中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
12期
2735-2737
,共3页
刘郑和%申东方%王明太%陈宗然
劉鄭和%申東方%王明太%陳宗然
류정화%신동방%왕명태%진종연
羟乙基淀粉%肝脏缺血%再灌注损伤%氧自由基
羥乙基澱粉%肝髒缺血%再灌註損傷%氧自由基
간을기정분%간장결혈%재관주손상%양자유기
Hydroxyethyl starch%Liver ischemia%Reperfusion injury%Oxygen free radicals
目的 探讨羟乙基淀粉130/0.4(HES130/0.4)对大鼠肝脏缺血/再灌注损伤的保护作用及其机制.方法 40只雌性SD大鼠随机分为4组:假手术组(Sham组)、缺血/再灌注组(IR组)、缺血/再灌注+7.5％高渗盐水组(IR+ HS组)、缺血/再灌注+羟乙基淀粉组(IR+ HES组),每组10只.IR+ HES和IR+ HS组在肝缺血60 min后分别静脉持续泵入HES130/0.4、7.5％ HS(13 ml/kg),再灌注120 min.收集血液进行血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)测定,取部分肝组织行超氧化物歧化酶(SOD)、丙二醛(MDA)测定及病理学检查.结果 与Sham组比较,血清ALT、AST、肝组织MDA等指标在IR组[(481.64±86.32) IU/L、(793.16±86.51) IU/L、(6.45±0.63) nmol/(mg·蛋白)]、IR+HS组[(461.44±76.35) IU/L、(784.58±81.05) IU/L、(6.25±0.69) nmol/(mg·蛋白)]及IR+ HES组[(230.23±51.85) IU/L、(330.14±23.52) IU/L、(5.04±0.62) nmol/(mg·蛋白)]均升高(P＜ 0.05);SOD活性[(129.35±17.05)、(133.64±16.60)、(160.25±28.80) U/(mg·蛋白)]降低(P＜0.05);肝组织病理学评分升高(P＜0.05).结论 给予羟乙基淀粉130/0.4(HES130/0.4)对大鼠肝脏缺血/再灌注损伤具有保护作用,其机制可能与加速氧自由基清除,降低氧自由基介导的脂质过氧化反应有关.
目的 探討羥乙基澱粉130/0.4(HES130/0.4)對大鼠肝髒缺血/再灌註損傷的保護作用及其機製.方法 40隻雌性SD大鼠隨機分為4組:假手術組(Sham組)、缺血/再灌註組(IR組)、缺血/再灌註+7.5％高滲鹽水組(IR+ HS組)、缺血/再灌註+羥乙基澱粉組(IR+ HES組),每組10隻.IR+ HES和IR+ HS組在肝缺血60 min後分彆靜脈持續泵入HES130/0.4、7.5％ HS(13 ml/kg),再灌註120 min.收集血液進行血清丙氨痠轉氨酶(ALT)、天鼕氨痠轉氨酶(AST)測定,取部分肝組織行超氧化物歧化酶(SOD)、丙二醛(MDA)測定及病理學檢查.結果 與Sham組比較,血清ALT、AST、肝組織MDA等指標在IR組[(481.64±86.32) IU/L、(793.16±86.51) IU/L、(6.45±0.63) nmol/(mg·蛋白)]、IR+HS組[(461.44±76.35) IU/L、(784.58±81.05) IU/L、(6.25±0.69) nmol/(mg·蛋白)]及IR+ HES組[(230.23±51.85) IU/L、(330.14±23.52) IU/L、(5.04±0.62) nmol/(mg·蛋白)]均升高(P＜ 0.05);SOD活性[(129.35±17.05)、(133.64±16.60)、(160.25±28.80) U/(mg·蛋白)]降低(P＜0.05);肝組織病理學評分升高(P＜0.05).結論 給予羥乙基澱粉130/0.4(HES130/0.4)對大鼠肝髒缺血/再灌註損傷具有保護作用,其機製可能與加速氧自由基清除,降低氧自由基介導的脂質過氧化反應有關.
목적 탐토간을기정분130/0.4(HES130/0.4)대대서간장결혈/재관주손상적보호작용급기궤제.방법 40지자성SD대서수궤분위4조:가수술조(Sham조)、결혈/재관주조(IR조)、결혈/재관주+7.5％고삼염수조(IR+ HS조)、결혈/재관주+간을기정분조(IR+ HES조),매조10지.IR+ HES화IR+ HS조재간결혈60 min후분별정맥지속빙입HES130/0.4、7.5％ HS(13 ml/kg),재관주120 min.수집혈액진행혈청병안산전안매(ALT)、천동안산전안매(AST)측정,취부분간조직행초양화물기화매(SOD)、병이철(MDA)측정급병이학검사.결과 여Sham조비교,혈청ALT、AST、간조직MDA등지표재IR조[(481.64±86.32) IU/L、(793.16±86.51) IU/L、(6.45±0.63) nmol/(mg·단백)]、IR+HS조[(461.44±76.35) IU/L、(784.58±81.05) IU/L、(6.25±0.69) nmol/(mg·단백)]급IR+ HES조[(230.23±51.85) IU/L、(330.14±23.52) IU/L、(5.04±0.62) nmol/(mg·단백)]균승고(P＜ 0.05);SOD활성[(129.35±17.05)、(133.64±16.60)、(160.25±28.80) U/(mg·단백)]강저(P＜0.05);간조직병이학평분승고(P＜0.05).결론 급여간을기정분130/0.4(HES130/0.4)대대서간장결혈/재관주손상구유보호작용,기궤제가능여가속양자유기청제,강저양자유기개도적지질과양화반응유관.
Objective To observe the protective effect and mechanism of hydroxyethyl starch 130/ 0.4 (HES130/0.4) against early injury of hepatic ischemia/reperfusion in rats.Methods Forty female SD rats were randomly divided into 4 groups:sham operation group (Sham group),ischemia/reperfusion group (IR group),ischemia/reperfusion + 7.5％ hypertonic saline group (IR + HS group),and ischemia/reperfusion + hydroxyethyl starch group (IR + HES group),n =10 each.The rats in IR + HES group and IR + HS group were subjected to hepatic ischemia for 60 min,followed by intravenous infusion of HES 130/0.4 and 7.5％ HS (13 ml/kg),and finally 120 min of reperfusion.Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined.Superoxide dismutase (SOD),malondialdehyde (MDA) and histopathological changes in the liver tissue were examined.Results As compared with Sham group,serum ALT and AST,and liver MDA in the IR group [(481.64 ±86.32),(793.16 ± 86.51) IU/L,and (6.45 ±0.63) nmol/(mg·prot)],IR + HS group [(461.44 ±76.35),(784.58 ± 81.05) IU/L,and (6.25 ±0.69) nmol/(mg·prot)] and IR + HES group [(230.23 ±51.85),(330.14 ± 23.52) IU/L,and (5.04 ± 0.62) nmol/(mg· prot)] were significantly increased (P ＜ 0.05) ; SOD activity [(129.35 ± 17.05),(133.64 ± 16.60) and (160.25 ± 28.80) U/(mg· prot)] reduced (P＜0.05) ; liver histopathology scores were increased (P＜0.05).Conclusion HES 130/0.4 has protective effects on hepatic ischemia/reperfusion in rats,which may be related to accelerate the elimination of oxygen free radicals,and reduce oxygen free radical-mediated lipid peroxidation.
