CAJ | 학술논문

目的探讨汉族儿童呼吸道合胞病毒(respiratory syncytial virus,RSV)毛细支气管炎肺表面活性蛋白D(surfactant protein D,SP-D)基因多态性与甲强龙治疗疗效的关系.方法采用MGB测序法检测150例RSV毛细支气管炎和150例健康对照组SP-D-11位点、160位点基因多态性,并进行基因型、等位基因型频率分析,分析基因多态性和毛细支气管炎发病的关系,采用(x2)检验.150例患儿在常规治疗基础上加用甲强龙静脉滴注,连用3d.分析SP-D基因多态性对甲强龙治疗疗效的影响,采用方差(F)分析.结果病例组SP-D Met11Thr位点TT、CT、CC三种基因型频率分别为7.3％、44.0％、48.7％,T、C等位基因频率分别为29.3％、70.7％.三种基因型及等位基因频率与对照组比较差异有统计学意义(x2 =6.751、5.823,P＜0.05).SP-D Ala160Thr位点AA、GA、GG三种基因型频率分别为4.0％、29.3％、66.7％,A、G等位基因频率分别为18.7％、71.3％,两组基因型及等位基因频率与对照组比较差异无统计学意义(x2 =0.182、0.181,P＞0.05).不同基因型在平均住院天数、临床症状改善差异均无统计学意义(P＞0.05).结论杭州地区汉族儿童存在SP-D基因多态性,SP-DMet1 1Thr位点与RSV毛细支气管炎疾病易感性存在关联,SP-D Met11Thr C等位基因可能易感基因;而SP-D Ala160Thr位点与RSV毛细支气管炎疾病易感性无关联.SP-DMet1 1Thr、SP-D Ala160Thr的多态性并不影响甲强龙治疗的疗效.
목적 탐토한족인동호흡도합포병독(respiratory syncytial virus,RSV)모세지기관염폐표면활성단백D(surfactant protein D,SP-D)기인다태성여갑강룡치료료효적관계.방법 채용MGB측서법검측150례RSV모세지기관염화150례건강대조조SP-D-11위점、160위점기인다태성,병진행기인형、등위기인형빈솔분석,분석기인다태성화모세지기관염발병적관계,채용(x2)검험.150례환인재상규치료기출상가용갑강룡정맥적주,련용3d.분석SP-D기인다태성대갑강룡치료료효적영향,채용방차(F)분석.결과 병례조SP-D Met11Thr위점TT、CT、CC삼충기인형빈솔분별위7.3％、44.0％、48.7％,T、C등위기인빈솔분별위29.3％、70.7％.삼충기인형급등위기인빈솔여대조조비교차이유통계학의의(x2 =6.751、5.823,P＜0.05).SP-D Ala160Thr위점AA、GA、GG삼충기인형빈솔분별위4.0％、29.3％、66.7％,A、G등위기인빈솔분별위18.7％、71.3％,량조기인형급등위기인빈솔여대조조비교차이무통계학의의(x2 =0.182、0.181,P＞0.05).불동기인형재평균주원천수、림상증상개선차이균무통계학의의(P＞0.05).결론 항주지구한족인동존재SP-D기인다태성,SP-DMet1 1Thr위점여RSV모세지기관염질병역감성존재관련,SP-D Met11Thr C등위기인가능역감기인;이SP-D Ala160Thr위점여RSV모세지기관염질병역감성무관련.SP-DMet1 1Thr、SP-D Ala160Thr적다태성병불영향갑강룡치료적료효.
Objective To investigate the influence of genetic polymorphisms within surfactant protein(SP-D) gene on the development of respiratory syncytial virus(RSV) bronchiolitis and the clinical outcomes of the individual response to methylprednisolone therapy in Han ethnicity in Hangzhou.Methods Genotyping of SP-D polymorphisms was performed by polymerase chain reaction and TaqMan MGB probe in 150 cases of RSV bronchitis (case group) and 150 healthy children (control group),and the genotype frequencies and allele frequencies were analyzed simultaneously.Each group' s genotype and allele frequencies were compared with x2 test; 150 cases of bronchiolitis received conventional therapy,using methylprednisolone injection treatment.Analysis on the effect of SP-D gene polymorphism on the methylprednisolone therapy was performed with F test.Results The frequencies of genotypes TT,TC and CC of SP-D Met1 1Thr were 7.3％,44.0％ and 48.7％,respectively,and the allele frequencies of T and C were 29.3％ and 70.7％,respectively.SP-D Met11Thr displayed significant association with RSV as compared with control group (x2 =6.751,5.823,P ＜ 0.05).The frequencies of AA,GA,GG of SP-D Alal60Thr were 4.0％,29.3％ and 66.7 ％,respectively,and the allele frequencies of A and G were 18.7％ and 71.3％.SP-D Ala160Thr displayed no significant association with RSV bronchiolitis as compared with control group (x2 =0.182,0.181,P ＞ 0.05).Different genotypes in the average hospitalization days,clinicalsymptoms had no statistically significant difference (P ＞ 0.05).Conclusion Both SP-D MetllThr and SP-D Ala160Thr gene polymorphisms were present in Han children in Hangzhou area,Polymorphisms of SP-D Metl1Thr displayed significant association with RSV bronchiolitis as compared with control group,SP-D Met11Thr C allele may be a susceptibility gene.Polymorphisms of SP-D Ala160Thr did not correlate with RSV as compared with control group.SP-D variation had no effect on therapeutic response to methylprednisolone in patients with RSV bronchiolitis.