CAJ | 학술논문

目的观察阿托伐他汀对动脉粥样硬化及脂蛋白受体表达的影响.方法采用显微外科缝合法造成兔颈动脉狭窄,并联合高胆同醇饮食建立兔颈动脉粥样硬化模型,应用阿托伐他汀进行干预,应用苏木素-伊红(HE)、Masson染色切片观察不同时间颈动脉壁的病理改变,以内膜/中膜厚度比值(I/M)及内膜/中膜面积比值(SI/SM)评价动脉粥样硬化程度,免疫组织化学法检测颈动脉内膜脂蛋白受体LOX-1的表达.结果采用显微缝合联合高脂饮食法成功诱导颈动脉内膜增厚及动脉粥样硬化斑块形成,6周他汀组I/M及SI/SM (0.49 ±0.51、0.11 ±0.09)均明显低于对照组(0.88±0.39、0.28±0.13,P＜0.05);12周他汀组I/M及SL/SM(0.68±0.43、0.61±0.22)均明显低于对照组(2.64 ±0.62、1.88 ±0.47,P＜0.05);颈动脉内膜脂蛋白受体LOX-1表达6、12周(0.04±0.02、0.72±0.18)均低于同期对照组(2.12±0.67、5.06±0.78,P＜0.05).结论应用显微缝合联合高脂饮食法成功建立兔颈动脉粥样硬化模型,阿托伐他汀可抑制或延缓动脉粥样硬化斑块形成及脂蛋白受体LOX-1表达.
목적 관찰아탁벌타정대동맥죽양경화급지단백수체표체적영향.방법 채용현미외과봉합법조성토경동맥협착,병연합고담동순음식건립토경동맥죽양경화모형,응용아탁벌타정진행간예,응용소목소-이홍(HE)、Masson염색절편관찰불동시간경동맥벽적병리개변,이내막/중막후도비치(I/M)급내막/중막면적비치(SI/SM)평개동맥죽양경화정도,면역조직화학법검측경동맥내막지단백수체LOX-1적표체.결과 채용현미봉합연합고지음식법성공유도경동맥내막증후급동맥죽양경화반괴형성,6주타정조I/M급SI/SM (0.49 ±0.51、0.11 ±0.09)균명현저우대조조(0.88±0.39、0.28±0.13,P＜0.05);12주타정조I/M급SL/SM(0.68±0.43、0.61±0.22)균명현저우대조조(2.64 ±0.62、1.88 ±0.47,P＜0.05);경동맥내막지단백수체LOX-1표체6、12주(0.04±0.02、0.72±0.18)균저우동기대조조(2.12±0.67、5.06±0.78,P＜0.05).결론 응용현미봉합연합고지음식법성공건립토경동맥죽양경화모형,아탁벌타정가억제혹연완동맥죽양경화반괴형성급지단백수체LOX-1표체.
Objective To evaluate the effect of atorvastatin on the formation of artherosclerosis and expression of Lipoprotein receptor LOX-1.Methods Animal models of carotid artery artherosclerosis were successively established by microsurgical suture performed on carotid artery in rabbits in combination with high fat diet,and treated with atorvastatin orally.In the contral group,no atorvastatin was given.Rabbits were sacrificed in batch at 6th and 12th week with gas embolism.The pathological changes of the carotid were observed by hematoxylin and eosin (HE) staining and HE/Masson staining.The artherosclerosis was evaluated by ratio of intima (Ⅰ) thickness to tunica media (M) thickness,and ration of intima (SI) areas to tunica media (SM) areas.Immunohistochemical staining was used to detect the expression of LOX-1.Inage-ProPlus image analysis system was used to analyze the positive area ratio of LOX-1.Results The carotid atherosclerosis model was successfully established by microsurgical suture in combination with high fat diet.The I/M ratio and SI/SM ratio were significantly reduced at 6th week (0.49 ± 0.51 and 0.11 ±0.09) and at 12 week (0.68 ±0.43 and 0.61 ±0.22) in the atorvastatin group as compared with those in the control group (0.88 ±0.39 and 0.28 ±0.13 at 6th week,and 2.64 ±0.62 and 1.88 ±0.47 at 12th week,P＜0.05 for all).The expression of LOX-1 at 6th week (0.04 ±0.02) and at 12th week (0.72 ±0.18) was lower in the atorvastatin groups than in the control (2.12 ± 0.67 at 6th week,and 5.06 ± 0.78 at 12th week,P ＜ 0.05).Conclusion Carotid artery atherosclerosis model was successfully established by microsurgical suture in combination with high fat diet.Atorvastatins could inhibit or delay the formation of atherosclerosis and suppress the expression of LOX-1 in rabbit carotid artery atherosclerosis models.