CAJ | 학술논문

目的观察XAV939对胃癌细胞株MKN-45增殖及Wnt/β-连环蛋白(Wnt/β-catenin)信号通路的作用.方法检测XAV939对胃癌细胞株MKN-45细胞的增殖抑制作用,逆转录-聚合酶链反应(RT-PCR)法检测β-catenin和细胞周期蛋白D1(Cyclin D1) mRNA表达,Western blot法检测Axin 1、β-catenin、磷酸化β-catenin(p-β-catenin)和Cyclin D1的表达.结果不同浓度的XAV939对胃癌细胞株MKN-45细胞增殖的抑制率分别是22.5％、39.1％、52.5％、62.1％(P＜0.05);不同浓度的XAV-939处理组的β-catenin、Cyclin D1 mRNA表达下降(分别为0.727±0.031、0.467±0.051、0.353±0.035、0.293±0.031,P ＜0.05;0.690 ±0.161、0.487±0.231、0.360±0.257、0.216±0.197,P ＜0.05),β-catenin、Cyclin D1蛋白表达下降而Axin 1和p-β-catenin蛋白表达上升.结论 XAV-939可抑制以胃癌细胞株MKN-45的增殖,机制可能是通过抑制Wnt/β-catenin通路.
목적 관찰XAV939대위암세포주MKN-45증식급Wnt/β-련배단백(Wnt/β-catenin)신호통로적작용.방법 검측XAV939대위암세포주MKN-45세포적증식억제작용,역전록-취합매련반응(RT-PCR)법검측β-catenin화세포주기단백D1(Cyclin D1) mRNA표체,Western blot법검측Axin 1、β-catenin、린산화β-catenin(p-β-catenin)화Cyclin D1적표체.결과 불동농도적XAV939대위암세포주MKN-45세포증식적억제솔분별시22.5％、39.1％、52.5％、62.1％(P＜0.05);불동농도적XAV-939처리조적β-catenin、Cyclin D1 mRNA표체하강(분별위0.727±0.031、0.467±0.051、0.353±0.035、0.293±0.031,P ＜0.05;0.690 ±0.161、0.487±0.231、0.360±0.257、0.216±0.197,P ＜0.05),β-catenin、Cyclin D1단백표체하강이Axin 1화p-β-catenin단백표체상승.결론 XAV-939가억제이위암세포주MKN-45적증식,궤제가능시통과억제Wnt/β-catenin통로.
Objective To investigate the effects of XAV939 on proliferation and and Wnt/β-catenin signaling pathway of MKN-45 cells in vitro.Methods MKN-45 cells were cultured with different concentrations of XAV939.The inhibition of cell proliferation was detected by methyl thiazol tetrazolium (MTT).The expression levels of β-catenin and cell cycle protein 1 (Cyclin D1) mRNA were detected by reverse transcription-polymerase chain reaction (RT-PCR),and the protein expression of Axin 1,β-catenin,p-β-catenin and Cyclin D1 by Western blotting.Results Inhibitory rate of MKN-45 cells treated with XAV939 for 48 h was 22.5％,39.1％,52.5％,and 62.1％ respectively (P ＜0.05).Different concentrations of XAV939 could down-regulate β-catenin and Cyclin D1 mRNA level (0.727 ±0.031,0.467 ±0.051,0.353 ±0.035,0.293 ±0.031,P＜0.05; 0.690±0.161,0.487 ±0.231,0.360 ±0.257,0.2 1 6 ± 0.197,P ＜ 0.05),down-regulate β-catenin and Cyclin D1 protein levels,but significantly up-regulate Axin 1 and p-β-catenin expression levels.Conclusion XAV939 can inhibite proliferation of MKN-45 cells,which may be associated with down-regulation of Wnt/β-catenin signaling pathway.