中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
12期
2754-2755
,共2页
免疫功能%射频%保脾%脾切除
免疫功能%射頻%保脾%脾切除
면역공능%사빈%보비%비절제
Immune function%Radiofrequency ablation%Spleen preserving%Splenectomy
目的 观察射频保脾及脾切除术后机体免疫功能的改变.方法 使用随机分组的方法将实验用长白猪分为正常对照组(10头)、射频保脾组(10头)、脾切除组(10头).分别于术后1、4、12、24周采静脉血检测机体免疫功能指标:使用酶联免疫吸附试验(ELISA)法检测血清IgM、IgG、C3、C4水平;使用流式细胞仪检测外周血T淋巴细胞CD4+、CD8+比例.结果 在体液免疫方面,IgM水平在射频保脾术后无明显变化,而脾切除组术后IgM水平显著降低;IgG、C3、C4血浆浓度以及CD4+、CD8+T淋巴细胞比例在术后监测中,各组差异均无统计学意义(P＞0.05).结论 射频保脾对机体短期内体液免疫功能无明显损害,脾切除术后对机体短期体液免疫功能有损害;而无论是射频保脾还是脾脏切除对机体远期体液免疫功能及细胞免疫功能可能无明显影响.
目的 觀察射頻保脾及脾切除術後機體免疫功能的改變.方法 使用隨機分組的方法將實驗用長白豬分為正常對照組(10頭)、射頻保脾組(10頭)、脾切除組(10頭).分彆于術後1、4、12、24週採靜脈血檢測機體免疫功能指標:使用酶聯免疫吸附試驗(ELISA)法檢測血清IgM、IgG、C3、C4水平;使用流式細胞儀檢測外週血T淋巴細胞CD4+、CD8+比例.結果 在體液免疫方麵,IgM水平在射頻保脾術後無明顯變化,而脾切除組術後IgM水平顯著降低;IgG、C3、C4血漿濃度以及CD4+、CD8+T淋巴細胞比例在術後鑑測中,各組差異均無統計學意義(P＞0.05).結論 射頻保脾對機體短期內體液免疫功能無明顯損害,脾切除術後對機體短期體液免疫功能有損害;而無論是射頻保脾還是脾髒切除對機體遠期體液免疫功能及細胞免疫功能可能無明顯影響.
목적 관찰사빈보비급비절제술후궤체면역공능적개변.방법 사용수궤분조적방법장실험용장백저분위정상대조조(10두)、사빈보비조(10두)、비절제조(10두).분별우술후1、4、12、24주채정맥혈검측궤체면역공능지표:사용매련면역흡부시험(ELISA)법검측혈청IgM、IgG、C3、C4수평;사용류식세포의검측외주혈T림파세포CD4+、CD8+비례.결과 재체액면역방면,IgM수평재사빈보비술후무명현변화,이비절제조술후IgM수평현저강저;IgG、C3、C4혈장농도이급CD4+、CD8+T림파세포비례재술후감측중,각조차이균무통계학의의(P＞0.05).결론 사빈보비대궤체단기내체액면역공능무명현손해,비절제술후대궤체단기체액면역공능유손해;이무론시사빈보비환시비장절제대궤체원기체액면역공능급세포면역공능가능무명현영향.
Objective To evaluate the difference of immune function between normal control group,RF spleen preserving group (experimental group) and splenectomy group (experimental group),to explore the immune function of spleen preserving and after splenectomy changes,to provide some experimental basis for the clinical development of the spleen preserving operation.Methods Landrace pigs were divided into normal control group (10 cases),RF spleen preserving group (10 cases),spleen resection group (10 cases) by randomized.We detected immune function indexes respectively after 1 weeks,4 weeks,12 weeks,24 weeks in venous blood.We detected serum IgM,IgG,C3,C4 by enzyme linked immunosorbent assay (ELISA) method; T lymphocyte CD4 + and CD8 + ratio by blood flow cytometry.Results There was no obvious changes of IgM levels in RF spleen preserving group.IgM level decreased significantly after spleen resection.The IgG,C3,C4 and the percentage of CD4 + and CD8 + T lymphocytes showed no significant differences between the groups.Conclusion Compared with splenectomy,RF spleen preserving has no damage to short term humoral immunity.There was no effect on the long-term humoral immunity and cellular immune functionmight after both RF spleen preserving and splenectomy.
