中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
12期
2770-2771
,共2页
呼吸机相关肺损伤%急性肺损伤%丙泊酚%Beclin-1蛋白
呼吸機相關肺損傷%急性肺損傷%丙泊酚%Beclin-1蛋白
호흡궤상관폐손상%급성폐손상%병박분%Beclin-1단백
Ventilator-induced lung injury%Acute lung injury%Propofol%Beclin-1
目的 探讨丙泊酚预先给药对脂多糖(LPS)所致急性肺损伤的影响及其机制.方法 将45只雄性SD大鼠随机分为3组:对照组(C组)、LPS组(L组)和LPS+丙泊酚组(LP组),每组15只.C组尾静脉注射生理盐水;L组尾静脉注射LPS 7.5 mg/kg;LP组在尾静脉注射LPS7.5 mg/kg的基础上加用丙泊酚30 mg/kg.实验结束后放血处死大鼠,取肺组织进行病理学检查并测定肺湿/干重比(W/D);计算肺湿/干比重比值(W/D比值)、MPO活性及肺泡灌洗液中总蛋白含量,光镜下行WBC计数,同时Western blot检测肺组织中Beclin-1表达.结果 与C组比较,L组肺组织中W/D比值、WBC计数及总蛋白含量[5.21±0.27、(6.57 ±1.25)×106/L、(2.01±0.32)g/ml]均明显升高(P＜0.05),Beclin-1蛋白的表达水平(0.31 ±0.11)明显升高;与L组比较,W/D比值、WBC计数及总蛋白含量[4.41 ±0.21、(2.67±0.38)×106/L、(0.27 ±0.11) g/ml]明显降低(P＜0.05),Beclin-1蛋白的表达水平(0.47 ±0.15)亦明显升高.结论 丙泊酚具有肺保护作用,且该作用可能与其增加细胞内自噬相关基因Beclin-1表达有关.
目的 探討丙泊酚預先給藥對脂多糖(LPS)所緻急性肺損傷的影響及其機製.方法 將45隻雄性SD大鼠隨機分為3組:對照組(C組)、LPS組(L組)和LPS+丙泊酚組(LP組),每組15隻.C組尾靜脈註射生理鹽水;L組尾靜脈註射LPS 7.5 mg/kg;LP組在尾靜脈註射LPS7.5 mg/kg的基礎上加用丙泊酚30 mg/kg.實驗結束後放血處死大鼠,取肺組織進行病理學檢查併測定肺濕/榦重比(W/D);計算肺濕/榦比重比值(W/D比值)、MPO活性及肺泡灌洗液中總蛋白含量,光鏡下行WBC計數,同時Western blot檢測肺組織中Beclin-1錶達.結果 與C組比較,L組肺組織中W/D比值、WBC計數及總蛋白含量[5.21±0.27、(6.57 ±1.25)×106/L、(2.01±0.32)g/ml]均明顯升高(P＜0.05),Beclin-1蛋白的錶達水平(0.31 ±0.11)明顯升高;與L組比較,W/D比值、WBC計數及總蛋白含量[4.41 ±0.21、(2.67±0.38)×106/L、(0.27 ±0.11) g/ml]明顯降低(P＜0.05),Beclin-1蛋白的錶達水平(0.47 ±0.15)亦明顯升高.結論 丙泊酚具有肺保護作用,且該作用可能與其增加細胞內自噬相關基因Beclin-1錶達有關.
목적 탐토병박분예선급약대지다당(LPS)소치급성폐손상적영향급기궤제.방법 장45지웅성SD대서수궤분위3조:대조조(C조)、LPS조(L조)화LPS+병박분조(LP조),매조15지.C조미정맥주사생리염수;L조미정맥주사LPS 7.5 mg/kg;LP조재미정맥주사LPS7.5 mg/kg적기출상가용병박분30 mg/kg.실험결속후방혈처사대서,취폐조직진행병이학검사병측정폐습/간중비(W/D);계산폐습/간비중비치(W/D비치)、MPO활성급폐포관세액중총단백함량,광경하행WBC계수,동시Western blot검측폐조직중Beclin-1표체.결과 여C조비교,L조폐조직중W/D비치、WBC계수급총단백함량[5.21±0.27、(6.57 ±1.25)×106/L、(2.01±0.32)g/ml]균명현승고(P＜0.05),Beclin-1단백적표체수평(0.31 ±0.11)명현승고;여L조비교,W/D비치、WBC계수급총단백함량[4.41 ±0.21、(2.67±0.38)×106/L、(0.27 ±0.11) g/ml]명현강저(P＜0.05),Beclin-1단백적표체수평(0.47 ±0.15)역명현승고.결론 병박분구유폐보호작용,차해작용가능여기증가세포내자서상관기인Beclin-1표체유관.
Objective To investigate the protective effect of propofol pretreatment in a rat model of acute lung injury and the possible mechanisms.Methods Forty-five male SD rats weighing [(250-280) g] were randomly divided into 3 groups (n =15 each):control group (group C),lipopolysaccharide (LPS) group (group L) and LPS ± propofol group (group LP).Saline solution was injected into rats of group C through caudal vein.LPS (7.5 mg/kg) was injected into rats of group LP through caudal vein.LPS (7.5 mg/kg) and propofol (30 mg/kg) were injected into the rats of group LP through caudal vein at the same time.After the experiment,the lung wet-to-dry weight ratio (W/D),total protein (TP) contents,and counts of white blood cells (WBC) in broncho-alveolar lavage fluid (BALF) were determined.The expression of Becin1 was measured by Western blotting.Results As compared with group C,the W/D,WBC counts,TP contents and expression of Beclin-1 were significantly increased in group L [5.21 ± 0.27,(6.57 ± 1.25) × 106/L,(2.01 ± 0.32) g/ml and 0.31 ± 0.11 respectively).As compared with group L,the W/D,WBC counts,TP contents and expression of Beclin-1 were significantly increased in group L [4.41 ±0.21,(2.67 ±0.38) × 106/L,(0.27 ±0.11) g/ml and 0.47 ±0.15 respectively].Conclusion Propofol exerts a lung protective effect in the rats of ALI,which may be associated with the expression of autophagy related protein Beclin-1.
