CAJ | 학술논문

目的探讨乳腺癌患者血清中Survivin抗体的阳性率及其与乳腺癌患者肿瘤组织中P53表达的相关性;两者与临床病例诊断及预后因素分析的相关性.方法对92例乳腺癌患者的血清和肿瘤组织标本,分别应用酶联免疫吸附试验(ELISA)检测Survivin抗体的含量,免疫组织化学法检测p53的表达,并将这两种细胞因子与淋巴结转移及临床病理特征进行相关分析.结果 Survivin抗体阳性率为15.00％ (14/92),p53阳性率为34.78％ (32/92).两者与临床病理特征的相关分析结果表明,Survivin抗体和p53高表达与乳腺癌患者淋巴结转移呈负相关(P＜0.05).结论联合检测Survivin和p53蛋白表达有助于预测淋巴结转移及判断预后.
목적 탐토유선암환자혈청중Survivin항체적양성솔급기여유선암환자종류조직중P53표체적상관성;량자여림상병례진단급예후인소분석적상관성.방법 대92례유선암환자적혈청화종류조직표본,분별응용매련면역흡부시험(ELISA)검측Survivin항체적함량,면역조직화학법검측p53적표체,병장저량충세포인자여림파결전이급림상병리특정진행상관분석.결과 Survivin항체양성솔위15.00％ (14/92),p53양성솔위34.78％ (32/92).량자여림상병리특정적상관분석결과표명,Survivin항체화p53고표체여유선암환자림파결전이정부상관(P＜0.05).결론 연합검측Survivin화p53단백표체유조우예측림파결전이급판단예후.
Objective To investigate the positive rate of Survivin antibody expression in serum from breast cancer patients and its relationship with p53 expression in tumor tissues,and the correlation of Survivin and p53 with clinical diagnosis and prognostic factors.Methods The serum and tumor samples were obtained from 92 cases of breast cancer.Enzyme-linked immunosorbent assay (ELISA) was used to detect the concentration of Survivin antibody in serum,and immunohistochemistry to detect the p53 expression in tumor tissues.The correlation of Survivin and p53 with both tumor lymph node metastasis and clinicopathologic reports was analyzed.Results Positive rate of Survivin antibody and p53 protein was 15.00％ and 34.78％ respectively.The high expression of Survivin antibody and p53 was negatively correlated with lymph node metastasis in patients with breast cancer.Conclusion Combined detection of Survivin and p53 expression can be used as an indicator to predict lymph node metastasis and prognosis.