CAJ | 학술논문

目的探讨性别因素对小鼠大脑皮层扩散性抑制(SD)波的影响情况. 方法成年C57小鼠按照双盲随机方法被分为4组:雄性KCl诱导组,雌性KCl诱导组,雄性MCAO诱导组和雌性MCAO诱导组,每组各8只.利用氯化钾(KCl)和大脑中动脉梗死(MCAO)模型诱导SD波的发作,电生理监测2h内各组小鼠SD波的发作情况. 结果 MCAO诱导组与KCl诱导组相比,SD波波形不同,波幅增大,时程延长;雄性和雌性KC1诱导组的SD波波幅、时程、发作次数及首次发作时间差异无统计学意义(P＞0.05);雄性和雌性MCAO诱导组的SD波波幅及时程差异无统计学意义(P＞0.05),但2组的SD波发作次数及首次发作时间差异有统计学意义(P＜0.05). 结论性别因素对KC1诱导的SD波发作无显著影响,但影响MCAO诱导的SD波发作;该差异可能与雌激素主要通过谷氨酸途径而非K+代谢途径影响SD波的发作有关.
목적 탐토성별인소대소서대뇌피층확산성억제(SD)파적영향정황. 방법 성년C57소서안조쌍맹수궤방법피분위4조:웅성KCl유도조,자성KCl유도조,웅성MCAO유도조화자성MCAO유도조,매조각8지.이용록화갑(KCl)화대뇌중동맥경사(MCAO)모형유도SD파적발작,전생리감측2h내각조소서SD파적발작정황. 결과 MCAO유도조여KCl유도조상비,SD파파형불동,파폭증대,시정연장;웅성화자성KC1유도조적SD파파폭、시정、발작차수급수차발작시간차이무통계학의의(P＞0.05);웅성화자성MCAO유도조적SD파파폭급시정차이무통계학의의(P＞0.05),단2조적SD파발작차수급수차발작시간차이유통계학의의(P＜0.05). 결론 성별인소대KC1유도적SD파발작무현저영향,단영향MCAO유도적SD파발작;해차이가능여자격소주요통과곡안산도경이비K+대사도경영향SD파적발작유관.
Objective To observe the influence of gender in cortical spreading depression (SD) wave in mice.Methods Potassium chloride (KCl) or middle cerebral artery occlusion (MCAO) models were used to induce SD.Adult C57 mice were randomly divided into four groups:male and female KCl-induced groups,male and female MCAO-induced groups (n=8).SD waves were monitored for 2 h by electrophysiological method.Results SD waves showed lower amplitude and longer duration time in the MCAO-induced group as compared with those in the KCl-induced group.There were no obvious differences in SD amplitude,duration time,frequency and onset time of first SD between male and female KCl-induced groups (P＞0.05).There were no differences in SD amplitude and duration time between male and female MCAO-induced groups (P＞0.05),however,SD frequency and onset time of the first SD were signficantly different between male and female MCAO-induced group (P＜0.05).Conclusion Mice gender has an effect on MCAO-induced SD,but no obvious effect on KCl-induced SD; the possible explanation for the difference is that estrogens modifying susceptibility to SD is mainly through their effect on glutamate but not on K+ metabolic pathways.