中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
12期
2779-2781
,共3页
曹艳丽%张卫%张文霞%弓胜凯
曹豔麗%張衛%張文霞%弓勝凱
조염려%장위%장문하%궁성개
右美托咪啶%心肺复苏%缺血缺氧性脑损伤
右美託咪啶%心肺複囌%缺血缺氧性腦損傷
우미탁미정%심폐복소%결혈결양성뇌손상
Dexmedetomidine%Cardiopulmonary resuscitation%Hypoxic ischemic brain damage
目的 观察右美托咪啶对心肺复苏后大鼠血清硫化氢(H2S)、神经元特异性烯醇化酶(NSE)及S100β浓度的影响.方法 将60只健康的雄性大鼠分为3组:假手术组(A组)、常规复苏组(B组)、右美托咪啶组(C组)各20只,比较各组大鼠窒息前和心肺复苏后的PaO2、PaCO2水平,测定各组大鼠心肺复苏后的神经功能缺损评分(NDS)和血清H2S、NSE与S100β浓度.结果 经过心肺复苏后,B、C两组大鼠PaO2 [(45.48 ±5.98)和(48.26 ±6.93) mmHg(1 mmHg=0.133 kPa)]较窒息前[(92.15±2.87)和(91.89±2.76) mmHg]明显降低,PaCO2[(57.32±7.15)和(53.68 ±3.58) mmHg]较窒息前[(32.98±2.14)和(32.70±3.32) mmHg]明显升高(P＜0.05).B、C组大鼠的不同时点的NDS评分差异有统计学意义(P＜0.05);B、C组比较,第24、48和72小时的NDS评分(45.97±5.78、41.32±4.79、40.56±4.43和32.25±4.22、25.82±3.98、17.96±2.79)差异有统计学意义(P＜0.05).B、C组大鼠的不同时点的H2S、NSE与S100β浓度差异有统计学意义(P＜0.05);B、C组比较,第4、12、24、48和72小时的H2S和NSE浓度差异有统计学意义(P＜0.05);B、C组比较,第12、24、48和72小时的S100β浓度差异有统计学意义(P＜0.05).结论 右美托咪啶增加大鼠血清中H2S的浓度,降低NSE和S100β的浓度,对心肺复苏大鼠具有一定程度的脑保护作用.
目的 觀察右美託咪啶對心肺複囌後大鼠血清硫化氫(H2S)、神經元特異性烯醇化酶(NSE)及S100β濃度的影響.方法 將60隻健康的雄性大鼠分為3組:假手術組(A組)、常規複囌組(B組)、右美託咪啶組(C組)各20隻,比較各組大鼠窒息前和心肺複囌後的PaO2、PaCO2水平,測定各組大鼠心肺複囌後的神經功能缺損評分(NDS)和血清H2S、NSE與S100β濃度.結果 經過心肺複囌後,B、C兩組大鼠PaO2 [(45.48 ±5.98)和(48.26 ±6.93) mmHg(1 mmHg=0.133 kPa)]較窒息前[(92.15±2.87)和(91.89±2.76) mmHg]明顯降低,PaCO2[(57.32±7.15)和(53.68 ±3.58) mmHg]較窒息前[(32.98±2.14)和(32.70±3.32) mmHg]明顯升高(P＜0.05).B、C組大鼠的不同時點的NDS評分差異有統計學意義(P＜0.05);B、C組比較,第24、48和72小時的NDS評分(45.97±5.78、41.32±4.79、40.56±4.43和32.25±4.22、25.82±3.98、17.96±2.79)差異有統計學意義(P＜0.05).B、C組大鼠的不同時點的H2S、NSE與S100β濃度差異有統計學意義(P＜0.05);B、C組比較,第4、12、24、48和72小時的H2S和NSE濃度差異有統計學意義(P＜0.05);B、C組比較,第12、24、48和72小時的S100β濃度差異有統計學意義(P＜0.05).結論 右美託咪啶增加大鼠血清中H2S的濃度,降低NSE和S100β的濃度,對心肺複囌大鼠具有一定程度的腦保護作用.
목적 관찰우미탁미정대심폐복소후대서혈청류화경(H2S)、신경원특이성희순화매(NSE)급S100β농도적영향.방법 장60지건강적웅성대서분위3조:가수술조(A조)、상규복소조(B조)、우미탁미정조(C조)각20지,비교각조대서질식전화심폐복소후적PaO2、PaCO2수평,측정각조대서심폐복소후적신경공능결손평분(NDS)화혈청H2S、NSE여S100β농도.결과 경과심폐복소후,B、C량조대서PaO2 [(45.48 ±5.98)화(48.26 ±6.93) mmHg(1 mmHg=0.133 kPa)]교질식전[(92.15±2.87)화(91.89±2.76) mmHg]명현강저,PaCO2[(57.32±7.15)화(53.68 ±3.58) mmHg]교질식전[(32.98±2.14)화(32.70±3.32) mmHg]명현승고(P＜0.05).B、C조대서적불동시점적NDS평분차이유통계학의의(P＜0.05);B、C조비교,제24、48화72소시적NDS평분(45.97±5.78、41.32±4.79、40.56±4.43화32.25±4.22、25.82±3.98、17.96±2.79)차이유통계학의의(P＜0.05).B、C조대서적불동시점적H2S、NSE여S100β농도차이유통계학의의(P＜0.05);B、C조비교,제4、12、24、48화72소시적H2S화NSE농도차이유통계학의의(P＜0.05);B、C조비교,제12、24、48화72소시적S100β농도차이유통계학의의(P＜0.05).결론 우미탁미정증가대서혈청중H2S적농도,강저NSE화S100β적농도,대심폐복소대서구유일정정도적뇌보호작용.
Objective To study the effect of dexmedetomidine on concentrations of hydrogen sulfide (H2S),neuron-specific enolase (NSE) and S100β after cardio-pulmonary resuscitation (CPR) in rats.Methods Sixty SD rats were devided into three groups:sham group (group A),conventional resuscitation group (group B) and dexmedetomidine group (group C) (n =20 each).Blood gas analysis before was compared before asphyxia and after resuscitation among 3 groups.The neurologic deficit scoring (NDS) and the concentrations of H2S,NSE and S100β were compared also.Results After resuscitation,the PaO2 in groups B and C [(45.48 ±5.98) and (48.26 ±6.93) mmHg (1 mmHg =0.133 kPa)] was decreased as compared with before asphyxia [(92.15 ±2.87) and (91.89 ±2.76) mmHg],and PaCO2 [(57.32 ±7.15) and (53.68 ±3.58) mmHg] increased as compared with before asphyxia [(32.98 ± 2.14) and (32.70 ±3.32) mmHg,P ＜0.05].There was significant difference in NDS concentration between groups B and C at different time points (P ＜ 0.05).At 24,48 and 72 h after CPR,there was significant difference in NDS concentration between groups B and C (45.97 ± 5.78,41.32 ±4.79,40.56 ±4.43 and 32.25 ± 4.22,25.82 ± 3.98,17.96 ± 2.79,P ＜ 0.05).There was significant difference in the concentrations of H2S,NSE and S100β between groups B and C at different time points (P ＜ 0.05).At 4,12,24,48 and 72 h after CPR,there was statistically significant difference in H2S and NSE concentrations between groups B and C (P ＜0.05) ; at 12,24,48 and 72 h after CPR,there was significant difference in S100β concentration between groups B and C (P ＜ 0.05).Conclusion Dexmedetomidine can increase the concentrations of H2S as well as reduce the concentrations of NSE and S100β in serum,and it has protective effects on brain of rats to a certain extent.
