中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
12期
2792-2795
,共4页
陆义芹%唐传劲%张小超%张悦%何波%沈志强%陈鹏
陸義芹%唐傳勁%張小超%張悅%何波%瀋誌彊%陳鵬
륙의근%당전경%장소초%장열%하파%침지강%진붕
老鹳草素%成骨细胞%骨保护素
老鸛草素%成骨細胞%骨保護素
로관초소%성골세포%골보호소
Geraniin%Osteoblast%Osteoprotegerin
目的 观察老鹳草素对成骨细胞增殖和骨保护素(OPG)表达的影响.方法 培养小鼠成骨细胞MC3T3-E1,噻唑蓝(MTT)法观测老鹳草素对成骨细胞增殖能力的影响.免疫细胞化学染色法和原位杂交技术检测老鹳草素对成骨细胞OPG蛋白和mRNA表达的影响.结果 MTT法测得对照组成骨细胞吸光度值为0.777±0.088、10-12 ～ 10-8 mol/L老鹳草素组分别为0.958±0.054、0.943±0.029、0.918±0.070、0.899±0.048和0.793±0.074;与对照组比较,除10-8 mol/L组外,差异有统计学意义(P＜0.01).对照组和10-11～10-8 mol/L老鹳草素组OPG蛋白平均吸光度值分别为0.231±0.022、0.249±0.015、0.255±0.021、0.302±0.025和0.328±0.021;OPG mRNA平均吸光度值分别为0.297 ±0.041、0.307±0.039、0.318 ±0.035、0.394±0.036和0.429±0.050,提示老鹳草素呈浓度依赖性上调成骨细胞OPG表达.结论 老鹳草素具有促进成骨细胞增殖的作用,OPG表达上调可能是其中的机制之一.
目的 觀察老鸛草素對成骨細胞增殖和骨保護素(OPG)錶達的影響.方法 培養小鼠成骨細胞MC3T3-E1,噻唑藍(MTT)法觀測老鸛草素對成骨細胞增殖能力的影響.免疫細胞化學染色法和原位雜交技術檢測老鸛草素對成骨細胞OPG蛋白和mRNA錶達的影響.結果 MTT法測得對照組成骨細胞吸光度值為0.777±0.088、10-12 ～ 10-8 mol/L老鸛草素組分彆為0.958±0.054、0.943±0.029、0.918±0.070、0.899±0.048和0.793±0.074;與對照組比較,除10-8 mol/L組外,差異有統計學意義(P＜0.01).對照組和10-11～10-8 mol/L老鸛草素組OPG蛋白平均吸光度值分彆為0.231±0.022、0.249±0.015、0.255±0.021、0.302±0.025和0.328±0.021;OPG mRNA平均吸光度值分彆為0.297 ±0.041、0.307±0.039、0.318 ±0.035、0.394±0.036和0.429±0.050,提示老鸛草素呈濃度依賴性上調成骨細胞OPG錶達.結論 老鸛草素具有促進成骨細胞增殖的作用,OPG錶達上調可能是其中的機製之一.
목적 관찰로관초소대성골세포증식화골보호소(OPG)표체적영향.방법 배양소서성골세포MC3T3-E1,새서람(MTT)법관측로관초소대성골세포증식능력적영향.면역세포화학염색법화원위잡교기술검측로관초소대성골세포OPG단백화mRNA표체적영향.결과 MTT법측득대조조성골세포흡광도치위0.777±0.088、10-12 ～ 10-8 mol/L로관초소조분별위0.958±0.054、0.943±0.029、0.918±0.070、0.899±0.048화0.793±0.074;여대조조비교,제10-8 mol/L조외,차이유통계학의의(P＜0.01).대조조화10-11～10-8 mol/L로관초소조OPG단백평균흡광도치분별위0.231±0.022、0.249±0.015、0.255±0.021、0.302±0.025화0.328±0.021;OPG mRNA평균흡광도치분별위0.297 ±0.041、0.307±0.039、0.318 ±0.035、0.394±0.036화0.429±0.050,제시로관초소정농도의뢰성상조성골세포OPG표체.결론 로관초소구유촉진성골세포증식적작용,OPG표체상조가능시기중적궤제지일.
Objective To observe the effects of geraniin on osteoblastic proliferation and osteoprotegerin (OPG) expression.Methods The osteoblasts (MC3T3-E1) were cultured with different concentrations of geraniin.Cell proliferation was assessed by methyl thiazolyl tetrazolium (MTT) colorimetric assay.The protein and mRNA expression levels of OPG were measured by immunocytochemistry and in situ hybridization (ISH) respectively.Results The absorbance values of the control group and geraniin (10-12-10-8mol/L) groups were 0.777 ±0.088,0.958 ±0.054,0.943 ±0.029,0.918 ±0.070,0.899 ± 0.04 and 0.793 ± 0.074 respectively with the differences being statistically significant between control group and geraniin groups except 10-8 mol/L geraniin group.The average absorbance values of OPG protein in the control group and geraniin (10-12-10-8 mol/L) groups were 0.231 ± 0.022,0.249 ± 0.015,0.255 ±0.021,0.302 ± 0.025 and 0.328 ± 0.021 respectively; and those of OPG mRNA were 0.297 ±0.041,0.307 ±0.039,0.318 ±0.035,0.394 ±0.036 and 0.429 ±0.050 respectively,showing geraniin promoted the protein and mRNA expression of OPG in a concentration-dependent manner.Conclusion It suggests that geraniin promotes osteoblast proliferation and upregulates the protein and mRNA expression of OPG.
