中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
12期
2664-2666
,共3页
杨清滔%谷江%张永春%朱致晖%杨永安%王楠%祝庆亮%姚茂良
楊清滔%穀江%張永春%硃緻暉%楊永安%王楠%祝慶亮%姚茂良
양청도%곡강%장영춘%주치휘%양영안%왕남%축경량%요무량
激素非依赖性前列腺癌%京尼平%解耦联蛋白2%抑制
激素非依賴性前列腺癌%京尼平%解耦聯蛋白2%抑製
격소비의뢰성전렬선암%경니평%해우련단백2%억제
Androgen independent prostate cancer cells%Genipin%Uncoupling protein 2%Inhibition
目的 从能量生成角度研究京尼平(Genipin)对激素非依赖性前列腺癌(AIPC)的抑制作用是否与线粒体解耦联蛋白2(UCP2)被阻断有关,并探讨其临床意义.方法 以低、中、高(40、80、120 μmol/L)剂量Genipin干预PC-3细胞培养基48 h,噻唑蓝(MTT)和流式细胞仪检测细胞增殖及凋亡,实时荧光定量聚合酶链反应(Real-time PCR)、Western blot检测UCP2表达,荧光探针检测细胞内Ca2含量,分光光度计检测线粒体膜电势(△ψm)、膜通透性转换孔(mPTP)变化及三磷酸腺苷(ATP)含量.结果 Genipin能显著抑制PC-3细胞增殖,其中对照组及低、中、高剂量组细胞凋亡率分别为(7.60±1.48)％、(9.38±1.01)％、(20.77±1.14)％、(23.48±1.99)％,Genipin可降低UCP2表达,此时细胞内Ca2含量明显增加,且mPTP开放程度增加及△ψm减小,ATP生成明显减少,其中对照组及低、中、高剂量组ATP含量分别为(0.120 ±0.015)、(0.110 ±0.013)、(0.080 ±0.007)、(0.050 ±0.008) mmol/L,以上改变均与Genipin浓度呈正相关,且各组间比较差异均有统计学意义(P＜0.05).结论 Genipin能明显抑制AIPC细胞的增殖,该抑制作用可能与其阻断UCP2,导致线粒体功能异常,ATP生成减少有关.
目的 從能量生成角度研究京尼平(Genipin)對激素非依賴性前列腺癌(AIPC)的抑製作用是否與線粒體解耦聯蛋白2(UCP2)被阻斷有關,併探討其臨床意義.方法 以低、中、高(40、80、120 μmol/L)劑量Genipin榦預PC-3細胞培養基48 h,噻唑藍(MTT)和流式細胞儀檢測細胞增殖及凋亡,實時熒光定量聚閤酶鏈反應(Real-time PCR)、Western blot檢測UCP2錶達,熒光探針檢測細胞內Ca2含量,分光光度計檢測線粒體膜電勢(△ψm)、膜通透性轉換孔(mPTP)變化及三燐痠腺苷(ATP)含量.結果 Genipin能顯著抑製PC-3細胞增殖,其中對照組及低、中、高劑量組細胞凋亡率分彆為(7.60±1.48)％、(9.38±1.01)％、(20.77±1.14)％、(23.48±1.99)％,Genipin可降低UCP2錶達,此時細胞內Ca2含量明顯增加,且mPTP開放程度增加及△ψm減小,ATP生成明顯減少,其中對照組及低、中、高劑量組ATP含量分彆為(0.120 ±0.015)、(0.110 ±0.013)、(0.080 ±0.007)、(0.050 ±0.008) mmol/L,以上改變均與Genipin濃度呈正相關,且各組間比較差異均有統計學意義(P＜0.05).結論 Genipin能明顯抑製AIPC細胞的增殖,該抑製作用可能與其阻斷UCP2,導緻線粒體功能異常,ATP生成減少有關.
목적 종능량생성각도연구경니평(Genipin)대격소비의뢰성전렬선암(AIPC)적억제작용시부여선립체해우련단백2(UCP2)피조단유관,병탐토기림상의의.방법 이저、중、고(40、80、120 μmol/L)제량Genipin간예PC-3세포배양기48 h,새서람(MTT)화류식세포의검측세포증식급조망,실시형광정량취합매련반응(Real-time PCR)、Western blot검측UCP2표체,형광탐침검측세포내Ca2함량,분광광도계검측선립체막전세(△ψm)、막통투성전환공(mPTP)변화급삼린산선감(ATP)함량.결과 Genipin능현저억제PC-3세포증식,기중대조조급저、중、고제량조세포조망솔분별위(7.60±1.48)％、(9.38±1.01)％、(20.77±1.14)％、(23.48±1.99)％,Genipin가강저UCP2표체,차시세포내Ca2함량명현증가,차mPTP개방정도증가급△ψm감소,ATP생성명현감소,기중대조조급저、중、고제량조ATP함량분별위(0.120 ±0.015)、(0.110 ±0.013)、(0.080 ±0.007)、(0.050 ±0.008) mmol/L,이상개변균여Genipin농도정정상관,차각조간비교차이균유통계학의의(P＜0.05).결론 Genipin능명현억제AIPC세포적증식,해억제작용가능여기조단UCP2,도치선립체공능이상,ATP생성감소유관.
Objective To investigate whether uncoupling protein 2 (UCP2) blocked was participated in genipin inhibiting proliferation of androgen independent prostate cancer cells from the perspective of energy,and to explore its clinical significance.Methods PC-3 cells were cultured in vitro,three concentrations of genipin solutions were added to the culture medium.The differences in proliferation,apoptosis,expressions of UCP2,levels of Ca2+,mitochondrion membrane potential (△ψm),membrane permeability transition pore (mPTP) and adenosine triphosphate (ATP) between before and after treated with various concentrations of genipin were measured by methyl thiazolyl tetrazolium (MTT) assay,flow cytometry assay,Real-time polymerase chain reaction (PCR) assay,Western blotting assay,spectrophotometer assay respectively.Results Genipin significantly suppressed the growth of PC-3 cells.After treatment with different concentration of genipin for 48 h,the apoptosis rate of PC-3 cells was (7.60 ± 1.48) ％,(9.38 ±1.01) ％,(20.77 ± 1.14) ％ and (23.48 ± 1.99) ％ respectively,expression of UCP2 was significantly reduced,contents of Ca2+ and mPTP open degree were significantly increased,level of △ψm and contents of ATP were significantly reduced simultaneously,the contents of ATP was (0.120 ± 0.015),(0.110 ± 0.013),(0.080 ± 0.007) and (0.050 ± 0.008) mmol/L respectively.The above changes with genipin as a dose-dependent manner,and the differences were statistically significant (P ＜ 0.05).Conclusion Genipin significantly inhibited the growth of androgen independent prostate cancer cells,of which may occur through down-regulating the expression of UCP2,resulting in mitochondrial dysfunction and ATP production decreased.
