CAJ | 학술논문

目的探讨人类白细胞抗原(HLA)-DQA1基因拷贝数多态性与胃癌易感性的相关性,以及其与幽门螺杆菌(Hp)感染在胃癌发生中的交互作用.方法利用微阵列-比较基因组杂交定位和AccuCopy多重基因拷贝数检测技术,对53例经病理检查确诊的胃癌患者和50例体检的正常人群的HLA-DQA1基因拷贝数多态性进行检测.利用Logistic回归模型分析HLA-DQA1基因拷贝数多态性与胃癌易感性的关系,以及与Hp感染的交互作用对胃癌易感性的影响.结果 HLA-DQA1基因拷贝数多态性分布和Hp感染阳性率在两组对象中不同,差异有统计学意义(P ＜0.05);HLA-DQA1基因拷贝数＞2型人群胃癌易感性显著增加[比值比(OR)=1.79,95％可信区间(CI):1.13 ～ 3.22,P＜0.05];HLA-DQA1基因拷贝数＞2且Hp感染阳性人群胃癌易感性显著增加(OR=3.31,95％CI:1.69～7.88,P ＜0.05).结论 HLA-DQA1基因拷贝数多态性与胃癌易感性密切相关,而与Hp感染无明显相关,但两者在胃癌发生进展过程中共同发挥交互作用.
목적 탐토인류백세포항원(HLA)-DQA1기인고패수다태성여위암역감성적상관성,이급기여유문라간균(Hp)감염재위암발생중적교호작용.방법 이용미진렬-비교기인조잡교정위화AccuCopy다중기인고패수검측기술,대53례경병리검사학진적위암환자화50례체검적정상인군적HLA-DQA1기인고패수다태성진행검측.이용Logistic회귀모형분석HLA-DQA1기인고패수다태성여위암역감성적관계,이급여Hp감염적교호작용대위암역감성적영향.결과 HLA-DQA1기인고패수다태성분포화Hp감염양성솔재량조대상중불동,차이유통계학의의(P ＜0.05);HLA-DQA1기인고패수＞2형인군위암역감성현저증가[비치비(OR)=1.79,95％가신구간(CI):1.13 ～ 3.22,P＜0.05];HLA-DQA1기인고패수＞2차Hp감염양성인군위암역감성현저증가(OR=3.31,95％CI:1.69～7.88,P ＜0.05).결론 HLA-DQA1기인고패수다태성여위암역감성밀절상관,이여Hp감염무명현상관,단량자재위암발생진전과정중공동발휘교호작용.
Objective To investigate the correlation of human leukocyte antigen (HLA)-DQA1 gene copy number polymorphisms and susceptibility to gastric cancer,and to explore their interaction with Helicobacterpylori (Hp) infection in gastric carcinogenesis.Methods HLA-DQA1 gene copy number in 53 cases of pathologically diagnosed gastric cancer and 50 cases of normal healthy population was detected by using microarray-comparative hybridization and AccuCopy multiple gene copy number detection technology.Logistic regression analysis was used to evaluate the impact of this polymorphism on the risk of susceptibility to gastric cancer and the gene-Hp infection interaction.Results There was statistically significant difference in the HLA-DQA1 gene copy number polymorphisms and positive rate of Hp infection between the two groups (P ＜ 0.05).The susceptibility of population to gastric cancer with HLA-DQA1 gene copy number ＞2-type was significantly increased [odds ratio (OR) =1.79,95％ confidence interval (CI):1.13-3.22,P ＜0.05).The susceptibility of population to gastric cancer with HLA-DQA1 gene copy number ＞ 2-type and Hp positive infection was significantly increased (OR =3.31,95％ CI:1.69-7.88,P ＜ 0.05).Conclusion HLA-DQA1 gene copy number polymorphisms were closely related with susceptibility to gastric cancer,while were not with Hp infection,but there was a multiplicative interaction between these polymorphisms and Hp infection in the process of gastric cancer.