operated group,1 mHz ILF-TMS group had increased number of nestin positive cells,with significant difference on the 7th,14th and 21st d; there was no statistical significance in the number of Brdu positive cells between sham-stimulated group and 1 mHz ILF-TMS group (P＜0.05).Conclusions Brain injury can stimulate the expression of nestin and Brdu in a certain degree.Although 11 mHz ultra-low frequency transcranial magnetic stimulation can improve the expression of nestin more effectively,the function of promoting cell proliferation of Brdu-positive cells is not observed.