中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
12期
2825-2827
,共3页
尤小兰%王元杰%王伟林%余翔%喻春钊
尤小蘭%王元傑%王偉林%餘翔%喻春釗
우소란%왕원걸%왕위림%여상%유춘쇠
乳腺癌%微转移%上皮小黏蛋白%人乳珠蛋白%生存率
乳腺癌%微轉移%上皮小黏蛋白%人乳珠蛋白%生存率
유선암%미전이%상피소점단백%인유주단백%생존솔
Breast cancer%Micrometastatic%Small breast epithelial mucin%Humam mamaglobin%Survival rates
目的 探讨外周血中乳腺上皮小黏蛋白(SBEM) mRNA、人乳珠蛋白(hMAM) mRNA的表达水平与乳腺癌预后的关系.方法 利用Nested-逆转录-聚合酶链反应(RT-PCR)技术检测92例乳腺癌术后患者,40例非乳腺癌患者外周血SBEM、hMAM mRNA,并对92例乳腺癌患者进行临床随访分析.结果 SBEM、hMAM mRNA仅在乳腺恶性肿瘤外周血中表达,Kaplan-Meier生存分析结果显示:乳腺癌患者外周血中SBEM mRNA阳性表达组生存率47.7％小于阴性组85.1％(P＜0.01),hMAM mRNA阳性表达组生存率46.3％小于阴性组84.0％ (P＜ 0.01),差异有统计学意义.SBEM、hMAM mRNA阳性表达有相关(P＜0.01).结论 外周血中SBEM、hMAM mRNA检测有助于判断乳腺癌预后,两种肿瘤标志物的联合检测提高了检测灵敏度.
目的 探討外週血中乳腺上皮小黏蛋白(SBEM) mRNA、人乳珠蛋白(hMAM) mRNA的錶達水平與乳腺癌預後的關繫.方法 利用Nested-逆轉錄-聚閤酶鏈反應(RT-PCR)技術檢測92例乳腺癌術後患者,40例非乳腺癌患者外週血SBEM、hMAM mRNA,併對92例乳腺癌患者進行臨床隨訪分析.結果 SBEM、hMAM mRNA僅在乳腺噁性腫瘤外週血中錶達,Kaplan-Meier生存分析結果顯示:乳腺癌患者外週血中SBEM mRNA暘性錶達組生存率47.7％小于陰性組85.1％(P＜0.01),hMAM mRNA暘性錶達組生存率46.3％小于陰性組84.0％ (P＜ 0.01),差異有統計學意義.SBEM、hMAM mRNA暘性錶達有相關(P＜0.01).結論 外週血中SBEM、hMAM mRNA檢測有助于判斷乳腺癌預後,兩種腫瘤標誌物的聯閤檢測提高瞭檢測靈敏度.
목적 탐토외주혈중유선상피소점단백(SBEM) mRNA、인유주단백(hMAM) mRNA적표체수평여유선암예후적관계.방법 이용Nested-역전록-취합매련반응(RT-PCR)기술검측92례유선암술후환자,40례비유선암환자외주혈SBEM、hMAM mRNA,병대92례유선암환자진행림상수방분석.결과 SBEM、hMAM mRNA부재유선악성종류외주혈중표체,Kaplan-Meier생존분석결과현시:유선암환자외주혈중SBEM mRNA양성표체조생존솔47.7％소우음성조85.1％(P＜0.01),hMAM mRNA양성표체조생존솔46.3％소우음성조84.0％ (P＜ 0.01),차이유통계학의의.SBEM、hMAM mRNA양성표체유상관(P＜0.01).결론 외주혈중SBEM、hMAM mRNA검측유조우판단유선암예후,량충종류표지물적연합검측제고료검측령민도.
Objective To explore the correlation between the prognosis of breast cancer and small breast epithelial mucin (SBEM) mRNA,humam mamaglobin (hMAM) mRNA in the peripheral blood of the patients with breast cancer.Methods Using the N ested-reverse transcriptase-polymerase chain reaction (RT-PCR) technique to detect the SBEM mRNA,hMAM mRNA in peripheral blood.92 cases were postoperative patients of breast cancer,40 cases were not patients with breast cancer.And clinical followup analysis of 92 cases of patients with breast cancer.Results SBEM mRNA,hMAM mRNA only expressed in the peripheral blood of the patients with breast cancer.The survival rate of the breast cancer patients with positive expression of SBEM mRNA was 47.7％ less than the negative group 85.1％ (P ＜0.01) in peripheral blood,The survival rate of the breast cancer patients with positive expression of hMAM mRNA was 46.3％ less than the negative group 85.1％ (P ＜0.01),The correlation statistical was significance between SBEM mRNA and hMAM mRNA positive expression in peripheral blood (P ＜0.01).Conclusion Detection SBEM mRNA,hMAM mRNA in peripheral blood is a clinical indicators for breast cancer prognosis,two kinds of joint detection of tumor markers can improve the detection sensitivity.Combined detection of two kinds of tumor markers can improve the detection sensitivity.
