CAJ | 학술논문

目的观察非酒精性脂肪性肝病(NAFLD)患者肝组织固醇调节元件结合蛋白(SREBP1c)的表达变化,探讨其在NAFLD病理变化形成中的作用.方法研究对象为NAFLD患者20例,20例年龄性别匹配的同期住院行肝活检无活动肝病组织学证据且肝脂肪变＜5％的慢性HBV携带者(ASC)设为对照组.免疫组织化学染色检测两组患者肝组织SREBP1c的表达,并比较血脂(TG、CHO)、空腹血糖(FBG)、空腹胰岛素(FINS)和血清肝脏酶谱(AST、ALT、GGT)等的组间差异.结果与对照组患者比较,NAFLD组肝组织呈不同程度的弥漫性肝细胞脂肪变性,肝组织SREBP1c表达明显升高(P＜0.05),与此一致,血清ALT、AST、TG、CHO、FBG、FINS水平升高(P均＜0.05).结论 NAFLD患者肝组织SREBP1 c表达增高,在人NAFLD脂肪变形成过程中起重要作用.
목적 관찰비주정성지방성간병(NAFLD)환자간조직고순조절원건결합단백(SREBP1c)적표체변화,탐토기재NAFLD병리변화형성중적작용.방법 연구대상위NAFLD환자20례,20례년령성별필배적동기주원행간활검무활동간병조직학증거차간지방변＜5％적만성HBV휴대자(ASC)설위대조조.면역조직화학염색검측량조환자간조직SREBP1c적표체,병비교혈지(TG、CHO)、공복혈당(FBG)、공복이도소(FINS)화혈청간장매보(AST、ALT、GGT)등적조간차이.결과 여대조조환자비교,NAFLD조간조직정불동정도적미만성간세포지방변성,간조직SREBP1c표체명현승고(P＜0.05),여차일치,혈청ALT、AST、TG、CHO、FBG、FINS수평승고(P균＜0.05).결론 NAFLD환자간조직SREBP1 c표체증고,재인NAFLD지방변형성과정중기중요작용.
Objective To investigate the hepatic expression of sterol regulatory element binding protein (SREBP)-1c in patients with non-alcoholic fatty liver disease (NAFLD) and its significance.Methods Twenty patients with NAFLD were included,and 20 age/sex matched asymptomatic HBV carriers (ASC) without steatosis evidenced by liver biopsy were used as control in the same time scale.Hepatic SREBP1c was detected using SP method of immunohistochemistry.An automatic biochemical analyzer (Hitachi 7060) was used to measure serum levels of lipids (TG,CHO),fasting blood glucose (FBG) and liver enzymes (AST,A LT,and GGT),and electrochemiluminescence was used to determine fasting insulin (FINS).Results Compared with the control group,NAFLD patients showed diffusely hepatic steatosis and a significantly higher expression of hepatic SREBP1c (P ＜ 0.05).Consistently,serum levels of ALT,AST,TG,CHO,FSG and FINS all increased than those of control group (P ＜ 0.05).Conclusions Hepatic expression of SREBP1c increased significantly in patients with NAFLD,and this over expression may be involve in fatty degeneration of the livers in human.